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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_2460 [new locus tag: NWMN_RS14135 ]
  • pan locus tag?: SAUPAN006224000
  • symbol: NWMN_2460
  • pan gene symbol?: —
  • synonym:
  • product: aminotransferase, class I

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_2460 [new locus tag: NWMN_RS14135 ]
  • symbol: NWMN_2460
  • product: aminotransferase, class I
  • replicon: chromosome
  • strand: -
  • coordinates: 2706669..2707823
  • length: 1155
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGATCTCTAACAAATTAGCAAATATCCCTGATAGTTATTTCGGGAAAACGATGGGACGT
    AAAATCGAACATGGACCATTGCCATTAATTAATATGGCAGTTGGAATCCCTGATGGGCCC
    ACGCCACAAGGTATTATTGATCATTTTCAAAAAGCACTAACTATTCCTGAAAATCAAAAA
    TATGGTGCTTTTCATGGTAAAGAAGCGTTCAAGCAAGCTATTGTAGATTTTTATCAAAGA
    CAATATAATGTGACGTTAGATAAAGAAGATGAAGTATGCATTTTATATGGTACAAAAAAT
    GGATTAGTGGCAGTACCAACATGTGTTATCAATCCAGGAGACTATGTATTACTACCTGAT
    CCAGGCTACACGGATTATTTAGCAGGTGTACTTTTAGCTGATGGCAAGCCAGTCCCGCTT
    AATTTAGAACCGCCACATTATTTGCCAGATTGGTCCAAAGTTGATTCACAAATAATAGAT
    AAAACAAAACTCATTTATTTAACGTATCCAAATAATCCAACTGGATCGACAGCTACGAAA
    GAAGTTTTTGATGAAGCGATAGCTAAATTTAAAGGTACAGACACTAAAATCGTGCATGAT
    TTTGCTTATGGTGCTTTCGGTTTCGACGCTAAAAATCCTAGTATACTTGCTTCGGAAAAT
    GGGAAAGATGTTGCGATTGAAATTTATTCCTTGTCTAAAGGTTATAACATGTCAGGTTTT
    AGAGTCGGTTTTGCAGTTGGTAATAAAGATATGATTCAAGCTTTGAAAAAGTACCAAACG
    CACACAAATGCTGGGATGTTTGGTGCATTACAAGATGCCGCAATATATGCTTTGAATCAT
    TATGATGATTTTTTAGAAGAACAAAGCAATGTATTTAAAACACGTCGTGACCGATTCGAA
    GCCATGCTTGCAAAAGCTGATTTGCCATTTGTTCATGCTAAAGGTGGCATATATGTATGG
    TTGGAAACACCGCCTGGTTATGACAGCGAGCAATTTGAGCAATTTTTAGTGCAAGAGAAG
    TCAATACTTGTTGCTCCAGGGAAACCATTTGGAGAGAATGGTAATCGTTATGTGAGAATT
    TCATTGGCGTTAGATGACCAGAAATTAGACGAAGCTGCAATAAGATTAACAGAACTAGCA
    TATTTATATGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1155

⊟Protein[edit | edit source]

Protein Data Bank: 2O1B

⊟General[edit | edit source]

  • locus tag: NWMN_2460 [new locus tag: NWMN_RS14135 ]
  • symbol: NWMN_2460
  • description: aminotransferase, class I
  • length: 384
  • theoretical pI: 5.13624
  • theoretical MW: 42734.3
  • GRAVY: -0.280208

⊟Function[edit | edit source]

  • reaction:
    EC 2.6.1.-?  ExPASy
  • TIGRFAM:
    LL-diaminopimelate aminotransferase (TIGR03540; EC 2.6.1.83; HMM-score: 368.5)
    and 14 more
    succinyldiaminopimelate transaminase (TIGR03537; EC 2.6.1.17; HMM-score: 197.5)
    succinyldiaminopimelate transaminase (TIGR03539; EC 2.6.1.17; HMM-score: 167.1)
    succinyldiaminopimelate transaminase (TIGR03538; EC 2.6.1.17; HMM-score: 155.1)
    LL-diaminopimelate aminotransferase (TIGR03542; EC 2.6.1.83; HMM-score: 115.1)
    putative C-S lyase (TIGR04350; EC 4.4.-.-; HMM-score: 104)
    Metabolism Amino acid biosynthesis Histidine family histidinol-phosphate transaminase (TIGR01141; EC 2.6.1.9; HMM-score: 102)
    tyrosine/nicotianamine family aminotransferase (TIGR01265; HMM-score: 100.2)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin threonine-phosphate decarboxylase (TIGR01140; EC 4.1.1.81; HMM-score: 95.1)
    Cellular processes Cellular processes Biosynthesis of natural products capreomycidine synthase (TIGR03947; HMM-score: 92.7)
    Metabolism Energy metabolism Amino acids and amines tyrosine aminotransferase (TIGR01264; EC 2.6.1.5; HMM-score: 91.6)
    beta-methylarginine biosynthesis bifunctional aminotransferase (TIGR04544; EC 2.6.-.-; HMM-score: 89.4)
    enduracididine biosynthesis enzyme MppQ (TIGR04461; EC 2.-.-.-; HMM-score: 67.4)
    Metabolism Energy metabolism Amino acids and amines aspartate 4-decarboxylase (TIGR03801; EC 4.1.1.12; HMM-score: 23.5)
    enduracididine biosynthesis enzyme MppP (TIGR04462; EC 2.-.-.-; HMM-score: 13.7)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    PLP_aminotran (CL0061) Aminotran_1_2; Aminotransferase class I and II (PF00155; HMM-score: 207.8)
    and 2 more
    Alliinase_C; Allinase (PF04864; HMM-score: 24.9)
    DegT_DnrJ_EryC1; DegT/DnrJ/EryC1/StrS aminotransferase family (PF01041; HMM-score: 20.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: pyridoxal 5'-phosphate
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.010261
    • TAT(Tat/SPI): 0.000675
    • LIPO(Sec/SPII): 0.002956
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MISNKLANIPDSYFGKTMGRKIEHGPLPLINMAVGIPDGPTPQGIIDHFQKALTIPENQKYGAFHGKEAFKQAIVDFYQRQYNVTLDKEDEVCILYGTKNGLVAVPTCVINPGDYVLLPDPGYTDYLAGVLLADGKPVPLNLEPPHYLPDWSKVDSQIIDKTKLIYLTYPNNPTGSTATKEVFDEAIAKFKGTDTKIVHDFAYGAFGFDAKNPSILASENGKDVAIEIYSLSKGYNMSGFRVGFAVGNKDMIQALKKYQTHTNAGMFGALQDAAIYALNHYDDFLEEQSNVFKTRRDRFEAMLAKADLPFVHAKGGIYVWLETPPGYDSEQFEQFLVQEKSILVAPGKPFGENGNRYVRISLALDDQKLDEAAIRLTELAYLYE

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CodY (repression) regulon
    CodY(TF)important in Amino acid metabolism; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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