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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_2026 [new locus tag: NWMN_RS11705 ]
  • pan locus tag?: SAUPAN005414000
  • symbol: NWMN_2026
  • pan gene symbol?: —
  • synonym:
  • product: aldehyde dehydrogenase family protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_2026 [new locus tag: NWMN_RS11705 ]
  • symbol: NWMN_2026
  • product: aldehyde dehydrogenase family protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2242196..2243623
  • length: 1428
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGAGAGACTACACAAAGCAATACATTAATGGCGAATGGGTAGAAAGTAATAGTAATGAA
    ACGATAGAAGTTATAAATCCAGCAACCGAAGAAGTAATCGGGAAAGTTGCTAAAGGTAAT
    AAAGCTGATGTTGATAAAGCCGTCGAGGCGGCAGACGATGTTTATTTAGAGTTCCGTCAT
    ACATCTGTGAAAGAAAGACAAGCGTTATTAGATAAAATTGTAAAAGAATATGAAAACAGA
    AAAGACGATATTGTACAAGCTATTACGGATGAATTAGGTGCTCCTTTATCATTATCTGAG
    CGTGTCCATTATCAAATGGGACTAAACCATTTTGTTGCAGCGAGAGACGCATTAGATAAC
    TACGAATTTGAAGAACGCCGCGGAGATGATTTAGTTGTTAAAGAAGCAATCGGTGTATCT
    GGATTAATTACACCGTGGAACTTCCCTACAAACCAAACATCATTAAAATTAGCAGCAGCA
    TTTGCGGCTGGTAGTCCAGTTGTACTTAAACCATCTGAAGAAACACCATTTGCAGCTGTT
    ATTTTAGCTGAGATTTTTGATAAAGTCGGTGTTCCTAAAGGTGTATTTAACCTTGTTAAT
    GGTGATGGTGCTGGTGTTGGGAATCCTTTATCTGAACATCCTAAAGTACGCATGATGTCA
    TTTACAGGATCAGGCCCTACTGGTTCTAAAATTATGGAAAAAGCCGCTAAAGATTTTAAA
    AAGGTATCATTAGAGCTTGGTGGCAAATCACCATATATCGTCCTAGATGACGTAGATATT
    AAAGAAGCGGCTAAAGCAACAACAGGCAAAGTTGTTAATAATACTGGTCAAGTATGTACA
    GCTGGTACACGTGTTTTAGTGCCTAACAAAATTAAAGATGCATTCTTAGCTGAATTAAAA
    GAACAATTTAGCCAAGTGCGTGTCGGTAATCCAAGAGAAGATGGTACACAAGTAGGCCCT
    ATCATTAGTAAAAAACAATTTGATCAAGTACAAAATTATATTAATAAAGGTATTGAAGAA
    GGTGCTGAATTATTTTATGGTGGTCCTGGTAAACCAGAAGGACTTGAAAAAGGATACTTT
    GCACGTCCGACAATTTTTATTAATGTAGATAATCAAATGACGATAGCACAAGAAGAAATT
    TTTGGGCCAGTAATGTCAGTTATCACTTATAACGATTTAGATGAAGCGATTCAAATTGCA
    AATGATACAAAATATGGTTTGGCAGGATATGTTATTGGTAAGGACAAAGAAACATTGCAT
    AAAGTAGCTCGTTCTATTGAAGCAGGTACAGTAGAAATAAACGAAGCAGGTAGAAAGCCA
    GATTTACCATTTGGTGGCTATAAACAATCTGGTTTAGGTCGTGAATGGGGCGATTATGGT
    ATTGAAGAGTTCTTAGAAGTGAAATCTATAGCTGGATATTTTAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1428

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_2026 [new locus tag: NWMN_RS11705 ]
  • symbol: NWMN_2026
  • description: aldehyde dehydrogenase family protein
  • length: 475
  • theoretical pI: 4.76962
  • theoretical MW: 51968.3
  • GRAVY: -0.350526

⊟Function[edit | edit source]

  • TIGRFAM:
    Cellular processes Cellular processes Adaptations to atypical conditions betaine-aldehyde dehydrogenase (TIGR01804; EC 1.2.1.8; HMM-score: 460)
    5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase (TIGR02299; EC 1.2.1.60; HMM-score: 449.2)
    Unknown function Enzymes of unknown specificity aldehyde dehydrogenase, Rv0768 family (TIGR04284; EC 1.2.1.-; HMM-score: 405)
    Metabolism Central intermediary metabolism Other succinate-semialdehyde dehydrogenase (TIGR01780; EC 1.2.1.-; HMM-score: 403.8)
    Metabolism Energy metabolism Other 2-hydroxymuconic semialdehyde dehydrogenase (TIGR03216; EC 1.2.1.-; HMM-score: 375.8)
    and 10 more
    Metabolism Energy metabolism Amino acids and amines putative delta-1-pyrroline-5-carboxylate dehydrogenase (TIGR01237; EC 1.2.1.88; HMM-score: 314.6)
    1-pyrroline dehydrogenase (TIGR03374; EC 1.2.1.19; HMM-score: 293.2)
    Metabolism Energy metabolism Amino acids and amines methylmalonate-semialdehyde dehydrogenase (acylating) (TIGR01722; EC 1.2.1.27; HMM-score: 274.6)
    putative phosphonoacetaldehyde dehydrogenase (TIGR03250; EC 1.2.1.-; HMM-score: 265.1)
    Metabolism Energy metabolism Amino acids and amines succinylglutamate-semialdehyde dehydrogenase (TIGR03240; EC 1.2.1.71; HMM-score: 223.8)
    Metabolism Energy metabolism Amino acids and amines delta-1-pyrroline-5-carboxylate dehydrogenase (TIGR01238; EC 1.2.1.88; HMM-score: 181.5)
    Metabolism Energy metabolism Amino acids and amines 1-pyrroline-5-carboxylate dehydrogenase (TIGR01236; EC 1.2.1.88; HMM-score: 169.2)
    phenylacetic acid degradation protein paaN (TIGR02278; HMM-score: 142.6)
    phenylacetic acid degradation protein paaN (TIGR02288; HMM-score: 48.8)
    acetaldehyde dehydrogenase (acetylating) (TIGR02518; EC 1.2.1.10; HMM-score: 23.1)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    ALDH-like (CL0099) Aldedh; Aldehyde dehydrogenase family (PF00171; HMM-score: 552.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.012993
    • TAT(Tat/SPI): 0.000365
    • LIPO(Sec/SPII): 0.000952
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MRDYTKQYINGEWVESNSNETIEVINPATEEVIGKVAKGNKADVDKAVEAADDVYLEFRHTSVKERQALLDKIVKEYENRKDDIVQAITDELGAPLSLSERVHYQMGLNHFVAARDALDNYEFEERRGDDLVVKEAIGVSGLITPWNFPTNQTSLKLAAAFAAGSPVVLKPSEETPFAAVILAEIFDKVGVPKGVFNLVNGDGAGVGNPLSEHPKVRMMSFTGSGPTGSKIMEKAAKDFKKVSLELGGKSPYIVLDDVDIKEAAKATTGKVVNNTGQVCTAGTRVLVPNKIKDAFLAELKEQFSQVRVGNPREDGTQVGPIISKKQFDQVQNYINKGIEEGAELFYGGPGKPEGLEKGYFARPTIFINVDNQMTIAQEEIFGPVMSVITYNDLDEAIQIANDTKYGLAGYVIGKDKETLHKVARSIEAGTVEINEAGRKPDLPFGGYKQSGLGREWGDYGIEEFLEVKSIAGYFK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:
    NWMN_0509(fus)elongation factor G  [1] (data from MRSA252)
    NWMN_1592(pykA)pyruvate kinase  [1] (data from MRSA252)
    NWMN_0510(tufA)elongation factor Tu  [1] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: SigB (activation) regulon
    SigB(sigma factor)controlling a large regulon involved in stress/starvation response and adaptation;  [2] [3]   other strains

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.0 1.1 1.2 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)
  2. ↑ Markus Bischoff, Paul Dunman, Jan Kormanec, Daphne Macapagal, Ellen Murphy, William Mounts, Brigitte Berger-Bächi, Steven Projan
    Microarray-based analysis of the Staphylococcus aureus sigmaB regulon.
    J Bacteriol: 2004, 186(13);4085-99
    [PubMed:15205410] [WorldCat.org] [DOI] (P p)
  3. ↑ Bettina Schulthess, Dominik A Bloes, Patrice François, Myriam Girard, Jacques Schrenzel, Markus Bischoff, Brigitte Berger-Bächi
    The σB-dependent yabJ-spoVG operon is involved in the regulation of extracellular nuclease, lipase, and protease expression in Staphylococcus aureus.
    J Bacteriol: 2011, 193(18);4954-62
    [PubMed:21725011] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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