PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_RS00600 [old locus tag: NWMN_0108 ]
• pan locus tag^?: SAUPAN000992000
• symbol: NWMN_RS00600
• pan gene symbol^?: capN
• synonym:
• product: UDP-glucose 4-epimerase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_RS00600 [old locus tag: NWMN_0108 ]
• symbol: NWMN_RS00600
• product: UDP-glucose 4-epimerase
• replicon: chromosome
• strand: +
• coordinates: 132612..133499
• length: 888
• essential: unknown other strains

⊟Accession numbers

• Location: NC_009641 (132612..133499) NCBI
• BioCyc:
• MicrobesOnline: see NWMN_0108

⊟Phenotype

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⊟DNA sequence

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  ATGAGAAAAAATATTTTAATTACAGGCGTACATGGATATATCGGTAATGCTTTAAAAGAT
  AAGCTTATTGAACAAGGACATCAAGTAGATCAAATTAATGTTAGGAATCAATTATGGAAG
  TCGACCTCGTTCAAAGATTATGATGTTTTAATTCATACAGCAGCTTTGGTTCACAACAAT
  TCACCTCAAGCAAGGCTATCTGATTATATGCAAGTGAATATGTTGCTGACGAAACAATTG
  GCACAAAAGGCTAAAGCTGAAGACGTTAAACAATTTATTTTTATGAGTACTATGGCAGTT
  TATGGAAAAGAAGGTCATGTTGGTAAATCAGATCAAGTTGATACACAAACACCAATGAAC
  CCTACGACCAACTATGGTATTTCCAAAAAGTTCGCTGAACAAGCATTACAAGAATTGATT
  AGTGATTCGTTTAAAGTAGCAATTGTGAGACCACCAATGATTTATGGTGCACATTGCCCA
  GGAAATTTCCAACGGTTAATGCAATTGTCAAAGCGATTGCCAATCATTCCCAATATTAAC
  AATCAGCGCAGTGCATTATATATTAAACATCTGACAGCATTTATTGATCAATTAATATCA
  TTAGAAGTGACAGGTGTGTACCATCCTCAAGATAGTTTTTACTTTGATACATCGTCAGTA
  ATGTATGAAATACGTCGCCAATCACATCGTAAAACGGTATTGATCAACATGCCTTCAATG
  CTAAATAAGTATTTTAATAAGTTGTCGGTCTTTAGAAAATTATTCGGCAATTTAATATAC
  AGCAATACGTTATATGAAAATAATAATGCACTTGAAATTATTCCTGGAAAAATGTCACTT
  GTTATTGCGGACATCATGGATGAAACGACAACCAAAGATAAGGCATAA
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⊟Protein

⊟General

• locus tag: NWMN_RS00600 [old locus tag: NWMN_0108 ]
• symbol: NWMN_RS00600
• description: UDP-glucose 4-epimerase
• length: 295
• theoretical pI: 9.85632
• theoretical MW: 33682.7
• GRAVY: -0.292881

⊟Function

• TIGRFAM:
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides dTDP-4-dehydrorhamnose reductase (TIGR01214; EC 1.1.1.133; HMM-score: 66.4)
  Metabolism Energy metabolism Sugars UDP-glucose 4-epimerase GalE (TIGR01179; EC 5.1.3.2; HMM-score: 58.9)
  thioester reductase domain (TIGR01746; HMM-score: 54)
  and 9 more
  hopanoid-associated sugar epimerase (TIGR03466; HMM-score: 51)
  NAD dependent epimerase/dehydratase, LLPSF_EDH_00030 family (TIGR04180; HMM-score: 50.2)
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides ADP-glyceromanno-heptose 6-epimerase (TIGR02197; EC 5.1.3.20; HMM-score: 37.4)
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides dTDP-glucose 4,6-dehydratase (TIGR01181; EC 4.2.1.46; HMM-score: 31.9)
  L-aminoadipate-semialdehyde dehydrogenase (TIGR03443; EC 1.2.1.31; HMM-score: 27.6)
  Hypothetical proteins Conserved TIGR01777 family protein (TIGR01777; HMM-score: 23.3)
  UDP-N-acetylglucosamine 4,6-dehydratase (inverting) (TIGR03589; EC 4.2.1.115; HMM-score: 15.1)
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides GDP-mannose 4,6-dehydratase (TIGR01472; EC 4.2.1.47; HMM-score: 12.8)
  3-hydroxybutyrate dehydrogenase (TIGR01963; HMM-score: 12.1)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  NADP_Rossmann (CL0063) Epimerase; NAD dependent epimerase/dehydratase family (PF01370; HMM-score: 86.9)
  and 8 more
  NAD_binding_4; Male sterility protein (PF07993; HMM-score: 61.5)
  RmlD_sub_bind; RmlD substrate binding domain (PF04321; HMM-score: 55.7)
  3Beta_HSD; 3-beta hydroxysteroid dehydrogenase/isomerase family (PF01073; HMM-score: 51.1)
  GDP_Man_Dehyd; GDP-mannose 4,6 dehydratase (PF16363; HMM-score: 48.4)
  NAD_binding_10; NAD(P)H-binding (PF13460; HMM-score: 33.3)
  Polysacc_synt_2; Polysaccharide biosynthesis protein (PF02719; HMM-score: 33)
  no clan defined DnaB; DnaB-like helicase N terminal domain (PF00772; HMM-score: 15.5)
  NADP_Rossmann (CL0063) NmrA; NmrA-like family (PF05368; HMM-score: 12.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.01321
  • TAT(Tat/SPI): 0.000325
  • LIPO(Sec/SPII): 0.002067
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 447153414 NCBI
• RefSeq: WP_001230670 NCBI
• UniProt: see NWMN_0108

⊟Protein sequence

• MRKNILITGVHGYIGNALKDKLIEQGHQVDQINVRNQLWKSTSFKDYDVLIHTAALVHNNSPQARLSDYMQVNMLLTKQLAQKAKAEDVKQFIFMSTMAVYGKEGHVGKSDQVDTQTPMNPTTNYGISKKFAEQALQELISDSFKVAIVRPPMIYGAHCPGNFQRLMQLSKRLPIIPNINNQRSALYIKHLTAFIDQLISLEVTGVYHPQDSFYFDTSSVMYEIRRQSHRKTVLINMPSMLNKYFNKLSVFRKLFGNLIYSNTLYENNNALEIIPGKMSLVIADIMDETTTKDKA

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: CodY, SigB see NWMN_0108

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications