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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_2186 [new locus tag: NWMN_RS12620 ]
  • pan locus tag?: SAUPAN005749000
  • symbol: NWMN_2186
  • pan gene symbol?: ydbM
  • synonym:
  • product: acyl-CoA dehydrogenase-related protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_2186 [new locus tag: NWMN_RS12620 ]
  • symbol: NWMN_2186
  • product: acyl-CoA dehydrogenase-related protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2407441..2408376
  • length: 936
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGTTATTTTGCAATCATTTTTAGGCGAACTTGATGGTGCCACAGCATTATCTATTGGT
    TGGCATGTGAGTGTCGTAGGACAAATTTATGAACAGAAATTATGGTCTCAAGATATGTTG
    GAGCAATTTGCTGTTGAAATTAATAATGGTGCATTAGTTAATAGAGCAGTTAGTGAAGCT
    GAAATGGGTAGTCCAACAAGAGGGGGAAGACCAAGTACACATGCTGTTAAAGCTGATGAT
    GGGTATATTTTAAATGGTGTGAAGACATATACATCAATGAGTAAAGCACTAACACATATT
    ATTGTTGCTGCTTATATAGAAGAATTAGAAAGTGTTGGTTTTTTCTTAGTAGACAGGAAT
    TTGCCTGGTGTTGAAATTGCCGATAATTGGGACGTATTAGGTATGCGAGCGACAGAAAGT
    CATGATTTAATATTAAATGATGTAAAAGTACCATTAAAACATTTAGTGGAAACAGAGAAA
    AGTAAAGCGCCAAATGGTTGGATATTGCATATACCAAGCTGTTATTTAGGTATAGCACAA
    GCTGCTAGAAATTATGCTGTAGACTTTGCAATCCAACATAGCCCTAATAGTATTGAAGGA
    ACGATTGCAACATTGCCAACTGTGCAACAAAATTTAGGGAAAATGGAAACGCTATTGTTA
    TGTGCGAGACAATTTTTATGGAGTACAGCAAAAGGGTATCAACAATATAAAGATGACAGT
    CAGATAAGAAATCCAACAAGTGCGAGTAAAGTGATGGTAATGAATCAAGGGCTTGAAGTG
    ATTGATTTAGCGATGAGAATAGTTGGTGCTAAAAGCTTAGAAATGAATCGACCATTACAA
    CGTTACTATCGTGACATGCGTGCAGGATTACACAATCCACCTATGGAAGATGCAGCATAT
    ACAAATATTGCTAAGAGTATTACAGATACATTTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    936

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_2186 [new locus tag: NWMN_RS12620 ]
  • symbol: NWMN_2186
  • description: acyl-CoA dehydrogenase-related protein
  • length: 311
  • theoretical pI: 5.85695
  • theoretical MW: 34413.1
  • GRAVY: -0.117685

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Central intermediary metabolism Sulfur metabolism sulfur acquisition oxidoreductase, SfnB family (TIGR04022; EC 1.-.-.-; HMM-score: 73)
    and 2 more
    cyclohexanecarboxyl-CoA dehydrogenase (TIGR03207; EC 1.1.1.-; HMM-score: 30.9)
    pimeloyl-CoA dehydrogenase, large subunit (TIGR03204; EC 1.3.1.62; HMM-score: 21.5)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    Acyl-CoA_dh (CL0087) Acyl-CoA_dh_2; Acyl-CoA dehydrogenase, C-terminal domain (PF08028; HMM-score: 51.7)
    no clan defined Acyl-CoA_dh_M; Acyl-CoA dehydrogenase, middle domain (PF02770; HMM-score: 50.6)
    Acyl-CoA_dh (CL0087) Acyl-CoA_dh_1; Acyl-CoA dehydrogenase, C-terminal domain (PF00441; HMM-score: 45.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.009374
    • TAT(Tat/SPI): 0.000818
    • LIPO(Sec/SPII): 0.001339
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MVILQSFLGELDGATALSIGWHVSVVGQIYEQKLWSQDMLEQFAVEINNGALVNRAVSEAEMGSPTRGGRPSTHAVKADDGYILNGVKTYTSMSKALTHIIVAAYIEELESVGFFLVDRNLPGVEIADNWDVLGMRATESHDLILNDVKVPLKHLVETEKSKAPNGWILHIPSCYLGIAQAARNYAVDFAIQHSPNSIEGTIATLPTVQQNLGKMETLLLCARQFLWSTAKGYQQYKDDSQIRNPTSASKVMVMNQGLEVIDLAMRIVGAKSLEMNRPLQRYYRDMRAGLHNPPMEDAAYTNIAKSITDTF

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: CymR* (repression) regulon, SigB (activation) regulon
    CymR*(TF)important in Cysteine metabolism; RegPrecise 
    SigB(sigma factor)controlling a large regulon involved in stress/starvation response and adaptation;  [1]   other strains

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Bettina Schulthess, Dominik A Bloes, Patrice François, Myriam Girard, Jacques Schrenzel, Markus Bischoff, Brigitte Berger-Bächi
    The σB-dependent yabJ-spoVG operon is involved in the regulation of extracellular nuclease, lipase, and protease expression in Staphylococcus aureus.
    J Bacteriol: 2011, 193(18);4954-62
    [PubMed:21725011] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

Olga Soutourina, Sarah Dubrac, Olivier Poupel, Tarek Msadek, Isabelle Martin-Verstraete
The pleiotropic CymR regulator of Staphylococcus aureus plays an important role in virulence and stress response.
PLoS Pathog: 2010, 6(5);e1000894
[PubMed:20485570] [WorldCat.org] [DOI] (I e)

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