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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS03015 [old locus tag: NWMN_0520 ]
  • pan locus tag?: SAUPAN002331000
  • symbol: NWMN_RS03015
  • pan gene symbol?: tadA
  • synonym:
  • product: tRNA-specific adenosine deaminase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_RS03015 [old locus tag: NWMN_0520 ]
  • symbol: NWMN_RS03015
  • product: tRNA-specific adenosine deaminase
  • replicon: chromosome
  • strand: +
  • coordinates: 599165..599635
  • length: 471
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_009641 (599165..599635) NCBI
  • BioCyc:
  • MicrobesOnline: see NWMN_0520

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    ATGACAAATGATATATATTTTATGACATTAGCGATTGAAGAAGCTAAAAAAGCAGCTCAA
    CTAGGCGAAGTACCTATAGGTGCTATCATCACTAAAGATGATGAAGTTATCGCTAGAGCA
    CATAATTTAAGAGAAACACTACAACAACCAACGGCGCATGCTGAACATATTGCAATTGAA
    CGTGCAGCCAAAGTGTTAGGTAGTTGGCGTTTAGAAGGTTGCACATTATATGTAACCTTA
    GAACCATGTGTCATGTGCGCAGGAACAATTGTAATGAGTCGCATTCCAAGAGTCGTCTAT
    GGCGCAGATGATCCTAAAGGTGGTTGTAGTGGCAGTTTAATGAATTTATTGCAACAATCT
    AATTTTAATCATCGTGCAATTGTTGATAAAGGTGTACTTAAAGAAGCATGTAGCACATTA
    TTAACAACATTTTTTAAAAACTTAAGAGCCAATAAGAAATCCACCAATTAG
    60
    120
    180
    240
    300
    360
    420
    471

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_RS03015 [old locus tag: NWMN_0520 ]
  • symbol: NWMN_RS03015
  • description: tRNA-specific adenosine deaminase
  • length: 156
  • theoretical pI: 8.21852
  • theoretical MW: 17089.7
  • GRAVY: -0.05

⊟Function[edit | edit source]

  • reaction:
    EC 3.5.4.33?  ExPASy
    tRNA(adenine34) deaminase Adenine34 in tRNA + H2O = hypoxanthine34 in tRNA + NH3
  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Riboflavin, FMN, and FAD riboflavin biosynthesis protein RibD (TIGR00326; EC 1.1.1.193,3.5.4.26; HMM-score: 53.9)
    and 1 more
    ComE operon protein 2 (TIGR02571; HMM-score: 29.3)
  • TheSEED: data available for N315, NCTC8325, USA300_FPR3757
  • PFAM:
    CDA (CL0109) MafB19-deam; MafB19-like deaminase (PF14437; HMM-score: 142.3)
    and 2 more
    dCMP_cyt_deam_1; Cytidine and deoxycytidylate deaminase zinc-binding region (PF00383; HMM-score: 100)
    Bd3614-deam; Bd3614-like deaminase (PF14439; HMM-score: 22.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Zn2+
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.006336
    • TAT(Tat/SPI): 0.000395
    • LIPO(Sec/SPII): 0.000756
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTNDIYFMTLAIEEAKKAAQLGEVPIGAIITKDDEVIARAHNLRETLQQPTAHAEHIAIERAAKVLGSWRLEGCTLYVTLEPCVMCAGTIVMSRIPRVVYGADDPKGGCSGSLMNLLQQSNFNHRAIVDKGVLKEACSTLLTTFFKNLRANKKSTN

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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