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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus Newman
- locus tag: NWMN_RS00575 [old locus tag: NWMN_0103 ]
- pan locus tag?: SAUPAN000986000
- symbol: NWMN_RS00575
- pan gene symbol?: capI
- synonym:
- product: capsular polysaccharide biosynthesis protein
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: NWMN_RS00575 [old locus tag: NWMN_0103 ]
- symbol: NWMN_RS00575
- product: capsular polysaccharide biosynthesis protein
- replicon: chromosome
- strand: +
- coordinates: 127351..128460
- length: 1110
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1081ATGAGAATTTTAAATATTGTATCGAGTAATATTGTTCAAGACCCAAGGGTACTTAAACAA
ATAGAAACAATTAAAGGCGTTACGGATGATTATAAAATTGTTGGAATGAATAATTCACAA
GCTACTAATAAGCGATTGGAAAATTTAGATTGTAATTATCGTTTGTTAGGTAGCAAGGTA
GATCCAAAAAATATTCTTTCTAAATTAATTAAGCGTATAAGATTTGCAACAGGTGTTATC
CGAGAAATTAAAGCTTATAAACCTGACGTGATTCATGCAAATGATTTCGACGTATTATTA
ATGGTCTATTTAAGCAATTATAAAAAAGCTAATATTGTTTATGATGCGCATGAAATATAT
GCGAAAAATGCCTTTATTAATAAAGTTCCACTTATTTCAAAGTTTGTAGAAAGTATAGAA
AAACACATAGTAAAACATCGTGTTAATGCCTTCGTAACAGTAAGTCATGCAGCAAAAGAA
TATTATCAATCTAAAGGATATAAGAAGGAAGCGAATGTTATTACGAATGCACCTATTTTA
AATGATAGCAGAGAATTTAAAGAAATCGAAAACTTTAAAGAAATTGTATATCAAGGTCAA
ATTGTAATGGACAGAGGATATGAAGAGTTTATTATTGCTTCATCAGCTTTTAAACAAAAT
GCTCCTTCATTCATAATTCGAGGGTTTGGTCCGCATGAAGAAGTGATAAAAGAACTGATT
AGTTATAACCCGGAAAATATTAGGTTGGATAAACCAGTTGAAGTAAAAGAATTGGTTGAT
AAGTTAGCAGAAAGTAATGTTGGTGTTGTCTTGACGAAACCAGTATCTATTAATTTTGAA
TATACAGTATCTAATAAAATTTTTGAATGTATACATGCTGGTTTACCAGTAATTTTATCT
CCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAA
GTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTT
AATCATTTACGTCAAAATGCAATTAAGGCGTCTAAAATTTTGAATTGGCAAATAGAGAGT
GAACGGTTAGTAGAATTATATAAATTTTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: NWMN_RS00575 [old locus tag: NWMN_0103 ]
- symbol: NWMN_RS00575
- description: capsular polysaccharide biosynthesis protein
- length: 369
- theoretical pI: 9.58625
- theoretical MW: 42580.1
- GRAVY: -0.242276
⊟Function[edit | edit source]
- TIGRFAM: PEP-CTERM/exosortase A-associated glycosyltransferase, Daro_2409 family (TIGR04063; EC 2.4.-.-; HMM-score: 14)
- TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
- PFAM: GT-B (CL0113) Glyco_transf_4; Glycosyltransferase Family 4 (PF13439; HMM-score: 40.4)and 5 moreGlyco_trans_1_4; Glycosyl transferases group 1 (PF13692; HMM-score: 25.3)Glycos_transf_1; Glycosyl transferases group 1 (PF00534; HMM-score: 19)Glyco_transf_5; Starch synthase catalytic domain (PF08323; HMM-score: 18.2)Glyco_trans_1_2; Glycosyl transferases group 1 (PF13524; HMM-score: 16.8)Glyco_trans_4_4; Glycosyl transferase 4-like domain (PF13579; HMM-score: 16.8)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.018724
- TAT(Tat/SPI): 0.000376
- LIPO(Sec/SPII): 0.002411
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MRILNIVSSNIVQDPRVLKQIETIKGVTDDYKIVGMNNSQATNKRLENLDCNYRLLGSKVDPKNILSKLIKRIRFATGVIREIKAYKPDVIHANDFDVLLMVYLSNYKKANIVYDAHEIYAKNAFINKVPLISKFVESIEKHIVKHRVNAFVTVSHAAKEYYQSKGYKKEANVITNAPILNDSREFKEIENFKEIVYQGQIVMDRGYEEFIIASSAFKQNAPSFIIRGFGPHEEVIKELISYNPENIRLDKPVEVKELVDKLAESNVGVVLTKPVSINFEYTVSNKIFECIHAGLPVILSPVKEHIYLNEKYKFGIVLKEVTPLEIEKAVRKLRDNHDLFNHLRQNAIKASKILNWQIESERLVELYKF
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator: CodY, SigB see NWMN_0103
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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