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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_2517 [new locus tag: NWMN_RS14455 ]
  • pan locus tag?: SAUPAN006325000
  • symbol: cysG
  • pan gene symbol?: cysG
  • synonym:
  • product: precorrin-2 dehydrogenase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_2517 [new locus tag: NWMN_RS14455 ]
  • symbol: cysG
  • product: precorrin-2 dehydrogenase
  • replicon: chromosome
  • strand: -
  • coordinates: 2767458..2768063
  • length: 606
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGAATATGCCATTAATGATAGATTTAACAAATAAAAATGTCGTCATAGTTGGTGGAGGC
    GTCGTTGCAAGTCGTCGGGCACAAACATTAAATCAATACGTTGAACATATGACGGTCATC
    AGTCCGACAATCACTGAAAAACTTCAAAATATGGTAGATAACGGTGTCGTCATATGGAAA
    GAAAAAGAATTTGAACCAAGCGATATTGTAGACGCGTATCTAGTTATTGCAGCAACCAAT
    GAGCCACGTGTCAATGAAGCGGTAAAAAAAGCCTTACCTGAGCATGCCCTTTTTAATAAT
    GTTGGAGATGCATCAAATGGCAATGTTGTATTTCCAAGTGCACTACACCGCGACAAGCTA
    ACTATCAGTGTATCAACTGATGGTGCGAGTCCTAAGTTGACAAAATCAATTATGGCAGAG
    CTTGAGGCGTTATATCCACCATCATACAGTTCGTATATCGACTTTTTATATACTTGCCGA
    CAGAAAATAAAAGTACTTGATATAACATATAACGAAAAGCAACAGTTACTGTCACAAATT
    GTGTCACAAGAATATTTAAATCATGACAAACAAGCTCAATTTTTAGCGTGGTTGGATGTA
    AGATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    606

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_2517 [new locus tag: NWMN_RS14455 ]
  • symbol: CysG
  • description: precorrin-2 dehydrogenase
  • length: 201
  • theoretical pI: 5.66459
  • theoretical MW: 22541.7
  • GRAVY: -0.146766

⊟Function[edit | edit source]

  • reaction:
    EC 1.3.1.76  ExPASy
    Precorrin-2 dehydrogenase Precorrin-2 + NAD+ = sirohydrochlorin + NADH?
  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin siroheme synthase, N-terminal domain (TIGR01470; HMM-score: 113.1)
    and 1 more
    Metabolism Energy metabolism Electron transport thioredoxin-disulfide reductase (TIGR01292; EC 1.8.1.9; HMM-score: 14.4)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    NADP_Rossmann (CL0063) NAD_binding_7; Putative NAD(P)-binding (PF13241; HMM-score: 81.4)
    and 6 more
    no clan defined Sirohm_synth_M; Sirohaem biosynthesis protein central (PF14824; HMM-score: 26.6)
    NADP_Rossmann (CL0063) 2-Hacid_dh_C; D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain (PF02826; HMM-score: 19.9)
    Pyr_redox; Pyridine nucleotide-disulphide oxidoreductase (PF00070; HMM-score: 17.6)
    Pyr_redox_2; Pyridine nucleotide-disulphide oxidoreductase (PF07992; HMM-score: 16)
    Shikimate_DH; Shikimate / quinate 5-dehydrogenase (PF01488; HMM-score: 13.1)
    Actin_ATPase (CL0108) BcrAD_BadFG; BadF/BadG/BcrA/BcrD ATPase family (PF01869; HMM-score: 11.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.015806
    • TAT(Tat/SPI): 0.001753
    • LIPO(Sec/SPII): 0.005276
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNMPLMIDLTNKNVVIVGGGVVASRRAQTLNQYVEHMTVISPTITEKLQNMVDNGVVIWKEKEFEPSDIVDAYLVIAATNEPRVNEAVKKALPEHALFNNVGDASNGNVVFPSALHRDKLTISVSTDGASPKLTKSIMAELEALYPPSYSSYIDFLYTCRQKIKVLDITYNEKQQLLSQIVSQEYLNHDKQAQFLAWLDVR

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CymR* (repression) regulon
    CymR*(TF)important in Cysteine metabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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