PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_2085
• pan locus tag^?: SAUPAN005576000
• symbol: NWMN_2085
• pan gene symbol^?: —
• synonym:
• product: putative transposase for IS1272

⊟Additional information (user-provided)

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_2085
• symbol: NWMN_2085
• product: putative transposase for IS1272
• replicon: chromosome
• strand: +
• coordinates: 2323433..2323792
• length: 360
• essential: unknown

⊟Accession numbers

• Gene ID: 5331277 NCBI
• RefSeq: YP_001333119 NCBI
• BioCyc:
• MicrobesOnline: 3707687 MicrobesOnline

⊟Phenotype

⊟Additional information (user-provided)

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  
  TTGAGACATCAATGCATGAAACCAAATTCGAAATCCAATAAGAAAATCATGAAGAATTAT
  AATTGGGAATACTTTAAAGTCCAAATTAATCAAAAGCTTTCTGAACCAGAAACGAAAAAA
  ATCTATAGTCAAAGAAAAATTGATGTAGAGCCTGTTTTTGGATTTATGAAGGCTATTTTG
  GGTTTCACTCGAATGTCAGTTCGAGGAATAAATAAAGTTAAACGAGAGCTAGGTTTTGTA
  TTAATGGCACTTAATATAAGGAAAATAGCAGCTCAACGAGCTGTACATTATAAAATACAT
  ATCAAAAAAGCTGATTTCTATCAAATAATTAATAGAAATCAGCTTTTTTACATTGCCTAA
  60
  120
  180
  240
  300
  360
  

⊟Protein

⊟General

• locus tag: NWMN_2085
• symbol: NWMN_2085
• description: putative transposase for IS1272
• length: 119
• theoretical pI: 11.0008
• theoretical MW: 14241.9
• GRAVY: -0.471429

⊟Function

• TIGRFAM:
• TheSEED  :
  • Mobile element protein
• PFAM:
  RNase_H (CL0219) DDE_Tnp_1_6; Transposase DDE domain (PF13751; HMM-score: 61)
  and 1 more
  DDE_Tnp_1; Transposase DDE domain (PF01609; HMM-score: 41.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0.32
  • Cytoplasmic Membrane Score: 9.55
  • Cellwall Score: 0.12
  • Extracellular Score: 0.01
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.8615
  • Cytoplasmic Membrane Score: 0.0004
  • Cell wall & surface Score: 0.0012
  • Extracellular Score: 0.137
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.00969
  • TAT(Tat/SPI): 0.000371
  • LIPO(Sec/SPII): 0.000842
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151222297 NCBI
• RefSeq: YP_001333119 NCBI
• UniProt: A0A0H3K9W4 UniProt

⊟Additional information (user-provided)

⊟Protein sequence

• MRHQCMKPNSKSNKKIMKNYNWEYFKVQINQKLSEPETKKIYSQRKIDVEPVFGFMKAILGFTRMSVRGINKVKRELGFVLMALNIRKIAAQRAVHYKIHIKKADFYQIINRNQLFYIA

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Additional information (user-provided)

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Additional information (user-provided)

⊟Other information (user-provided)

You can add further information about the gene and protein here. [edit]

⊟Literature

⊟References

⊟Relevant publications