PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_2497 [new locus tag: NWMN_RS14330 ]
• pan locus tag^?: SAUPAN006294000
• symbol: panE
• pan gene symbol^?: panE
• synonym:
• product: 2-dehydropantoate 2-reductase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_2497 [new locus tag: NWMN_RS14330 ]
• symbol: panE
• product: 2-dehydropantoate 2-reductase
• replicon: chromosome
• strand: +
• coordinates: 2741902..2742762
• length: 861
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5332236 NCBI
• RefSeq: YP_001333531 NCBI
• BioCyc:
• MicrobesOnline: 3708099 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGTTATCAGTTGCGATTATCGGCCCAGGCGCTGTCGGTACAACTATTGCCTATGAATTA
  CAACAATCATTGCCTCATACAACGCTTATCGGGAGACACGCCAAAACAATAACATATTAT
  ACTGTACCACATGCACCTGCACAAGATATTGTTGTGAAAGGTTATGAAGATGTCACAAAC
  ACATTTGATGTTATTATTATTGCAGTCAAAACACATCAACTTGATGCAGTTATTCCTCAT
  TTAACTCATCTTGCACATGAGGACACGCTCATCATTTTAGCCCAAAATGGTTATGGTCAA
  CTCGAACATATTTCATTTAAAAACGTATGCCAAGCAGTTGTCTATATAAGTGGTCAAAAG
  AAAGGCGATGTTGTTACGCACTTCAGAGATTATCAATTACGTATACAAGATAATGCATTA
  ACTCGACAATTTAGAGATTTGGTCCAAGACAGTCAAATCGACATTGTATTAGAAGCAAAT
  ATTCAACAAGCTATTTGGTATAAATTGTTAGTTAACTTGGGCATTAATTCTATCACAGCA
  CTCGGTAGACAGACCGTTGCAATCATGCATAATCCTGAAATACGTACACTGTGTAGACAG
  CTATTACTAGATGGTTGTCGTGTTGCACAGGCAGAGGGGTTAAACTTTTCAGAACAAACT
  GTCGATACTATTATGACTATATATCAAGGTTATCCCGACGAAATGGGAACGAGCATGTAT
  TACGACATTGCGCATCAGCAACCTCTTGAAGTCGAAGCGATTCAAGGCTTTATTTATCGA
  CGTGCACGTGAACACAATCTTGACACCCCATATCTTGATACTATTTATAGCTTTTTACGC
  GCGTATCAACAAAATATGTAA
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⊟Protein

⊟General

• locus tag: NWMN_2497 [new locus tag: NWMN_RS14330 ]
• symbol: PanE
• description: 2-dehydropantoate 2-reductase
• length: 286
• theoretical pI: 6.06642
• theoretical MW: 32357.7
• GRAVY: -0.0632867

⊟Function

• reaction:
  EC 1.1.1.169?  ExPASy
  2-dehydropantoate 2-reductase (R)-pantoate + NADP⁺ = 2-dehydropantoate + NADPH
• TIGRFAM:
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pantothenate and coenzyme A 2-dehydropantoate 2-reductase (TIGR00745; EC 1.1.1.-; HMM-score: 176)
  and 1 more
  Metabolism Amino acid biosynthesis Glutamate family pyrroline-5-carboxylate reductase (TIGR00112; EC 1.5.1.2; HMM-score: 16.2)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  6PGD_C (CL0106) ApbA_C; Ketopantoate reductase PanE/ApbA C terminal (PF08546; HMM-score: 104.4)
  and 9 more
  NADP_Rossmann (CL0063) ApbA; Ketopantoate reductase PanE/ApbA (PF02558; HMM-score: 63.8)
  F420_oxidored; NADP oxidoreductase coenzyme F420-dependent (PF03807; HMM-score: 29.5)
  NAD_Gly3P_dh_N; NAD-dependent glycerol-3-phosphate dehydrogenase N-terminus (PF01210; HMM-score: 25.4)
  DAO; FAD dependent oxidoreductase (PF01266; HMM-score: 16.7)
  NAD_binding_2; NAD binding domain of 6-phosphogluconate dehydrogenase (PF03446; HMM-score: 16)
  Pyr_redox; Pyridine nucleotide-disulphide oxidoreductase (PF00070; HMM-score: 15.2)
  Rossmann-like; Rossmann-like domain (PF10727; HMM-score: 14.4)
  IlvN; Acetohydroxy acid isomeroreductase, NADPH-binding domain (PF07991; HMM-score: 14.3)
  Pyr_redox_2; Pyridine nucleotide-disulphide oxidoreductase (PF07992; HMM-score: 11.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 1.78
  • Cytoplasmic Membrane Score: 8.16
  • Cellwall Score: 0.06
  • Extracellular Score: 0.01
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.090277
  • TAT(Tat/SPI): 0.003487
  • LIPO(Sec/SPII): 0.016301
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151222709 NCBI
• RefSeq: YP_001333531 NCBI
• UniProt: A0A0H3KJ19 UniProt

⊟Protein sequence

• MLSVAIIGPGAVGTTIAYELQQSLPHTTLIGRHAKTITYYTVPHAPAQDIVVKGYEDVTNTFDVIIIAVKTHQLDAVIPHLTHLAHEDTLIILAQNGYGQLEHISFKNVCQAVVYISGQKKGDVVTHFRDYQLRIQDNALTRQFRDLVQDSQIDIVLEANIQQAIWYKLLVNLGINSITALGRQTVAIMHNPEIRTLCRQLLLDGCRVAQAEGLNFSEQTVDTIMTIYQGYPDEMGTSMYYDIAHQQPLEVEAIQGFIYRRAREHNLDTPYLDTIYSFLRAYQQNM

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications