PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_2021 [new locus tag: NWMN_RS11680 ]
• pan locus tag^?: SAUPAN005408000
• symbol: NWMN_2021
• pan gene symbol^?: prmC
• synonym:
• product: modification methylase HemK family protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_2021 [new locus tag: NWMN_RS11680 ]
• symbol: NWMN_2021
• product: modification methylase HemK family protein
• replicon: chromosome
• strand: -
• coordinates: 2237415..2238251
• length: 837
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5331233 NCBI
• RefSeq: YP_001333055 NCBI
• BioCyc:
• MicrobesOnline: 3707614 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGGTGAATTATAAAGAAAAGTTAGATGAAGCAATTCATTTAACACAACAAAAAGGGTTT
  GAACAAACACGAGCTGAATGGTTAATGTTAGATGTATTTCAATGGACGCGTACGGACTTT
  GTAGTCCACATGCATGATGATATGCCGAAAGCGATGATTATGAAGTTCGACTTAGCATTA
  CAACGTATGTTATTAGGAGAGCCTATACAGTATATAGTTGGCTTTGCCTCATTTTATGGT
  AGAACGTTTGATGTAAACTCAAATTGTTTGATACCAAGACCTGAAACTGAAGAAGTAATG
  TTGCATTTCTTACAACAGTTAGAAGATGATGCAACAATCGTAGATATCGGAACGGGTAGT
  GGTGTACTTGCAATTACTTTGAAATGTGAAAAGCCGGATTTAAATGTTATTGCTACTGAT
  ATTTCACTTGAAGCAATGAATATGGCTCGTAATAATGCTGAGAAGCATCAATCACAAATA
  CAATTTTTAACAGGGGATGCATTAAAGCCCTTAATTAATGAAGGTATCAAGTTGAACGGC
  TTGATATCTAATCCACCATATATAGATGAAAAAGATATGGTTACGATGTCTCCAACGGTT
  ACGAGATTCGAACCACATCAGGCATTGTTTGCAGATAACCATGGATATGCTATTTATGAA
  TCAATCATTGAAGATTTACCTCACGTTATGGAAAAAGGCAGCCCAGTTGTTTTTGAAATT
  GGTTACAATCAAGGTGAGGCACTTAAATCAATAATTTTAAATAAATTTCCTGACAAAAAA
  ATCGACATTATTAAAGATATAAATGGCCACGATCGAATCGTCTCATTTAAATGGTAA
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  837
  

⊟Protein

⊟General

• locus tag: NWMN_2021 [new locus tag: NWMN_RS11680 ]
• symbol: NWMN_2021
• description: modification methylase HemK family protein
• length: 278
• theoretical pI: 4.76327
• theoretical MW: 31730.3
• GRAVY: -0.191727

⊟Function

• reaction:
  EC 2.1.1.297?  ExPASy
  Peptide chain release factor N⁵-glutamine methyltransferase S-adenosyl-L-methionine + [peptide chain release factor 1 or 2]-L-glutamine = S-adenosyl-L-homocysteine + [peptide chain release factor 1 or 2]-N⁵-methyl-L-glutamine
• TIGRFAM:
  Genetic information processing Protein fate Protein modification and repair protein-(glutamine-N5) methyltransferase, release factor-specific (TIGR03534; EC 2.1.1.-; HMM-score: 259.5)
  and 20 more
  Genetic information processing Protein fate Protein modification and repair methyltransferase, HemK family (TIGR00536; HMM-score: 207)
  Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification protein-(glutamine-N5) methyltransferase, ribosomal protein L3-specific (TIGR03533; EC 2.1.1.-; HMM-score: 114.9)
  Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification putative protein-(glutamine-N5) methyltransferase, unknown substrate-specific (TIGR03704; EC 2.1.1.-; HMM-score: 71.4)
  Unknown function Enzymes of unknown specificity putative methylase (TIGR00537; HMM-score: 47.4)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin precorrin-6Y C5,15-methyltransferase (decarboxylating), CbiT subunit (TIGR02469; EC 2.1.1.132; HMM-score: 32.8)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone ubiquinone/menaquinone biosynthesis methyltransferase (TIGR01934; EC 2.1.1.-; HMM-score: 31.6)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Biotin malonyl-acyl carrier protein O-methyltransferase BioC (TIGR02072; EC 2.1.1.-; HMM-score: 28.7)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll magnesium protoporphyrin O-methyltransferase (TIGR02021; EC 2.1.1.11; HMM-score: 27.4)
  Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification ribosomal protein L11 methyltransferase (TIGR00406; EC 2.1.1.-; HMM-score: 26.8)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone 3-demethylubiquinone-9 3-O-methyltransferase (TIGR01983; EC 2.1.1.64; HMM-score: 23.4)
  Genetic information processing Protein synthesis tRNA and rRNA base modification ribosomal RNA small subunit methyltransferase A (TIGR00755; EC 2.1.1.182; HMM-score: 23)
  Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA (guanine-N(7)-)-methyltransferase (TIGR00091; EC 2.1.1.33; HMM-score: 20.7)
  Genetic information processing Protein synthesis tRNA and rRNA base modification 23S rRNA (uracil-5-)-methyltransferase RumA (TIGR00479; EC 2.1.1.-; HMM-score: 18.7)
  Genetic information processing Protein fate Protein modification and repair protein-L-isoaspartate O-methyltransferase (TIGR00080; EC 2.1.1.77; HMM-score: 15.1)
  Genetic information processing Protein synthesis tRNA and rRNA base modification 16S rRNA (guanine(527)-N(7))-methyltransferase RsmG (TIGR00138; EC 2.1.1.170; HMM-score: 13.9)
  Genetic information processing Transcription RNA processing 3' terminal RNA ribose 2'-O-methyltransferase Hen1 (TIGR04074; EC 2.1.1.-; HMM-score: 13.9)
  Genetic information processing Protein synthesis tRNA and rRNA base modification 3' terminal RNA ribose 2'-O-methyltransferase Hen1 (TIGR04074; EC 2.1.1.-; HMM-score: 13.9)
  2-ketoarginine methyltransferase (TIGR04543; EC 2.1.1.243; HMM-score: 13.2)
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides putative sugar O-methyltransferase (TIGR04371; EC 2.1.1.-; HMM-score: 11.6)
  Genetic information processing Protein synthesis tRNA and rRNA base modification 23S rRNA (uracil-5-)-methyltransferase RumB (TIGR02085; EC 2.1.1.189; HMM-score: 10.8)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  NADP_Rossmann (CL0063) MTS; Methyltransferase small domain (PF05175; HMM-score: 57.7)
  and 18 more
  Methyltransf_25; Methyltransferase domain (PF13649; HMM-score: 34.6)
  Methyltransf_18; Methyltransferase domain (PF12847; HMM-score: 34.4)
  Methyltransf_31; Methyltransferase domain (PF13847; HMM-score: 34.1)
  PrmA; Ribosomal protein L11 methyltransferase (PrmA) (PF06325; HMM-score: 31.8)
  PCMT; Protein-L-isoaspartate(D-aspartate) O-methyltransferase (PCMT) (PF01135; HMM-score: 26)
  Methyltransf_12; Methyltransferase domain (PF08242; HMM-score: 24)
  Methyltransf_4; Putative methyltransferase (PF02390; HMM-score: 23.9)
  Methyltransf_11; Methyltransferase domain (PF08241; HMM-score: 22)
  Methyltransf_23; Methyltransferase domain (PF13489; HMM-score: 21.5)
  Methyltransf_32; Methyltransferase domain (PF13679; HMM-score: 19.7)
  Methyltransf_2; O-methyltransferase (PF00891; HMM-score: 19.6)
  Methyltransf_16; Lysine methyltransferase (PF10294; HMM-score: 19.1)
  GidB; rRNA small subunit methyltransferase G (PF02527; HMM-score: 17.5)
  CmcI; Cephalosporin hydroxylase (PF04989; HMM-score: 16.8)
  Methyltransf_15; RNA cap guanine-N2 methyltransferase (PF09445; HMM-score: 16.6)
  TrmK; tRNA (adenine(22)-N(1))-methyltransferase (PF04816; HMM-score: 16.1)
  UPF0020; Putative RNA methylase family UPF0020 (PF01170; HMM-score: 13.8)
  CMAS; Mycolic acid cyclopropane synthetase (PF02353; HMM-score: 12.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.001389
  • TAT(Tat/SPI): 0.000134
  • LIPO(Sec/SPII): 0.000249
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151222233 NCBI
• RefSeq: YP_001333055 NCBI
• UniProt: A0A0H3KE45 UniProt

⊟Protein sequence

• MVNYKEKLDEAIHLTQQKGFEQTRAEWLMLDVFQWTRTDFVVHMHDDMPKAMIMKFDLALQRMLLGEPIQYIVGFASFYGRTFDVNSNCLIPRPETEEVMLHFLQQLEDDATIVDIGTGSGVLAITLKCEKPDLNVIATDISLEAMNMARNNAEKHQSQIQFLTGDALKPLINEGIKLNGLISNPPYIDEKDMVTMSPTVTRFEPHQALFADNHGYAIYESIIEDLPHVMEKGSPVVFEIGYNQGEALKSIILNKFPDKKIDIIKDINGHDRIVSFKW

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: NWMN_2019 < NWMN_2020 < NWMN_2021 < prfA < tdk

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications