PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_2370 [new locus tag: NWMN_RS13655 ]
• pan locus tag^?: SAUPAN006037000
• symbol: NWMN_2370
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_2370 [new locus tag: NWMN_RS13655 ]
• symbol: NWMN_2370
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 2607919..2609457
• length: 1539
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5331452 NCBI
• RefSeq: YP_001333404 NCBI
• BioCyc:
• MicrobesOnline: 3707972 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  1381
  1441
  1501
  
  ATGACATCAAAACATCAACAAAAGGGGTCTATCGTCAATCGTTTCTTGAATAGTGTTGAA
  AAAATCGGAAATAAATTGCCAGATCCTAGCGTCTTATTCTTTTTAATGTGTGTGGGCTTA
  GCCATTATGACATGGGTTATCTCATTATTTAATGTATCTGTTAAGCATCCAGGTACGCAT
  CAAACCATTTATATTAAAAATATAATTAGCCATGATGGATTTACGATGATAATGAATGAT
  ACGATTAAAAATTTCTCAGAGTTCCCAGCATTAGGCTTAGTACTAGCAGTGATGATTGGT
  ATTGGTGTTGCAGAGAAAACAGGATACTTCGATAAGTTAATGATTTCTGTTGTGAATCGC
  GCACCACGTTTCTTAATTTTACCGACTATTATATTAATTGGTATTTTAGGTAGTACAGCC
  GGCGATGCTGCGACAATTATCTTGCCGCCGCTTGCAGCAATGCTTTTTATTAAAATTGGC
  TATCACCCTATCGCTGGACTAACGATGGCATATGCTTCCGCTGTTGGAGGATTTGCAGCA
  AATATAGTTGTTGGTATGCAAGATGCTTTGGTCTATTCATTTACAGAACCGGCAACACGT
  ATCGTTTCAGATTCTATTAAAACAAACGTTGCTATGAACTGGTACTTTATCGCAGCGAGT
  GTCGTTGTATTACTTCCTACGATATTGCTAGTTACAACTAAGCTCATTATTCCTAGATTA
  GGAAAATATGATGACAGTTTAATGCATGATGACCATGAAGAAACATCTTCACATATTACT
  GACAAAGAAGCACATGCTTTAAAATGGGCAAATATCAGTTTCATAGTCACAATTATTTTA
  TTAATTATTACAGCCATTCCTGAACATAGCTTTTTAAGAAATGCTAAAACAGGCAGCTTA
  CTAGACGATGCGCCATTAATTAACGGTGTCGGATTAATTATTCTTGTCGTATTTTTAGTA
  CCTGGATTAGTTTATGGGATTTTAAGCAAAGAAATTAAAAATACAAAAGACTTAGGTAAA
  ATGTTCGGCGATGCTGTAGGATCAATGGGAACATTTATCGTTATTGTGTTTTTTGCAGCA
  CAGCTACTTGCGTATTTAAAGTGGAGTAATTTAGGAATTATCGCTGCTGTTAAAGGTGCC
  AAATTATTAGAACATCAAAACGGTATTGTATTGATACTAGGTATTATTGTACTGAGTGCA
  ATGGTCAACATGTTAATCGGAAGCGCATCGGCTAAATGGGGTATTTTAGGACCGATATTC
  GTACCAATGTTAATCCTTATCGGCTTTCATCCCGCATTCACACAAGTCATTTACCGTGTA
  GGCGATTCAATTACCAATCCAATTACACCGATGATGCCGTACTTACCTTTATTATTAACT
  TATGCACAAAAATACGATAAACGCATGAAACTCGGAGCCTTACTTTCTAGTTTAATGCCG
  TATTCAATCGCGCTAAGTATTGTATGGACAGTATTTGTCATCATTTGGTTCCTATTAGGT
  ATCCCTGTTGGACCAGGTGGACCGATATTCGTTAAATAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1140
  1200
  1260
  1320
  1380
  1440
  1500
  1539
  

⊟Protein

⊟General

• locus tag: NWMN_2370 [new locus tag: NWMN_RS13655 ]
• symbol: NWMN_2370
• description: hypothetical protein
• length: 512
• theoretical pI: 9.7071
• theoretical MW: 55574.3
• GRAVY: 0.775391

⊟Function

• TIGRFAM:
  Hypothetical proteins Conserved AbgT transporter family (TIGR00819; HMM-score: 447.5)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  IT (CL0182) ABG_transport; AbgT putative transporter family (PF03806; HMM-score: 726.3)
  and 2 more
  DcuC; C4-dicarboxylate anaerobic carrier (PF03606; HMM-score: 26.3)
  Na_H_antiporter; Na+/H+ antiporter family (PF03553; HMM-score: 25.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 11
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.004399
  • TAT(Tat/SPI): 0.000242
  • LIPO(Sec/SPII): 0.001808
• predicted transmembrane helices (TMHMM): 12

⊟Accession numbers

• GI: 151222582 NCBI
• RefSeq: YP_001333404 NCBI
• UniProt: A0A0H3KAF1 UniProt

⊟Protein sequence

• MTSKHQQKGSIVNRFLNSVEKIGNKLPDPSVLFFLMCVGLAIMTWVISLFNVSVKHPGTHQTIYIKNIISHDGFTMIMNDTIKNFSEFPALGLVLAVMIGIGVAEKTGYFDKLMISVVNRAPRFLILPTIILIGILGSTAGDAATIILPPLAAMLFIKIGYHPIAGLTMAYASAVGGFAANIVVGMQDALVYSFTEPATRIVSDSIKTNVAMNWYFIAASVVVLLPTILLVTTKLIIPRLGKYDDSLMHDDHEETSSHITDKEAHALKWANISFIVTIILLIITAIPEHSFLRNAKTGSLLDDAPLINGVGLIILVVFLVPGLVYGILSKEIKNTKDLGKMFGDAVGSMGTFIVIVFFAAQLLAYLKWSNLGIIAAVKGAKLLEHQNGIVLILGIIVLSAMVNMLIGSASAKWGILGPIFVPMLILIGFHPAFTQVIYRVGDSITNPITPMMPYLPLLLTYAQKYDKRMKLGALLSSLMPYSIALSIVWTVFVIIWFLLGIPVGPGGPIFVK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator: CodY (repression) regulon

  CodY

  (TF)

  important in Amino acid metabolism; RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications