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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1933 [new locus tag: NWMN_RS11150 ]
  • pan locus tag?: SAUPAN005239000
  • symbol: NWMN_1933
  • pan gene symbol?: —
  • synonym:
  • product: acetyltransferase, GNAT family protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_1933 [new locus tag: NWMN_RS11150 ]
  • symbol: NWMN_1933
  • product: acetyltransferase, GNAT family protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2141602..2142054
  • length: 453
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    GTGGGTTATATGAGCATAATTACAAGATTGTTTAATAACAGTGATTTTGAAAAATTAAAT
    CAACTATGTAAATTATATGATGATCTAGGTTATCCAACAAATGAGAATGATTTAAAAAAG
    AGACTAAAGAAAATAACGAATCATGATGATTACTTCCTACTGCTTTTGATAAAAGAAAAT
    AAAATAATTGGTTTAAGTGGTATGTGTAAAATGATGTTTTACGAAAAAAATGCAGAGTAT
    ATGAGAATCCTTGCGTTTGTTATACATTCTGAATTTAGGAAAAAAGGTTATGGAAAGAGA
    TTATTAGCTGATTCTGAAGAATTTTCTAAACGGTTGAATTGTAAAGCAATAACACTAAAT
    AGTGGTAATAGAAATGAAAGACTATCTGCACATAAACTATATAGTGATAATGGGTATGTT
    AGCAATACATCTGGGTTTACTAAACAACTATAA
    60
    120
    180
    240
    300
    360
    420
    453

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_1933 [new locus tag: NWMN_RS11150 ]
  • symbol: NWMN_1933
  • description: acetyltransferase, GNAT family protein
  • length: 150
  • theoretical pI: 9.75545
  • theoretical MW: 17547.2
  • GRAVY: -0.547333

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification ribosomal-protein-alanine acetyltransferase (TIGR01575; EC 2.3.1.128; HMM-score: 33.6)
    and 4 more
    putative beta-lysine N-acetyltransferase (TIGR03827; EC 2.3.1.-; HMM-score: 18.8)
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan N-acetylmuramoyl-L-alanine amidase CwlD (TIGR02883; EC 3.5.1.28; HMM-score: 13.2)
    Cellular processes Cellular processes Sporulation and germination N-acetylmuramoyl-L-alanine amidase CwlD (TIGR02883; EC 3.5.1.28; HMM-score: 13.2)
    Genetic information processing Transcription DNA-dependent RNA polymerase radical SAM enzyme/protein acetyltransferase, ELP3 family (TIGR01211; EC 2.3.1.48; HMM-score: 12.1)
  • TheSEED: data available for COL, NCTC8325, USA300_FPR3757
  • PFAM:
    Acetyltrans (CL0257) Acetyltransf_1; Acetyltransferase (GNAT) family (PF00583; HMM-score: 45.2)
    and 6 more
    Acetyltransf_7; Acetyltransferase (GNAT) domain (PF13508; HMM-score: 34.1)
    Acetyltransf_10; Acetyltransferase (GNAT) domain (PF13673; HMM-score: 31.9)
    Acetyltransf_4; Acetyltransferase (GNAT) domain (PF13420; HMM-score: 18.3)
    Acetyltransf_3; Acetyltransferase (GNAT) domain (PF13302; HMM-score: 17.7)
    Acetyltransf_15; Putative acetyl-transferase (PF17013; HMM-score: 16.1)
    DUF3749; Acetyltransferase (GNAT) domain (PF12568; HMM-score: 11.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.32
    • Cytoplasmic Membrane Score: 9.55
    • Cellwall Score: 0.12
    • Extracellular Score: 0.01
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.042263
    • TAT(Tat/SPI): 0.00018
    • LIPO(Sec/SPII): 0.003742
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MGYMSIITRLFNNSDFEKLNQLCKLYDDLGYPTNENDLKKRLKKITNHDDYFLLLLIKENKIIGLSGMCKMMFYEKNAEYMRILAFVIHSEFRKKGYGKRLLADSEEFSKRLNCKAITLNSGNRNERLSAHKLYSDNGYVSNTSGFTKQL

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: SigB (activation) regulon
    SigB(sigma factor)controlling a large regulon involved in stress/starvation response and adaptation;  [1]   other strains

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Markus Bischoff, Paul Dunman, Jan Kormanec, Daphne Macapagal, Ellen Murphy, William Mounts, Brigitte Berger-Bächi, Steven Projan
    Microarray-based analysis of the Staphylococcus aureus sigmaB regulon.
    J Bacteriol: 2004, 186(13);4085-99
    [PubMed:15205410] [WorldCat.org] [DOI] (P p)

⊟Relevant publications[edit | edit source]

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