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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS07010 [old locus tag: NWMN_1242 ]
  • pan locus tag?: SAUPAN003702000
  • symbol: NWMN_RS07010
  • pan gene symbol?: thrB
  • synonym:
  • product: homoserine kinase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_RS07010 [old locus tag: NWMN_1242 ]
  • symbol: NWMN_RS07010
  • product: homoserine kinase
  • replicon: chromosome
  • strand: +
  • coordinates: 1365724..1366638
  • length: 915
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_009641 (1365724..1366638) NCBI
  • BioCyc:
  • MicrobesOnline: see NWMN_1242

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGTCGAATGTTTTGGAGTTAACAATTCCTGCATCAACAGCCAACCTTGGAGTTGGCTTT
    GATTCTATAGGTATGGCTTTAGATAAATTTTTGCATCTGTCTGTAAAGGAAACATCAGGG
    ACAAAATGGGAATATATTTTCCATGATGATGCATCTAAGCAATTGCCTACTGACGAAACA
    AACTTTATTTATCATGTAGCACAACAAGTTGCTTCTAAATATAGTGTTGACTTGCCTAAT
    TTATGTATCGAAATGAGAAGTGATATTCCATTGGCAAGAGGGTTAGGTTCGTCAGCTTCT
    GCTTTAGTAGGAGCTATATATATCGCAAATTATTTTGGTGATATCCAACTGTCTAAACAT
    GAGGTATTACAATTAGCGACTGAAATCGAAGGACATCCTGATAATGTTGCGCCGACCATT
    TATGGTGGTTTAATCGCTGGATATTATAATGATGTCTCGAAAGAAACGTCAGTTGCACAT
    ATCGACATACCAGACGTGGATGTGATTGTAACGATACCAACTTATGAACTAAAAACAGAA
    GCATCAAGACGTGCTTTACCACAAAAATTAACACATAGTGAAGCGGTTAAAAGTAGTGCA
    ATTAGTAATACAATGATTTGTGCATTAGCACAGCACAATTATGAATTAGCAGGTAAACTC
    ATGCAACAAGATGGCTTTCATGAACCGTATCGTCAGCATTTAATTGCTGAATTTGATGAA
    GTGAAAACAATTGCTAGTCAACATAATGCCTATGCAACTGTAATTAGTGGTGCTGGACCA
    ACTATTTTAATATTTAGTCGTAAAGAAAATAGTGGGGAATTGGTTCGCTCTTTAAATAGT
    CAGGTAGTATCATGCCATTCTGAATTGGTCGATATTAATATCAGTGGTGTTAAAGAACGA
    ATTGTATACCAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    915

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_RS07010 [old locus tag: NWMN_1242 ]
  • symbol: NWMN_RS07010
  • description: homoserine kinase
  • length: 304
  • theoretical pI: 5.15013
  • theoretical MW: 33266.4
  • GRAVY: -0.0641447

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.1.39?  ExPASy
    Homoserine kinase ATP + L-homoserine = ADP + O-phospho-L-homoserine
  • TIGRFAM:
    Metabolism Amino acid biosynthesis Aspartate family homoserine kinase (TIGR00191; EC 2.7.1.39; HMM-score: 260.2)
    and 6 more
    Metabolism Central intermediary metabolism Other mevalonate kinase (TIGR00549; EC 2.7.1.36; HMM-score: 47)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase (TIGR00154; EC 2.7.1.148; HMM-score: 29.9)
    Metabolism Amino acid biosynthesis Aromatic amino acid family shikimate kinase (TIGR01920; EC 2.7.1.71; HMM-score: 28.8)
    Metabolism Central intermediary metabolism Other phosphomevalonate kinase (TIGR01220; EC 2.7.4.2; HMM-score: 22.9)
    Metabolism Central intermediary metabolism Other diphosphomevalonate decarboxylase (TIGR01240; EC 4.1.1.33; HMM-score: 16.9)
    Metabolism Energy metabolism Sugars galactokinase (TIGR00131; EC 2.7.1.6; HMM-score: 12.5)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    S5 (CL0329) GHMP_kinases_N; GHMP kinases N terminal domain (PF00288; HMM-score: 52.9)
    and 2 more
    no clan defined GHMP_kinases_C; GHMP kinases C terminal (PF08544; HMM-score: 34.9)
    DUF3963; Protein of unknown function (DUF3963) (PF13124; HMM-score: 12.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 3.33
    • Cellwall Score: 3.33
    • Extracellular Score: 3.33
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.013793
    • TAT(Tat/SPI): 0.00101
    • LIPO(Sec/SPII): 0.001581
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSNVLELTIPASTANLGVGFDSIGMALDKFLHLSVKETSGTKWEYIFHDDASKQLPTDETNFIYHVAQQVASKYSVDLPNLCIEMRSDIPLARGLGSSASALVGAIYIANYFGDIQLSKHEVLQLATEIEGHPDNVAPTIYGGLIAGYYNDVSKETSVAHIDIPDVDVIVTIPTYELKTEASRRALPQKLTHSEAVKSSAISNTMICALAQHNYELAGKLMQQDGFHEPYRQHLIAEFDEVKTIASQHNAYATVISGAGPTILIFSRKENSGELVRSLNSQVVSCHSELVDINISGVKERIVYQ

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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