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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1934 [new locus tag: NWMN_RS11155 ]
  • pan locus tag?: SAUPAN005240000
  • symbol: NWMN_1934
  • pan gene symbol?: —
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_1934 [new locus tag: NWMN_RS11155 ]
  • symbol: NWMN_1934
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2142151..2142588
  • length: 438
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    TTGTTTATTTCTCTAGCTATTTGGTCAATAAATTCGATGGGTAATAAACAATGCATTAGT
    TTGAATTTATTGCTTTCGGATGATACTTTCGCAGTAATTTCAGTAATGACAACTTTGGGA
    ACTGCTATTTTAGGAGGTATAGCACCTTCAATATATGGTATTTTTACATCGCCTATAGAT
    AAAAAAAGTAGTTTTAGGGCTATTAGCGATTGGGAGCAAGAACAAAGAAATAATGTTAAA
    GATCTGATGATGCTATTACCATTTACATTACTTAGTATTTTAATTGTGATTTTTATTTTT
    CAGTTTGCATATTCTAAACTATTTAATACGATAGCTATTATAATATTTATTATAATACTT
    GTTGTTATGTTTTTTTTAATTTTATTAATCTGTTATAAAATTACTGTGAAGATATGCAGA
    TATTTTTCAAAAGAATAG
    60
    120
    180
    240
    300
    360
    420
    438

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_1934 [new locus tag: NWMN_RS11155 ]
  • symbol: NWMN_1934
  • description: hypothetical protein
  • length: 145
  • theoretical pI: 8.84397
  • theoretical MW: 16481.8
  • GRAVY: 0.995862

⊟Function[edit | edit source]

  • TIGRFAM:
    MSEP-CTERM protein (TIGR04286; HMM-score: 10.2)
  • TheSEED: data available for NCTC8325
  • PFAM:
    no clan defined TRP; Transient receptor potential (TRP) ion channel (PF06011; HMM-score: 12.9)
    EIID-AGA; PTS system mannose/fructose/sorbose family IID component (PF03613; HMM-score: 12.8)
    NPCC; Nuclear pore complex component (PF08058; HMM-score: 12.6)
    Yip1 (CL0112) DUF1129; Protein of unknown function (DUF1129) (PF06570; HMM-score: 10.8)
    and 4 more
    no clan defined Virul_fac_BrkB; Virulence factor BrkB (PF03631; HMM-score: 10.3)
    DUF3043; Protein of unknown function (DUF3043) (PF11241; HMM-score: 9.9)
    Ac76; Orf76 (Ac76) (PF05814; HMM-score: 9.2)
    DUF2207; Predicted membrane protein (DUF2207) (PF09972; HMM-score: 6.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 3
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0
    • Signal peptide possibility: 0
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.039755
    • TAT(Tat/SPI): 0.001271
    • LIPO(Sec/SPII): 0.002709
  • predicted transmembrane helices (TMHMM): 3

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MFISLAIWSINSMGNKQCISLNLLLSDDTFAVISVMTTLGTAILGGIAPSIYGIFTSPIDKKSSFRAISDWEQEQRNNVKDLMMLLPFTLLSILIVIFIFQFAYSKLFNTIAIIIFIIILVVMFFLILLICYKITVKICRYFSKE

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: SigB (activation) regulon
    SigB(sigma factor)controlling a large regulon involved in stress/starvation response and adaptation;  [1]   other strains

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Markus Bischoff, Paul Dunman, Jan Kormanec, Daphne Macapagal, Ellen Murphy, William Mounts, Brigitte Berger-Bächi, Steven Projan
    Microarray-based analysis of the Staphylococcus aureus sigmaB regulon.
    J Bacteriol: 2004, 186(13);4085-99
    [PubMed:15205410] [WorldCat.org] [DOI] (P p)

⊟Relevant publications[edit | edit source]

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