PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_1884 [new locus tag: NWMN_RS10840 ]
• pan locus tag^?: SAUPAN005041000
• symbol: NWMN_1884
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_1884 [new locus tag: NWMN_RS10840 ]
• symbol: NWMN_1884
• product: hypothetical protein
• replicon: chromosome
• strand: -
• coordinates: 2095391..2095765
• length: 375
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5332466 NCBI
• RefSeq: YP_001332918 NCBI
• BioCyc:
• MicrobesOnline: 3707472 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
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  301
  361
  
  TTGGATGATATTCAGAAAATAAAAAAAGAGCTTTCTGAATTAGTTGAACGTGTGGATGAT
  GTTGAAATACTAGCAAATGAAACAGCTGATCATGTGCTTGAACTTAGAGAGGAACATAAG
  CAACATCATAATGAACTAAGAGAATCTCATAAAGAACTTAAAGATAAGCAAGATAAAGTT
  GTAGATGAGAATTTAGAGCAAACAAAGATATTAAACAGAATTGAAGAAAGATATCAAACG
  CAAGTAGATGTTGCGCAAAAAAACGAAGAAAAGACACTCGCCCAAAATAAATGGCTCGTA
  GGTGCCATATGGGCGCTTGTAACAATTGTTATGATTGCAGTCATTACTGCATCAATTACT
  GCGTTATTACCTTAA
  60
  120
  180
  240
  300
  360
  375
  

⊟Protein

⊟General

• locus tag: NWMN_1884 [new locus tag: NWMN_RS10840 ]
• symbol: NWMN_1884
• description: hypothetical protein
• length: 124
• theoretical pI: 4.77438
• theoretical MW: 14429.3
• GRAVY: -0.557258

⊟Function

• TIGRFAM:
  SH3 domain protein (TIGR04211; HMM-score: 17)
  and 5 more
  Cellular processes Cellular processes Cell division chromosome segregation protein SMC (TIGR02168; HMM-score: 12.5)
  Genetic information processing DNA metabolism Chromosome-associated proteins chromosome segregation protein SMC (TIGR02168; HMM-score: 12.5)
  Cellular processes Cellular processes Cell division chromosome segregation protein SMC (TIGR02169; HMM-score: 9.9)
  Genetic information processing DNA metabolism Chromosome-associated proteins chromosome segregation protein SMC (TIGR02169; HMM-score: 9.9)
  Genetic information processing Protein synthesis tRNA aminoacylation serine--tRNA ligase (TIGR00414; EC 6.1.1.11; HMM-score: 9)
• TheSEED: data available for N315
• PFAM:
  no clan defined PHA_synth_III_E; Poly(R)-hydroxyalkanoic acid synthase subunit (PHA_synth_III_E) (PF09712; HMM-score: 18.4)
  CheZ; Chemotaxis phosphatase, CheZ (PF04344; HMM-score: 16.6)
  and 15 more
  Ion_channel (CL0030) Lig_chan; Ligand-gated ion channel (PF00060; HMM-score: 14.4)
  no clan defined Spt5_N; Spt5 transcription elongation factor, acidic N-terminal (PF11942; HMM-score: 14.3)
  US6; Viral unique short region 6 (PF17616; HMM-score: 13.4)
  YtxH; YtxH-like protein (PF12732; HMM-score: 13.3)
  DAG1; Dystroglycan (Dystrophin-associated glycoprotein 1) (PF05454; HMM-score: 12.8)
  TMPIT; TMPIT-like protein (PF07851; HMM-score: 11.8)
  GST_C (CL0497) GST_C; Glutathione S-transferase, C-terminal domain (PF00043; HMM-score: 11.1)
  no clan defined DHHA1; DHHA1 domain (PF02272; HMM-score: 11)
  TPR_MLP1_2; TPR/MLP1/MLP2-like protein (PF07926; HMM-score: 10.8)
  DUF724; Protein of unknown function (DUF724) (PF05266; HMM-score: 10.7)
  zf-C4H2; Zinc finger-containing protein (PF10146; HMM-score: 9.7)
  V_ATPase_I; V-type ATPase 116kDa subunit family (PF01496; HMM-score: 8.6)
  YjbJ-CsbD-like (CL0406) DUF883; Bacterial protein of unknown function (DUF883) (PF05957; HMM-score: 8.1)
  no clan defined Fib_alpha; Fibrinogen alpha/beta chain family (PF08702; HMM-score: 6.3)
  DUF1664; Protein of unknown function (DUF1664) (PF07889; HMM-score: 6.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helix: 1
• LocateP: Intracellular /TMH start AFTER 60
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: Possibly Sec-
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.001774
  • TAT(Tat/SPI): 0.001282
  • LIPO(Sec/SPII): 0.000299
• predicted transmembrane helices (TMHMM): 1

⊟Accession numbers

• GI: 151222096 NCBI
• RefSeq: YP_001332918 NCBI
• UniProt: A0A0H3K9D0 UniProt

⊟Protein sequence

• MDDIQKIKKELSELVERVDDVEILANETADHVLELREEHKQHHNELRESHKELKDKQDKVVDENLEQTKILNRIEERYQTQVDVAQKNEEKTLAQNKWLVGAIWALVTIVMIAVITASITALLP

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications