From AureoWiki
Jump to navigation Jump to search

NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS02555 [old locus tag: NWMN_0448 ]
  • pan locus tag?: SAUPAN002219000
  • symbol: NWMN_RS02555
  • pan gene symbol?: holB
  • synonym:
  • product: DNA polymerase III subunit delta'

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_RS02555 [old locus tag: NWMN_0448 ]
  • symbol: NWMN_RS02555
  • product: DNA polymerase III subunit delta'
  • replicon: chromosome
  • strand: +
  • coordinates: 511202..512128
  • length: 927
  • essential: unknown

Accession numbers[edit | edit source]

  • Location: NC_009641 (511202..512128) NCBI
  • BioCyc:
  • MicrobesOnline:

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGATGAACAGCAACAATTGACGAATGCATATCATTCAAATAAATTATCGCATGCCTAT
    TTATTTGAAGGTGATGATGCACAAACGATGAAACAAGTTGCGATTAATTTTGCAAAGCTT
    ATTTTATGTCAAACAGATAGTCAATGTGAAACAAAGGTTAGTACATATAATCATCCAGAC
    TTTATGTATATATCAACAACTGAGAATGCAATTAAGAAAGAACAAGTTGAACAACTTGTG
    CGTCATATGAATCAACTTCCTATAGAAAGCACAAATAAAGTGTACATCATTGAAGACTTT
    GAAAAGTTAACTGTTCAAGGGGAAAACAGTATCTTGAAATTTCTTGAAGAACCACCGGAC
    AATACGATTGCTATTTTATTGTCTACAAAACCTGAGCAAATTTTAGACACAATCCATTCA
    AGGTGTCAGCATGTATATTTCAAGCCTATTGATAAAGAAAAGTTTATAAATAGATTAGTT
    GAACAAAACATGTCTAAGCCAGTAGCTGAAATGATTAGTACTTATACTACGCAAATAGAT
    AATGCAATGGCTTTAAATGAAGAATTTGATTTATTAGCATTAAGGAAATCAGTTATACGT
    TGGTGTGAATTGTTGCTTACTAATAAGCCAATGGCACTTATAGGTATTATTGATTTATTG
    AAACAGGCTAAAAATAAAAAACTGCAATCTTTAACTATTGCAGCTGTGAATGGTTTCTTC
    GAAGATATCATACATACAAAGGTAAATGTAGAGGATAAACAAATATATAGTGATTTAAAA
    AATGATATTGATCAATATGCGCAAAAGTTGTCGTTTAATCAATTAATTTTGATGTTTGAT
    CAACTGACGGAAGCACATAAGAAATTGAATCAAAATGTAAATCCAACGCTTGTATTTGAA
    CAAATCGTAATTAAGGGTGTGAGTTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    927

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: NWMN_RS02555 [old locus tag: NWMN_0448 ]
  • symbol: NWMN_RS02555
  • description: DNA polymerase III subunit delta'
  • length: 308
  • theoretical pI: 5.41475
  • theoretical MW: 35515.6
  • GRAVY: -0.314286

Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA polymerase III, delta' subunit (TIGR00678; EC 2.7.7.7; HMM-score: 153.4)
    and 8 more
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA polymerase III, subunit gamma and tau (TIGR02397; EC 2.7.7.7; HMM-score: 102.8)
    Cellular processes Cellular processes Sporulation and germination ATP-dependent protease, Lon family (TIGR02903; EC 3.4.21.-; HMM-score: 18.7)
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides ATP-dependent protease, Lon family (TIGR02903; EC 3.4.21.-; HMM-score: 18.7)
    radical SAM/SPASM domain protein, ACGX system (TIGR04340; HMM-score: 14.9)
    mutatrotase, YjhT family (TIGR03547; EC 5.1.-.-; HMM-score: 13.6)
    Cellular processes Cellular processes Sporulation and germination ATP-dependent protease LonB (TIGR02902; EC 3.4.21.-; HMM-score: 13.1)
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides ATP-dependent protease LonB (TIGR02902; EC 3.4.21.-; HMM-score: 13.1)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone naphthoate synthase (TIGR01929; EC 4.1.3.36; HMM-score: 11.7)
  • TheSEED:
  • PFAM:
    P-loop_NTPase (CL0023) DNA_pol3_delta2; DNA polymerase III, delta subunit (PF13177; HMM-score: 155.9)
    and 2 more
    post-AAA (CL0604) DNApol3-delta_C; DNA polymerase III, delta subunit, C terminal (PF09115; HMM-score: 22.9)
    DNA_pol3_gamma3; DNA polymerase III subunits gamma and tau domain III (PF12169; HMM-score: 20.4)

Structure, modifications & interactions[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.032526
    • TAT(Tat/SPI): 0.004331
    • LIPO(Sec/SPII): 0.003335
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

  • GI: 446266482 NCBI
  • RefSeq: WP_000344337 NCBI
  • UniProt:

Protein sequence[edit | edit source]

  • MDEQQQLTNAYHSNKLSHAYLFEGDDAQTMKQVAINFAKLILCQTDSQCETKVSTYNHPDFMYISTTENAIKKEQVEQLVRHMNQLPIESTNKVYIIEDFEKLTVQGENSILKFLEEPPDNTIAILLSTKPEQILDTIHSRCQHVYFKPIDKEKFINRLVEQNMSKPVAEMISTYTTQIDNAMALNEEFDLLALRKSVIRWCELLLTNKPMALIGIIDLLKQAKNKKLQSLTIAAVNGFFEDIIHTKVNVEDKQIYSDLKNDIDQYAQKLSFNQLILMFDQLTEAHKKLNQNVNPTLVFEQIVIKGVS

Experimental data[edit | edit source]

  • experimentally validated: no data available

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]