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NCBI: 06-JUL-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_2284 [new locus tag: NWMN_RS13145 ]
  • pan locus tag?: SAUPAN005911000
  • symbol: NWMN_2284
  • pan gene symbol?: gltT
  • synonym:
  • product: proton/sodium-glutamate symporter

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_2284 [new locus tag: NWMN_RS13145 ]
  • symbol: NWMN_2284
  • product: proton/sodium-glutamate symporter
  • replicon: chromosome
  • strand: -
  • coordinates: 2509881..2511158
  • length: 1278
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGCTCTATTCAAGAGAAAAATTAGCCTACCTATGCAAGTTGTTATTGCATTAGTGTTA
    GGTGTTGTCGTAGGACTTTTATTATATGGACAAGAAAACGTAGCAAATTACATTAAACCA
    TTTGGTGACGTATTTTTAAATTTAATTAAAATGATCGTTATACCAGTTGTATTTTGCTCA
    CTAGCGCTTTCTATTTCGAACGTTGGGGAATCGAAAACTGTAGGGCGTTATGGCTGGAAA
    ACAATTTTATACTTTGAAATTATTACAACAATCGCAATAGGTTTAGGGATTATCTTCGGT
    AACCTATTTAAACCAGGTGCTGGATTAGACCCAACAAAATTACCTAAAGGTGATATTTCT
    AAATATCAATCAACTGCACATGCAGCAGAACAATCTACATATGGAAATCATTTTATTGAT
    ACCATTGTACATATTATTCCGACAAACTTTTTTGAAGCTTTAAATAAGGGTGAATTATTA
    CCTATTATCTTCTTCGCAGTATTCTTTGGATTAGGATTAGCTGCTGTAGGTAAAAAAGCA
    GAACCAGTTAAAGAATTTTTAAGCGGATCGCTTGAAGCTGTGTTCTGGATGATTAATAAA
    ATTTTAAAATTAGCACCACTTGGAGTGTTTGCATTCATTTGTACTACAATTATTACATTT
    GGTGCATCCGCATTATTACCACTATTAAAATTAGTATTAGTTGTTGTCTTTGCAATGGTG
    TTCTTTGTATTCGCTATACTAGGACTAGTTGCATGGATGTGTGTTATTAATATCATGAAT
    ATTATTAGAATCTTGAAAAGTGAATTGCTTTTAGCATTTTCTACATCAAGTTCGGAAGCT
    GTACTTCCTGTAATGATGAAGAAAATGGAAAACTTCGGTTCTCCAAAAGAAATTACTTCT
    TTTGTTATACCAATTGGTTATACGTTTAACTTAGATGGATCAGCACTTTATCAATCTATT
    GCAGCATTATTCGTTGCACAGATGTATGGAATGCACTTAACATTATCAGAGCAAATTGTG
    TTGATGTTAACATTAATGATTACGTCTAAAGGTATGGCGGCAGTACCAGGAACTTCAATC
    GTTGTTTTATTAACAACATTAGGTGCCATGGGCTTACCGGCACAAGGTTTAGCATTAATT
    ATTGGTGTTGACCGTATCTTAGATATGGTACGTACATGTGTAAACGTTATTGGTAATGCA
    TTATCAACAATCGTTATAGCTAAATGGGAAAACGTATATGACAAAGCAAAAGGTCAAGAA
    TATTTAAAATCAATTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1278

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: NWMN_2284 [new locus tag: NWMN_RS13145 ]
  • symbol: NWMN_2284
  • description: proton/sodium-glutamate symporter
  • length: 425
  • theoretical pI: 9.36462
  • theoretical MW: 46110.2
  • GRAVY: 0.884941

Function[edit | edit source]

  • TIGRFAM:
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    no clan defined SDF; Sodium:dicarboxylate symporter family (PF00375; HMM-score: 406.7)
    and 1 more
    DUF2701; Protein of unknown function (DUF2701) (PF10857; HMM-score: 8.7)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 7
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 0
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.050823
    • TAT(Tat/SPI): 0.000753
    • LIPO(Sec/SPII): 0.045106
  • predicted transmembrane helices (TMHMM): 8

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MALFKRKISLPMQVVIALVLGVVVGLLLYGQENVANYIKPFGDVFLNLIKMIVIPVVFCSLALSISNVGESKTVGRYGWKTILYFEIITTIAIGLGIIFGNLFKPGAGLDPTKLPKGDISKYQSTAHAAEQSTYGNHFIDTIVHIIPTNFFEALNKGELLPIIFFAVFFGLGLAAVGKKAEPVKEFLSGSLEAVFWMINKILKLAPLGVFAFICTTIITFGASALLPLLKLVLVVVFAMVFFVFAILGLVAWMCVINIMNIIRILKSELLLAFSTSSSEAVLPVMMKKMENFGSPKEITSFVIPIGYTFNLDGSALYQSIAALFVAQMYGMHLTLSEQIVLMLTLMITSKGMAAVPGTSIVVLLTTLGAMGLPAQGLALIIGVDRILDMVRTCVNVIGNALSTIVIAKWENVYDKAKGQEYLKSI

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]