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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS01420 [old locus tag: NWMN_0257 ]
  • pan locus tag?: SAUPAN001239000
  • symbol: NWMN_RS01420
  • pan gene symbol?: nanA
  • synonym:
  • product: N-acetylneuraminate lyase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_RS01420 [old locus tag: NWMN_0257 ]
  • symbol: NWMN_RS01420
  • product: N-acetylneuraminate lyase
  • replicon: chromosome
  • strand: -
  • coordinates: 313178..314059
  • length: 882
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_009641 (313178..314059) NCBI
  • BioCyc:
  • MicrobesOnline: see NWMN_0257

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGAACAAAGATTTAAAAGGTTTATATGCAGCGTTACTCGTTCCTTTTGATGAAAATGGT
    CAAGTAAATGAACAAGGACTTAAACAAATTGCTCAAAATGCCATTGAAACTGAAGAATTA
    GACGGTCTTTATGTAAATGGTAGCTCAGGCGAAAACTTTTTATTAAATACAGAGCAGAAG
    AAGCAAGTTTTCAAGGTTGCCAAAGAGGCAGTTGGGGATAAAGTGAAATTGATTGCTCAA
    GTAGGTTCGTTAGATTTAAATGAAGCCATTGAACTTGGAAAATATGCCACAGAACTCGGT
    TATGATGCACTTTCTGCCGTAACACCATTCTACTATCCATTTACTTTTGAAGAAATTAGA
    GATTACTATTTCGATATTATCGAAGCAACTCAGAACAATATGATTATTTATGCAATACCA
    GATTTAACAGGTGTGAATATTTCTATTGAACAATTCAGCGAGCTATTTAATCATGAAAAA
    ATTGTTGGTGTTAAATATACAGCGCCAAACTTCTTCCTACTCGAACGTATTAGAAAAGCA
    TTCCCAGACAAATTAATCTTATCTGGCTTTGATGAAATGTTAGTTCAAGCTACGATTTCT
    GGCGTAGATGGTGCAATTGGTTCTACATATAACGTTAATGGTCGCCGTGCAAGAAAAATC
    TTCGACTTAGCACGACAAGGTCAAATTCAAGAAGCTTACCAACTGCAACATGATTCAAAC
    GACATTATCGAAACAGTGTTATCAATGGGGATTTATCCAACATTGAAAGAAATATTACGT
    CATCGTGGCATTGATGCAGGATTACCGAAAAGACCTTTCAAACCTTTTAACGAAGCACAT
    CGACAAACATTAGATCAACTCATTGCAAAATACGATTTATAG
    60
    120
    180
    240
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    360
    420
    480
    540
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    720
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    840
    882

⊟Protein[edit | edit source]

Protein Data Bank: 4AH7
Protein Data Bank: 4AHO
Protein Data Bank: 4AHP
Protein Data Bank: 4AHQ
Protein Data Bank: 4AMA
Protein Data Bank: 5A8G
Protein Data Bank: 5KZD
Protein Data Bank: 5KZE
Protein Data Bank: 5LKY

⊟General[edit | edit source]

  • locus tag: NWMN_RS01420 [old locus tag: NWMN_0257 ]
  • symbol: NWMN_RS01420
  • description: N-acetylneuraminate lyase
  • length: 293
  • theoretical pI: 4.70663
  • theoretical MW: 33042.3
  • GRAVY: -0.232423

⊟Function[edit | edit source]

  • reaction:
    EC 4.1.3.3?  ExPASy
    N-acetylneuraminate lyase N-acetylneuraminate = N-acetyl-D-mannosamine + pyruvate
  • TIGRFAM:
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides N-acetylneuraminate lyase (TIGR00683; EC 4.1.3.3; HMM-score: 367.8)
    Metabolism Central intermediary metabolism Amino sugars N-acetylneuraminate lyase (TIGR00683; EC 4.1.3.3; HMM-score: 367.8)
    and 3 more
    Metabolism Amino acid biosynthesis Aspartate family 4-hydroxy-tetrahydrodipicolinate synthase (TIGR00674; EC 4.3.3.7; HMM-score: 139.2)
    2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase (TIGR02313; EC 4.1.2.-; HMM-score: 103.3)
    5-dehydro-4-deoxyglucarate dehydratase (TIGR03249; EC 4.2.1.41; HMM-score: 56.9)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    TIM_barrel (CL0036) DHDPS; Dihydrodipicolinate synthetase family (PF00701; HMM-score: 282.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.016918
    • TAT(Tat/SPI): 0.000578
    • LIPO(Sec/SPII): 0.002025
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNKDLKGLYAALLVPFDENGQVNEQGLKQIAQNAIETEELDGLYVNGSSGENFLLNTEQKKQVFKVAKEAVGDKVKLIAQVGSLDLNEAIELGKYATELGYDALSAVTPFYYPFTFEEIRDYYFDIIEATQNNMIIYAIPDLTGVNISIEQFSELFNHEKIVGVKYTAPNFFLLERIRKAFPDKLILSGFDEMLVQATISGVDGAIGSTYNVNGRRARKIFDLARQGQIQEAYQLQHDSNDIIETVLSMGIYPTLKEILRHRGIDAGLPKRPFKPFNEAHRQTLDQLIAKYDL

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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