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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_2528 [new locus tag: NWMN_RS14530 ]
  • pan locus tag?: SAUPAN006356000
  • symbol: estA
  • pan gene symbol?: estA
  • synonym:
  • product: tributyrin esterase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_2528 [new locus tag: NWMN_RS14530 ]
  • symbol: estA
  • product: tributyrin esterase
  • replicon: chromosome
  • strand: -
  • coordinates: 2779576..2780337
  • length: 762
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGGCTTATATTTCATTAAACTATCATTCACCAACAATTGGTATGCATCAAAATTTGACA
    GTCATTTTACCGGAAGATCAAAGCTTCTTTAATAGCGATACAACTGTTAAACCATTAAAA
    ACTTTAATGTTGTTACATGGATTATCAAGTGATGAAACGACATATATGAGATATACAAGC
    ATAGAAAGGTATGCGAATGAACACAAATTAGCTGTGATTATGCCCAATGTGGATCATAGC
    GCATATGCTAACATGGCATATGGTCATAGCTATTATGATTATATTTTGGAAGTGTATGAT
    TATGTTCATCAAATATTTCCACTTTCCAAAAAGCGTGATGACAATTTTATAGCAGGTCAC
    TCTATGGGAGGATATGGCACAATTAAATTTGCATTAACACAAGGGGATAAATTTGCCAAA
    GCTGTACCATTATCTGCTGTGTTTGAAGCGCAAAATTTAATGGATCTAGAGTGGAATGAT
    TTTTCAAAAGAGGCCATAATTGGCAATCTTTCAAGTGTTAAAGGAACTGAACATGATCCG
    TATTACTTGCTAGACAAAGCTGTAGCTGAAGATAAACAAATTCCAAAATTGCTCATTATG
    TGTGGTAAACAAGACTTTTTATATCAAGACAACTTAGATTTTATCGATTATTTATCACGC
    ATAAATGTTCCTTATCAATTTGAAGATGGACCAGGAGATCATGATTATGCATATTGGGAT
    CAAGCGATTAAGCGTGCTATAACATGGATGGTGAATGATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    762

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_2528 [new locus tag: NWMN_RS14530 ]
  • symbol: EstA
  • description: tributyrin esterase
  • length: 253
  • theoretical pI: 4.82893
  • theoretical MW: 29095.7
  • GRAVY: -0.334387

⊟Function[edit | edit source]

  • TIGRFAM:
    Cellular processes Cellular processes Detoxification S-formylglutathione hydrolase (TIGR02821; EC 3.1.2.12; HMM-score: 44.5)
    and 3 more
    Metabolism Fatty acid and phospholipid metabolism Degradation esterase, PHB depolymerase family (TIGR01840; EC 3.1.1.-; HMM-score: 19.3)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (TIGR03695; EC 4.2.99.20; HMM-score: 16.1)
    Metabolism Amino acid biosynthesis Aspartate family homoserine O-acetyltransferase (TIGR01392; EC 2.3.1.31; HMM-score: 13.8)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    AB_hydrolase (CL0028) Esterase; Putative esterase (PF00756; HMM-score: 55.8)
    and 7 more
    Hydrolase_4; Serine aminopeptidase, S33 (PF12146; HMM-score: 28.1)
    Peptidase_S9; Prolyl oligopeptidase family (PF00326; HMM-score: 26.9)
    Abhydrolase_3; alpha/beta hydrolase fold (PF07859; HMM-score: 16.3)
    Abhydrolase_6; Alpha/beta hydrolase family (PF12697; HMM-score: 14.1)
    Abhydrolase_5; Alpha/beta hydrolase family (PF12695; HMM-score: 13.8)
    Abhydrolase_1; alpha/beta hydrolase fold (PF00561; HMM-score: 13.6)
    DHFred (CL0387) DHFR_1; Dihydrofolate reductase (PF00186; HMM-score: 11.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002008
    • TAT(Tat/SPI): 0.000116
    • LIPO(Sec/SPII): 0.000385
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MAYISLNYHSPTIGMHQNLTVILPEDQSFFNSDTTVKPLKTLMLLHGLSSDETTYMRYTSIERYANEHKLAVIMPNVDHSAYANMAYGHSYYDYILEVYDYVHQIFPLSKKRDDNFIAGHSMGGYGTIKFALTQGDKFAKAVPLSAVFEAQNLMDLEWNDFSKEAIIGNLSSVKGTEHDPYYLLDKAVAEDKQIPKLLIMCGKQDFLYQDNLDFIDYLSRINVPYQFEDGPGDHDYAYWDQAIKRAITWMVND

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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