PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_1812 [new locus tag: NWMN_RS10410 ]
• pan locus tag^?: SAUPAN001766000
• symbol: NWMN_1812
• pan gene symbol^?: —
• synonym:
• product: phage repressor

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_1812 [new locus tag: NWMN_RS10410 ]
• symbol: NWMN_1812
• product: phage repressor
• replicon: chromosome
• strand: +
• coordinates: 2020253..2020891
• length: 639
• essential: unknown

⊟Accession numbers

• Gene ID: 5331099 NCBI
• RefSeq: YP_001332846 NCBI
• BioCyc:
• MicrobesOnline: 3707400 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGGCTTTCAAAAATTCCATAAAAGAAATCAGATTGAACAATAGATTGTCTAAAGTTGAG
  ATGGCTAAAAAATTAGATGTTTCCGAAGGTACTATAAGAATGTGGGAAAGTGGAAGAACT
  GAACCTAGAATGGGTATGGTCGAAAAAATTTCAAGTTTGTTCAATGTTTCTAAAGGTTAT
  CTCTTAGGAGAAATTGAAGAAATTGTTTTACCAGAATTTGATAGCGAAATCGAGGTTCCA
  TATTTCGGTAAAGTTTCTGCTGGAAATTTCGAGGAAGTTGCAATTGATAATGAAAAATTA
  AAAGTTCCACCATTTGCTTTTAACGGTCGTAAACCTAGCGAATGTATAGCACTAAAAATA
  AACGGAGATAGCATGAATAAAATACTCGCTAACGGTTCTTATATAATTGTCCATGATTAT
  AGAAAGTCTTGTGATCATAAACTTAACAGCAATGACATCCTTGTATTACGTCTAGGTGGT
  GAATATACAGTTAAGCGTGTGAGACGTACTGAAACAAAACTACATTTAGACCCAGCAAGC
  TATTCAGATGAATTTAAAACTAATTCTTACGATTTAGATTCTATTGATGAAATCGAAGTG
  ATAGGCAAAGTTATTTATAACTATCGTATTTTTGATTAA
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⊟Protein

⊟General

• locus tag: NWMN_1812 [new locus tag: NWMN_RS10410 ]
• symbol: NWMN_1812
• description: phage repressor
• length: 212
• theoretical pI: 5.8325
• theoretical MW: 24241.6
• GRAVY: -0.39717

⊟Function

• TIGRFAM:
  Genetic information processing DNA metabolism DNA replication, recombination, and repair repressor LexA (TIGR00498; EC 3.4.21.88; HMM-score: 40.9)
  Signal transduction Regulatory functions DNA interactions repressor LexA (TIGR00498; EC 3.4.21.88; HMM-score: 40.9)
  and 6 more
  Signal transduction Regulatory functions DNA interactions transcriptional regulator, y4mF family (TIGR03070; HMM-score: 22.8)
  Genetic information processing Protein fate Protein and peptide secretion and trafficking signal peptidase I (TIGR02227; EC 3.4.21.89; HMM-score: 20.4)
  Hypothetical proteins Conserved TIGR00270 family protein (TIGR00270; HMM-score: 19.7)
  putative zinc finger/helix-turn-helix protein, YgiT family (TIGR03830; HMM-score: 18.9)
  Cellular processes Cellular processes Detoxification nickel-type superoxide dismutase maturation protease (TIGR02754; EC 3.4.21.-; HMM-score: 13.1)
  Genetic information processing Protein fate Protein modification and repair nickel-type superoxide dismutase maturation protease (TIGR02754; EC 3.4.21.-; HMM-score: 13.1)
• TheSEED:
• PFAM:
  HTH (CL0123) HTH_3; Helix-turn-helix (PF01381; HMM-score: 49.2)
  and 8 more
  HTH_19; Helix-turn-helix domain (PF12844; HMM-score: 35.9)
  Peptidase_SF (CL0299) Peptidase_S24; Peptidase S24-like (PF00717; HMM-score: 34.9)
  HTH (CL0123) HTH_31; Helix-turn-helix domain (PF13560; HMM-score: 26.4)
  YdaS_antitoxin; Putative antitoxin of bacterial toxin-antitoxin system, YdaS/YdaT (PF15943; HMM-score: 15)
  HTH_26; Cro/C1-type HTH DNA-binding domain (PF13443; HMM-score: 14.2)
  Sigma70_r4; Sigma-70, region 4 (PF04545; HMM-score: 12.8)
  MerR; MerR family regulatory protein (PF00376; HMM-score: 12.5)
  HTH_24; Winged helix-turn-helix DNA-binding (PF13412; HMM-score: 12.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.005335
  • TAT(Tat/SPI): 0.000248
  • LIPO(Sec/SPII): 0.000412
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151222024 NCBI
• RefSeq: YP_001332846 NCBI
• UniProt: A0A0H3K9C9 UniProt

⊟Protein sequence

• MAFKNSIKEIRLNNRLSKVEMAKKLDVSEGTIRMWESGRTEPRMGMVEKISSLFNVSKGYLLGEIEEIVLPEFDSEIEVPYFGKVSAGNFEEVAIDNEKLKVPPFAFNGRKPSECIALKINGDSMNKILANGSYIIVHDYRKSCDHKLNSNDILVLRLGGEYTVKRVRRTETKLHLDPASYSDEFKTNSYDLDSIDEIEVIGKVIYNYRIFD

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: NWMN_1812 > NWMN_1813

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications