PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_1611 [new locus tag: NWMN_RS09035 ]
• pan locus tag^?: SAUPAN004370000
• symbol: ezrA
• pan gene symbol^?: ezrA
• synonym:
• product: septation ring formation regulator EzrA

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_1611 [new locus tag: NWMN_RS09035 ]
• symbol: ezrA
• product: septation ring formation regulator EzrA
• replicon: chromosome
• strand: -
• coordinates: 1788838..1790532
• length: 1695
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5330946 NCBI
• RefSeq: YP_001332645 NCBI
• BioCyc:
• MicrobesOnline: 3707163 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  ATGGTGTTATATATCATTTTGGCAATAATTGTGATTATATTGATTGCTGTAGGTGTATTA
  TTCTATTTACGTTCAAATAAACGACAAATTATTGAAAAAGCAATCGAACGTAAAAATGAA
  ATTGAGACGTTACCTTTTGATCAAAACCTTGCACAATTATCTAAGTTGAATTTAAAAGGT
  GAAACAAAAACGAAATACGATGCAATGAAAAAGGACAACGTAGAAAGTACAAATAAGTAT
  CTAGCTCCTGTGGAAGAAAAAATCCATAATGCTGAGGCTTTATTAGATAAATTTAGTTTC
  AACGCATCTCAAAGTGAAATTGATGATGCAAATGAGTTGATGGATAGTTACGAACAAAGC
  TATCAGCAACAATTAGAAGATGTAAATGAAATTATTGCGTTATACAAAGATAATGATGAA
  TTATATGACAAATGTAAGGTTGATTATCGTGAAATGAAACGTGATGTTTTAGCAAATCGT
  CATCAATTTGGTGAGGCAGCAAGTCTACTTGAAACTGAAATTGAAAAATTTGAGCCAAGG
  TTAGAGCAATATGAAGTACTAAAAGCTGATGGTAATTATGTACAAGCGCACAACCATATA
  GCTGCCTTGAATGAACAAATGAAACAGCTAAGATCTTATATGGAAGAAATACCAGAATTA
  ATTAGAGAAACTCAAAAAGAATTACCTGGTCAATTCCAAGATTTAAAATATGGTTGCCGT
  GATCTTAAAGTTGAAGGGTATGATCTGGATCACGTGAAAGTAGACAGTACATTACAAAGC
  TTAAAAACAGAGCTTAGTTTCGTTGAACCATTAATTAGCCGCTTAGAATTAGAAGAAGCT
  AATGATAAACTAGCTAATATCAATGATAAGTTAGATGACATGTATGATTTAATTGAACAT
  GAAGTTAAAGCTAAAAATGATGTCGAAGAAACAAAAGATATCATTACGGATAACTTATTC
  AAAGCTAAAGACATGAATTATACATTGCAAACAGAAATTGAATATGTACGTGAAAACTAC
  TATATAAATGAATCTGATGCTCAGAGTGTTCGTCAATTTGAAAATGAAATTCAAAGTTTA
  ATTTCTGTATATGATGATATTTTAAAAGAAATGTCTAAATCTGCTGTGCGATATAGCGAG
  GTTCAGGATAATTTACAATATTTAGAAGATCATGTCACAGTTATTAATGACAAACAAGAA
  AAGCTACAAAATCATCTGATTCAATTGCGTGAAGATGAAGCAGAAGCAGAAGACAATCTG
  CTACGAGTACAATCGAAGAAAGAAGAAGTGTATCGTCGATTACTTGCTTCTAACTTAACA
  AGCGTTCCTGAAAGGTTTATCATCATGAAAAATGAAATTGATCATGAAGTTCGTGATGTT
  AACGAACAATTTAGTGAACGTCCAATACACGTTAAACAGTTAAAAGATAAAGTGTCTAAA
  ATTGTGATTCAAATGAATACATTTGAAGATGAAGCAAATGATGTTCTTGTTAATGCTGTT
  TATGCAGAGAAATTAATTCAATATGGAAATAGATATCGTAAGGACTATAGCAATGTTGAT
  AAGAGCTTAAATGAAGCTGAACGATTATTTAAAAATAATCGCTATAAGCGTGCGATTGAA
  ATTGCAGAGCAAGCTCTTGAAAGTGTTGAGCCAGGTGTTACTAAACATATTGAAGAAGAA
  GTTATTAAGCAATAG
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⊟Protein

