From AureoWiki
Jump to navigation Jump to search

NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS01100 [old locus tag: NWMN_0197 ]
  • pan locus tag?: SAUPAN001142000
  • symbol: NWMN_RS01100
  • pan gene symbol?: lrgB
  • synonym:
  • product: antiholin-like protein LrgB

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_RS01100 [old locus tag: NWMN_0197 ]
  • symbol: NWMN_RS01100
  • product: antiholin-like protein LrgB
  • replicon: chromosome
  • strand: +
  • coordinates: 254534..255235
  • length: 702
  • essential: unknown

Accession numbers[edit | edit source]

  • Location: NC_009641 (254534..255235) NCBI
  • BioCyc:
  • MicrobesOnline:

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGATTAACCACTTAGCACTAAACACACCTTACTTCGGAATACTGTTATCCGTTATACCA
    TTTTTCTTAGCGACCATATTATTTGAAAAAACTAATCGTTTCTTCTTATTCGCACCGCTA
    TTTGTCAGTATGGTATTTGGTGTGGCCTTCCTCTATTTAACAGGCATTCCGTATAAGACT
    TACAAAATAGGTGGAGACATTATTTACTTCTTCTTAGAACCGGCAACAATCTGTTTTGCG
    ATTCCGTTATATAAAAAGCGTGAAGTGCTTGTTAAACATTGGCATCGTATCATCGGAGGT
    ATTGGTATCGGTACAGTTGTAGCGTTATTAATTATTTTAACTTTTGCGAAGTTAGCACAA
    TTTGCCAATGATGTTATTTTATCAATGTTACCTCAAGCAGCAACTACAGCGATTGCGTTA
    CCAGTATCAGCTGGTATCGGTGGTATAAAAGAATTAACATCATTAGCAGTTATTTTAAAT
    GGTGTCATTATTTATGCCCTAGGTAATAAATTCTTAAAGCTTTTCCGAATTACTAACCCT
    ATTGCCCGAGGATTAGCACTTGGAACAAGTGGTCACACATTAGGTGTAGCACCAGCCAAA
    GAATTAGGACCTGTAGAAGAATCAATGGCAAGTATAGCTTTAGTGTTAGTTGGTGTAGTT
    GTTGTAGCAGTTGTGCCTGTCTTTGTAGCAATATTCTTCTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    702

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: NWMN_RS01100 [old locus tag: NWMN_0197 ]
  • symbol: NWMN_RS01100
  • description: antiholin-like protein LrgB
  • length: 233
  • theoretical pI: 10.0614
  • theoretical MW: 25097.2
  • GRAVY: 1.1176

Function[edit | edit source]

  • TIGRFAM:
    Hypothetical proteins Conserved TIGR00659 family protein (TIGR00659; HMM-score: 165.7)
  • TheSEED:
  • PFAM:
    no clan defined LrgB; LrgB-like family (PF04172; HMM-score: 221.4)

Structure, modifications & interactions[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 7
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001451
    • TAT(Tat/SPI): 0.000131
    • LIPO(Sec/SPII): 0.007351
  • predicted transmembrane helices (TMHMM): 7

Accession numbers[edit | edit source]

  • GI: 446529721 NCBI
  • RefSeq: WP_000607067 NCBI
  • UniProt:

Protein sequence[edit | edit source]

  • MINHLALNTPYFGILLSVIPFFLATILFEKTNRFFLFAPLFVSMVFGVAFLYLTGIPYKTYKIGGDIIYFFLEPATICFAIPLYKKREVLVKHWHRIIGGIGIGTVVALLIILTFAKLAQFANDVILSMLPQAATTAIALPVSAGIGGIKELTSLAVILNGVIIYALGNKFLKLFRITNPIARGLALGTSGHTLGVAPAKELGPVEESMASIALVLVGVVVVAVVPVFVAIFF

Experimental data[edit | edit source]

  • experimentally validated: no data available

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]