COLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_1769 [new locus tag: NWMN_RS10115 ]
• pan locus tag^?:
• symbol: NWMN_1769
• pan gene symbol^?: —
• synonym:
• product: phage amidase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_1769 [new locus tag: NWMN_RS10115 ]
• symbol: NWMN_1769
• product: phage amidase
• replicon: chromosome
• strand: -
• coordinates: 1980874..1982328
• length: 1455
• essential: unknown

⊟Accession numbers

• Gene ID: 5331056 NCBI
• RefSeq: YP_001332803 NCBI
• BioCyc:
• MicrobesOnline: 3707357 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
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  301
  361
  421
  481
  541
  601
  661
  721
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  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  1381
  1441
  
  ATGTTAATGACAAAAAATCAAGCAGAAAAATGGTTTGACAATTCATTAGGGAAACAATTC
  AACCCAGATGGTTGGTATGGATTTCAGTGTTATGATTACGCCAATATGTTCTTTATGTTA
  GCGACAGGCGAAAGGCTGCAAGGTTTATATGCTTATAATATCCCGTTTGATAATAAAGCA
  AAGATTGAAAAATATGGTCAAATAATTAAAAACTATGACAGCTTTTTACCGCAAAAGTTG
  GATATTGTCGTTTTCCCGTCAAAGTATGGTGGCGGAGCTGGACACGTTGAAATTGTTGAG
  AGCGCAAATTTAAATACTTTCACATCATTTGGTCAAAACTGGAACGGTAAAGGTTGGACT
  AATGGCGTTGCGCAACCTGGTTGGGGTCCTGAAACTGTGACAAGACATGTTCATTATTAT
  GACAATCCAATGTATTTTATTAGGTTAAACTTCCCTAACAACTTAAGCGTTGGCAATAAA
  GCTAAAGGTATTATTAAGCAAGCGACTACAAAAAAAGAGGCAGTAATTAAACCTAAAAAA
  ATTATGCTTGTAGCCGGTCATGGTTATAACGATCCTGGAGCAGTAGGAAACGGAACAAAC
  GAACGCGATTTTATACGTAAATATATAACGCCTAATATCGCTAAGTATTTAAGACATGCA
  GGACATGAAGTTGCATTATACGGTGGCTCAAGTCAATCACAAGATATGTATCAAGATACT
  GCATACGGTGTTAATGTAGGCAATAAAAAAGATTATGGCTTATATTGGGTTAAATCACAG
  GGGTATGACATTGTTCTAGAAATACATTTAGACGCAGCAGGAGAAAGCGCAAGTGGTGGG
  CATGTTATTATCTCAAGTCAATTCAATGCAGATACTATTGATAAAAGTATACAAGATGTT
  ATTAAAAATAACTTAGGACAAATAAGAGGTGTGACACCTCGTAATGATTTACTAAATGTT
  AATGTATCAGCAGAAATAAATATAAATTATCGTTTATCTGAATTAGGTTTTATTACTAAT
  AAAAATGATATGGATTGGATTAAGAAAAACTATGACTTGTATTCTAAATTAATAGCCGGT
  GCGATTCATGGTAAGCCTATAGGTGGTTTGGTAGCTGGTAATGTTAAAACATCAGCTAAA
  AACAAAAAAAATCCACCAGTGCCAGCAGGTTATACACTCGATAAGAATAATGTCCCTTAT
  AAAAAAGAACAAGGCAATTACACAGTAGCTAATGTTAAAGGTAATAATGTAAGAGACGGT
  TATTCAACTAATTCAAGAATTACAGGGGTATTACCCAACAACACAACAATTACGTATGAC
  GGTGCATATTGTATTAATGGTTATAGATGGATTACTTATATTGCTAATAGTGGACAACGT
  CGTTATATAGCGACAGGAGAGGTAGACAAGGCAGGTAATAGAATAAGTAGTTTTGGTAAG
  TTTAGCACGATTTAG
  60
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  1140
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  1260
  1320
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  1440
  1455
  

⊟Protein

⊟General

• locus tag: NWMN_1769 [new locus tag: NWMN_RS10115 ]
• symbol: NWMN_1769
• description: phage amidase
• length: 484
• theoretical pI: 9.70541
• theoretical MW: 53800.2
• GRAVY: -0.509711

⊟Function

• TIGRFAM:
  Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan N-acetylmuramoyl-L-alanine amidase CwlD (TIGR02883; EC 3.5.1.28; HMM-score: 43.8)
  Cellular processes Cellular processes Sporulation and germination N-acetylmuramoyl-L-alanine amidase CwlD (TIGR02883; EC 3.5.1.28; HMM-score: 43.8)
• TheSEED:
• PFAM:
  Peptidase_MH (CL0035) Amidase_3; N-acetylmuramoyl-L-alanine amidase (PF01520; HMM-score: 136.8)
  and 3 more
  Peptidase_CA (CL0125) CHAP; CHAP domain (PF05257; HMM-score: 37.5)
  SH3 (CL0010) SH3_5; Bacterial SH3 domain (PF08460; HMM-score: 35.6)
  SH3_3; Bacterial SH3 domain (PF08239; HMM-score: 17.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.022393
  • TAT(Tat/SPI): 0.000344
  • LIPO(Sec/SPII): 0.001173
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151221981 NCBI
• RefSeq: YP_001332803 NCBI
• UniProt: A0A0H3KAP2 UniProt

⊟Protein sequence

• MLMTKNQAEKWFDNSLGKQFNPDGWYGFQCYDYANMFFMLATGERLQGLYAYNIPFDNKAKIEKYGQIIKNYDSFLPQKLDIVVFPSKYGGGAGHVEIVESANLNTFTSFGQNWNGKGWTNGVAQPGWGPETVTRHVHYYDNPMYFIRLNFPNNLSVGNKAKGIIKQATTKKEAVIKPKKIMLVAGHGYNDPGAVGNGTNERDFIRKYITPNIAKYLRHAGHEVALYGGSSQSQDMYQDTAYGVNVGNKKDYGLYWVKSQGYDIVLEIHLDAAGESASGGHVIISSQFNADTIDKSIQDVIKNNLGQIRGVTPRNDLLNVNVSAEININYRLSELGFITNKNDMDWIKKNYDLYSKLIAGAIHGKPIGGLVAGNVKTSAKNKKNPPVPAGYTLDKNNVPYKKEQGNYTVANVKGNNVRDGYSTNSRITGVLPNNTTITYDGAYCINGYRWITYIANSGQRRYIATGEVDKAGNRISSFGKFSTI

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: NWMN_1769 < NWMN_1770

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications