From AureoWiki
Jump to navigation Jump to search

NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS03450 [old locus tag: NWMN_0606 ]
  • pan locus tag?: SAUPAN002512000
  • symbol: NWMN_RS03450
  • pan gene symbol?: tagA
  • synonym:
  • product: N-acetylmannosaminyltransferase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_RS03450 [old locus tag: NWMN_0606 ]
  • symbol: NWMN_RS03450
  • product: N-acetylmannosaminyltransferase
  • replicon: chromosome
  • strand: +
  • coordinates: 686202..686966
  • length: 765
  • essential: unknown

Accession numbers[edit | edit source]

  • Location: NC_009641 (686202..686966) NCBI
  • BioCyc:
  • MicrobesOnline:

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGACTGTTGAAGAAAGATCCAATACAGCCAAAGTTGACATTTTAGGGGTCGATTTTGAT
    AATACAACAATGTTGCAAATGGTTGAAAATATTAAAACCTTTTTTGCAAATCAATCAACG
    AATAATCTTTTTATAGTAACAGCCAACCCTGAAATAGTGAATTACGCGACGACACATCAA
    GCGTATTTAGAGTTAATAAATCAAGCGAGCTATATTGTTGCTGATGGGACAGGAGTAGTC
    AAAGCTTCGCATCGTTTAAAGCAACCTCTAGCGCATCGTATACCTGGTATTGAGTTGATG
    GATGAATGTTTGAAAATTGCTCATGTAAATCATCAAAAAGTATTTTTGCTAGGGGCAACT
    AATGAAGTTGTAGAAGCGGCACAATATGCATTGCAACAAAGATATCCAAACATATCGTTT
    GCACATCATCACGGTTATATTGATTTAGAAGATGAGACAGTAGTGAAACGAATTAAACTG
    TTTAAACCTGATTACATATTTGTAGGTATGGGATTCCCTAAACAAGAAGAATGGATTATG
    ACACATGAAAACCAATTTGAATCTACAGTGATGATGGGCGTAGGTGGTTCTCTTGAAGTA
    TTTGCTGGGGCTAAAAAGAGAGCGCCTTATATCTTTAGAAAATTAAACATTGAATGGATA
    TATAGAGCATTAATAGATTGGAAACGTATTGGTAGATTAAAGAGTATTCCAATATTTATG
    TATAAAATAGCCAAAGCAAAAAGAAAAATAAAAAAGGCGAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    765

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: NWMN_RS03450 [old locus tag: NWMN_0606 ]
  • symbol: NWMN_RS03450
  • description: N-acetylmannosaminyltransferase
  • length: 254
  • theoretical pI: 9.77365
  • theoretical MW: 29132.7
  • GRAVY: -0.177953

Function[edit | edit source]

  • reaction:
    EC 2.4.1.187?  ExPASy
    N-acetylglucosaminyldiphosphoundecaprenol N-acetyl-beta-D-mannosaminyltransferase UDP-N-acetyl-alpha-D-mannosamine + N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol = UDP + N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
  • TIGRFAM:
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides glycosyltransferase, WecB/TagA/CpsF family (TIGR00696; EC 2.4.1.-; HMM-score: 160.5)
  • TheSEED:
  • PFAM:
    no clan defined Glyco_tran_WecB; Glycosyl transferase WecB/TagA/CpsF family (PF03808; HMM-score: 173.1)
    and 1 more
    P-loop_NTPase (CL0023) AAA_34; P-loop containing NTP hydrolase pore-1 (PF13872; HMM-score: 11.2)

Structure, modifications & interactions[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002866
    • TAT(Tat/SPI): 0.000398
    • LIPO(Sec/SPII): 0.000397
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

  • GI: 446137533 NCBI
  • RefSeq: WP_000215388 NCBI
  • UniProt:

Protein sequence[edit | edit source]

  • MTVEERSNTAKVDILGVDFDNTTMLQMVENIKTFFANQSTNNLFIVTANPEIVNYATTHQAYLELINQASYIVADGTGVVKASHRLKQPLAHRIPGIELMDECLKIAHVNHQKVFLLGATNEVVEAAQYALQQRYPNISFAHHHGYIDLEDETVVKRIKLFKPDYIFVGMGFPKQEEWIMTHENQFESTVMMGVGGSLEVFAGAKKRAPYIFRKLNIEWIYRALIDWKRIGRLKSIPIFMYKIAKAKRKIKKAK

Experimental data[edit | edit source]

  • experimentally validated: no data available

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]