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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_2201 [new locus tag: NWMN_RS12705 ]
  • pan locus tag?: SAUPAN005770000
  • symbol: NWMN_2201
  • pan gene symbol?: —
  • synonym:
  • product: dehydrogenase family protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_2201 [new locus tag: NWMN_RS12705 ]
  • symbol: NWMN_2201
  • product: dehydrogenase family protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2422444..2423526
  • length: 1083
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGAAAATAGCAATTGTAGGATCAGGAAATGGCGCAGTTACGGCAGCAGTAGATATGGTG
    AGCAAAGGCCACGATGTTAAATTATATTGTCGTAATCAATCTATAAGTAAGTTTCAAAAC
    GCAATCGAAAAGGGCGGATTTGATTTTAATAATGAAGGTGATGAACGTTTCGTAAAATTC
    ACTGATATTAGTGATGATATGGAATATGTTTTAAAAGATGCTGAAATTGTTCAAGTGATT
    ATTCCATCTTCATACATAGAGTATTATGCTGATGTAATGGCAGAGCATGTAACTGATAAT
    CAGTTGATATTCTTCAACATGGCTGCAGCAATGGGGTCAATTCGTTTTATGAATGTTTTA
    GAAGATAGACATATTGAAACAAAACCACAACTAGCGGAAGCTAATACGTTGACGTATGGT
    ACGCGTGTCGATTTTGAAAATGCAGCAGTTGATTTATCTCTAAATGTACGTCGTATCTTC
    TTTTCAACATATGATAGAAGCTGTCTAAATGATTGTTATGACAAAGTTTCAAGTATTTAT
    GATCATTTAGTAAAAGAGGAAAGCTTAATTAAAACAAATCTTGAAAATGGTAATCCTGAA
    GTGCATCCAGGACCAACATTATTGAATGTCGGTCGCATTGATTATGCTGGCGAGTTCGCT
    TTATATAAAGAAGGAATTACTAAACATACAGTTAGATTACTTCATGCAATCGAATTAGAA
    CGTTTGAATTTAGGCCGTAGATTAGGTTTTGAATTATCAACAGCTAAAGAATCACGTATT
    GAACGTGGTTATTTAGAACGTGATAAAGAAGATGAACCATTAAATCGTTTGTTTAATACA
    AGCCCAGTATTTTCACAAATTCCAGGACCAAATCATGTAGAAAGCAGATATTTAACTGAA
    GATATTGCATATGGTTTAGTACTATGGTCAAGCTTAGGTCGTGTTATTGATGTACCGACA
    CCAAATATAGATGCAGTAATTGTAATTGCATCAACCATTTTAGAGAGAGACTTCTTTGAG
    GAAGGCTTAACAGTTGAAGAAATTGGTTTAGATAAGCTTGATTTAGAAAAATATTTAAAA
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1083

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_2201 [new locus tag: NWMN_RS12705 ]
  • symbol: NWMN_2201
  • description: dehydrogenase family protein
  • length: 360
  • theoretical pI: 4.56718
  • theoretical MW: 40741.8
  • GRAVY: -0.2325

⊟Function[edit | edit source]

  • TIGRFAM:
    9,9'-di-cis-zeta-carotene desaturase (TIGR02732; EC 1.3.5.6; HMM-score: 17.6)
    Unknown function Enzymes of unknown specificity putative bacillithiol system oxidoreductase, YpdA family (TIGR04018; EC 1.8.-.-; HMM-score: 16.8)
    and 2 more
    Metabolism Energy metabolism TCA cycle succinate dehydrogenase or fumarate reductase, flavoprotein subunit (TIGR01811; EC 1.3.99.1; HMM-score: 11.3)
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA U-34 5-methylaminomethyl-2-thiouridine biosynthesis protein MnmC, C-terminal domain (TIGR03197; HMM-score: 10.9)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    6PGD_C (CL0106) Octopine_DH; NAD/NADP octopine/nopaline dehydrogenase, alpha-helical domain (PF02317; HMM-score: 125.9)
    and 6 more
    NADP_Rossmann (CL0063) NAD_Gly3P_dh_N; NAD-dependent glycerol-3-phosphate dehydrogenase N-terminus (PF01210; HMM-score: 22)
    Pyr_redox_3; Pyridine nucleotide-disulphide oxidoreductase (PF13738; HMM-score: 20.4)
    ApbA; Ketopantoate reductase PanE/ApbA (PF02558; HMM-score: 14.1)
    Pyr_redox_2; Pyridine nucleotide-disulphide oxidoreductase (PF07992; HMM-score: 14)
    FAD_binding_2; FAD binding domain (PF00890; HMM-score: 10.6)
    IlvN; Acetohydroxy acid isomeroreductase, NADPH-binding domain (PF07991; HMM-score: 10.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.052737
    • TAT(Tat/SPI): 0.000669
    • LIPO(Sec/SPII): 0.013352
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKIAIVGSGNGAVTAAVDMVSKGHDVKLYCRNQSISKFQNAIEKGGFDFNNEGDERFVKFTDISDDMEYVLKDAEIVQVIIPSSYIEYYADVMAEHVTDNQLIFFNMAAAMGSIRFMNVLEDRHIETKPQLAEANTLTYGTRVDFENAAVDLSLNVRRIFFSTYDRSCLNDCYDKVSSIYDHLVKEESLIKTNLENGNPEVHPGPTLLNVGRIDYAGEFALYKEGITKHTVRLLHAIELERLNLGRRLGFELSTAKESRIERGYLERDKEDEPLNRLFNTSPVFSQIPGPNHVESRYLTEDIAYGLVLWSSLGRVIDVPTPNIDAVIVIASTILERDFFEEGLTVEEIGLDKLDLEKYLK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: SigB (activation) regulon
    SigB(sigma factor)controlling a large regulon involved in stress/starvation response and adaptation;  [1]   other strains

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Bettina Schulthess, Dominik A Bloes, Patrice François, Myriam Girard, Jacques Schrenzel, Markus Bischoff, Brigitte Berger-Bächi
    The σB-dependent yabJ-spoVG operon is involved in the regulation of extracellular nuclease, lipase, and protease expression in Staphylococcus aureus.
    J Bacteriol: 2011, 193(18);4954-62
    [PubMed:21725011] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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