From AureoWiki
Jump to navigation Jump to search

NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS02000 [old locus tag: NWMN_0354 ]
  • pan locus tag?: SAUPAN001907000
  • symbol: NWMN_RS02000
  • pan gene symbol?: —
  • synonym:
  • product: mechanosensitive ion channel protein MscS

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_RS02000 [old locus tag: NWMN_0354 ]
  • symbol: NWMN_RS02000
  • product: mechanosensitive ion channel protein MscS
  • replicon: chromosome
  • strand: +
  • coordinates: 405624..406505
  • length: 882
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_009641 (405624..406505) NCBI
  • BioCyc:
  • MicrobesOnline: see NWMN_0354

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGAATCAAGTCATGAATATTATTTCATCTCTATTTGAGCCATTAACAAAAATAGAAACA
    TATGAAAACATTGCAACTAAAATCGCTATGATTGTTATTTATATTATCGTAGCCCTCATA
    GTTATTAAAATACTGAATAAAATGATTGAACAGGGATTTAAGATTCAAAATAAAAGTAAA
    AAGAGTAACAAAAAGCGCTCTAAAACTTTAATATCTCTTGTTCAAAATGTAGTGAAGTAT
    ATCGTTTGGTTTATAGTTATTACGACGATTTTAAGTAAATTTGGCATTAGTGTTGAAGGG
    GTAATTGCCAGTGCTGGTGTCGTAGGCTTAGCAGTAGGTTTTGGTGCTCAAACTATAGTT
    AAAGACGTAATTACAGGATTCTTTATTATATTTGAAAGCCAATTTGATGTAGGTGATTAT
    GTTAAGATAAATAACGGTGGTACAACTGTAGCAGAGGGAACAGTTAAATCAATTGGACTT
    CGTTCAACACGAATCAATACAATTTCAGGAGAATTAACAATTTTACCAAATAGTAGTATG
    GGTGAAATAACGAACTACTCAATTACAAATGGTACAGCTATCGTTAAAATTCCAGTGTCT
    GTCGAAGAAAACATTGATAATGTTGATAAAAAACTAAACAAACTATTTACTTCTTTACGT
    AGTAAATATTACTTATTTGTTAGTGATCCGGTTGTTATTGGTATTGATGCTATTGAAGAT
    ACAAGAGTAATATTGAGAATATCTGCAGAAACAATTCCAGGTGAAGGATTTGCTGGAGCT
    CGAATTATTCGCAAAGAAGTACAAAAAATGTTTTTACAAGAAGGTATTAAAACACCTCAA
    CCAATTATGACTGCTTATAATCATAGTGAAAACGGTGTTTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    882

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_RS02000 [old locus tag: NWMN_0354 ]
  • symbol: NWMN_RS02000
  • description: mechanosensitive ion channel protein MscS
  • length: 293
  • theoretical pI: 9.92456
  • theoretical MW: 32186.5
  • GRAVY: 0.303413

⊟Function[edit | edit source]

  • TIGRFAM:
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    no clan defined MS_channel; Mechanosensitive ion channel (PF00924; HMM-score: 134.6)
    and 3 more
    DUF751; Protein of unknown function (DUF751) (PF05421; HMM-score: 16.2)
    Peptidase_MA (CL0126) DUF3810; Protein of unknown function (DUF3810) (PF12725; HMM-score: 10.8)
    no clan defined Phage_holin_6_1; Bacteriophage holin of superfamily 6 (Holin_LLH) (PF09682; HMM-score: 8.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 3
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002443
    • TAT(Tat/SPI): 0.000144
    • LIPO(Sec/SPII): 0.000431
  • predicted transmembrane helices (TMHMM): 3

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNQVMNIISSLFEPLTKIETYENIATKIAMIVIYIIVALIVIKILNKMIEQGFKIQNKSKKSNKKRSKTLISLVQNVVKYIVWFIVITTILSKFGISVEGVIASAGVVGLAVGFGAQTIVKDVITGFFIIFESQFDVGDYVKINNGGTTVAEGTVKSIGLRSTRINTISGELTILPNSSMGEITNYSITNGTAIVKIPVSVEENIDNVDKKLNKLFTSLRSKYYLFVSDPVVIGIDAIEDTRVILRISAETIPGEGFAGARIIRKEVQKMFLQEGIKTPQPIMTAYNHSENGV

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=NWMN_RS02000&oldid=78731"