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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS07225 [old locus tag: NWMN_1282 ]
  • pan locus tag?: SAUPAN003765000
  • symbol: NWMN_RS07225
  • pan gene symbol?: trpC
  • synonym:
  • product: indole-3-glycerol-phosphate synthase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_RS07225 [old locus tag: NWMN_1282 ]
  • symbol: NWMN_RS07225
  • product: indole-3-glycerol-phosphate synthase
  • replicon: chromosome
  • strand: +
  • coordinates: 1413367..1414149
  • length: 783
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_009641 (1413367..1414149) NCBI
  • BioCyc:
  • MicrobesOnline: see NWMN_1282

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGACGATTTTATCAGAAATTGTTAAATATAAACAGTCACTTTTACAAAATGGCTATTAT
    CAAGACAAACTTAATACCTTGAAAAGTGTGAAGATTCAGAATAAAAAATCTTTTATAAAC
    GCAATTGAGAAAGAACCAAAGCTAGCAATTATTGCAGAAATTAAATCGAAGAGTCCTACA
    GTTAATGACTTACCTGAACGAGATTTATCGCAACAAATCTCAGATTATGACCAATATGGT
    GCAAATGCCGTGTCCATTTTAACTGATGAAAAGTACTTTAGTGGTAGTTTTGAAAGATTA
    CAAGCATTGACGACAAAAACAACATTACCCGTATTATGCAAAGACTTTATTATAGACCCG
    CTTCAAATTGATGTTGCTAAACAAGCTGGTGCATCTATGATTTTATTGATCGTTAACATC
    TTATCTGATAAACAATTGAAAGATTTATATAACTACGCTATATCGCAAAATCTAGAAGTG
    TTAGTTGAAGTACATGATCGCCATGAATTAGAACGTGCCTATAAGGTTAATGCTAAATTG
    ATTGGTGTAAATAACAGGGACTTAAAACGATTTGTTACAAATGTGGAACATACAAATACT
    ATTTTAGAAAATAAAAAAACAAATCATTATTATATTTCTGAAAGTGGTATTCACGATGCA
    TCTGATGTAAGAAAAATCTTGCATAGTGGTATCGATGGCTTACTAATAGGTGAGGCGCTT
    ATGCGTTGTGACAATCTATCTGAATTTTTACCACAACTGAAAATGCAAAAGGTGAAGTCA
    TGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    783

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_RS07225 [old locus tag: NWMN_1282 ]
  • symbol: NWMN_RS07225
  • description: indole-3-glycerol-phosphate synthase
  • length: 260
  • theoretical pI: 8.18401
  • theoretical MW: 29556.8
  • GRAVY: -0.311923

⊟Function[edit | edit source]

  • reaction:
    EC 4.1.1.48?  ExPASy
    Indole-3-glycerol-phosphate synthase 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate = 1-C-(3-indolyl)-glycerol 3-phosphate + CO2 + H2O
  • TIGRFAM:
    Metabolism Amino acid biosynthesis Histidine family 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase (TIGR00007; EC 5.3.1.16; HMM-score: 14.8)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pyridine nucleotides nicotinate-nucleotide diphosphorylase (carboxylating) (TIGR00078; EC 2.4.2.19; HMM-score: 14)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    TIM_barrel (CL0036) IGPS; Indole-3-glycerol phosphate synthase (PF00218; HMM-score: 253)
    and 4 more
    QRPTase_C; Quinolinate phosphoribosyl transferase, C-terminal domain (PF01729; HMM-score: 22)
    FMN_dh; FMN-dependent dehydrogenase (PF01070; HMM-score: 15)
    FAD-oxidase_C (CL0277) BBE; Berberine and berberine like (PF08031; HMM-score: 12.5)
    TIM_barrel (CL0036) His_biosynth; Histidine biosynthesis protein (PF00977; HMM-score: 12.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003906
    • TAT(Tat/SPI): 0.000132
    • LIPO(Sec/SPII): 0.000493
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTILSEIVKYKQSLLQNGYYQDKLNTLKSVKIQNKKSFINAIEKEPKLAIIAEIKSKSPTVNDLPERDLSQQISDYDQYGANAVSILTDEKYFSGSFERLQALTTKTTLPVLCKDFIIDPLQIDVAKQAGASMILLIVNILSDKQLKDLYNYAISQNLEVLVEVHDRHELERAYKVNAKLIGVNNRDLKRFVTNVEHTNTILENKKTNHYYISESGIHDASDVRKILHSGIDGLLIGEALMRCDNLSEFLPQLKMQKVKS

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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