⊟General

• locus tag: NWMN_1611 [new locus tag: NWMN_RS09035 ]
• symbol: EzrA
• description: septation ring formation regulator EzrA
• length: 564
• theoretical pI: 4.50272
• theoretical MW: 66199
• GRAVY: -0.686348

⊟Function

• TIGRFAM:
  Cellular processes Cellular processes Cell division chromosome segregation protein SMC (TIGR02168; HMM-score: 22.7)
  Genetic information processing DNA metabolism Chromosome-associated proteins chromosome segregation protein SMC (TIGR02168; HMM-score: 22.7)
  Cellular processes Cellular processes Cell division chromosome segregation protein SMC (TIGR02169; HMM-score: 22.7)
  Genetic information processing DNA metabolism Chromosome-associated proteins chromosome segregation protein SMC (TIGR02169; HMM-score: 22.7)
  and 2 more
  Genetic information processing Protein fate Protein folding and stabilization cytochrome c-type biogenesis protein CcmI (TIGR03142; HMM-score: 5.9)
  Metabolism Energy metabolism Electron transport cytochrome c-type biogenesis protein CcmI (TIGR03142; HMM-score: 5.9)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  no clan defined EzrA; Septation ring formation regulator, EzrA (PF06160; HMM-score: 529.1)
  and 12 more
  PI3K_P85_iSH2; Phosphatidylinositol 3-kinase regulatory subunit P85 inter-SH2 domain (PF16454; HMM-score: 22.7)
  GPW_gp25; Gene 25-like lysozyme (PF04965; HMM-score: 18.1)
  Ac110_PIF; Per os infectivity factor AC110 (PF07280; HMM-score: 15.6)
  TPR (CL0020) TPR_2; Tetratricopeptide repeat (PF07719; HMM-score: 12.5)
  no clan defined ASFV_J13L; African swine fever virus J13L protein (PF05568; HMM-score: 12.4)
  DUF2106; Predicted membrane protein (DUF2106) (PF09879; HMM-score: 12)
  TPR (CL0020) ANAPC3; Anaphase-promoting complex, cyclosome, subunit 3 (PF12895; HMM-score: 12)
  TPR_16; Tetratricopeptide repeat (PF13432; HMM-score: 11.1)
  no clan defined Med9; RNA polymerase II transcription mediator complex subunit 9 (PF07544; HMM-score: 10.8)
  IncA; IncA protein (PF04156; HMM-score: 9.3)
  SIT; SHP2-interacting transmembrane adaptor protein, SIT (PF15330; HMM-score: 9.2)
  HTH (CL0123) MerR-DNA-bind; MerR, DNA binding (PF09278; HMM-score: 7.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 10
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helix: 1
• LocateP: N-terminally anchored (No CS)
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 4
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.026729
  • TAT(Tat/SPI): 0.000705
  • LIPO(Sec/SPII): 0.007408
• predicted transmembrane helices (TMHMM): 1

⊟Accession numbers

• GI: 151221823 NCBI
• RefSeq: YP_001332645 NCBI
• UniProt: A6QHQ1 UniProt

⊟Protein sequence

• MVLYIILAIIVIILIAVGVLFYLRSNKRQIIEKAIERKNEIETLPFDQNLAQLSKLNLKGETKTKYDAMKKDNVESTNKYLAPVEEKIHNAEALLDKFSFNASQSEIDDANELMDSYEQSYQQQLEDVNEIIALYKDNDELYDKCKVDYREMKRDVLANRHQFGEAASLLETEIEKFEPRLEQYEVLKADGNYVQAHNHIAALNEQMKQLRSYMEEIPELIRETQKELPGQFQDLKYGCRDLKVEGYDLDHVKVDSTLQSLKTELSFVEPLISRLELEEANDKLANINDKLDDMYDLIEHEVKAKNDVEETKDIITDNLFKAKDMNYTLQTEIEYVRENYYINESDAQSVRQFENEIQSLISVYDDILKEMSKSAVRYSEVQDNLQYLEDHVTVINDKQEKLQNHLIQLREDEAEAEDNLLRVQSKKEEVYRRLLASNLTSVPERFIIMKNEIDHEVRDVNEQFSERPIHVKQLKDKVSKIVIQMNTFEDEANDVLVNAVYAEKLIQYGNRYRKDYSNVDKSLNEAERLFKNNRYKRAIEIAEQALESVEPGVTKHIEEEVIKQ

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications