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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus Newman
- locus tag: NWMN_RS07440 [old locus tag: NWMN_1322 ]
- pan locus tag?: SAUPAN003827000
- symbol: NWMN_RS07440
- pan gene symbol?: —
- synonym:
- product: hypothetical protein
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: NWMN_RS07440 [old locus tag: NWMN_1322 ]
- symbol: NWMN_RS07440
- product: hypothetical protein
- replicon: chromosome
- strand: -
- coordinates: 1453280..1454071
- length: 792
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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781ATGGCACTAAAACATTATAAGAATTCAGATTCAACAGTTTTCAATGATGCGAAGGCATTA
TTTGATTTAAATAAAAATATTTTACTTAAAGGTCCAACAGGTTCAGGGAAAACAAAGTTG
GCAGAAACATTAAGTGAAGTTGTTGATACACCCATGCATCAAGTCAATTGTTCTGTTGAT
TTAGATACAGAAAGCTTATTAGGCTTTAAAACAATTAAAACAAATGCGGAAGGTCAACAA
GAAATTGTCTTTGTAGATGGTCCAGTTATTAAAGCTATGAAAGAGGGGCATATTTTATAT
ATTGATGAAATAAATATGGCTAAACCTGAAACATTGCCTGTATTAAATGGGGTCTTAGAT
TATCGTCGTCAAATTACGAATCCATACACTGGTGAAGTAATCAAAGCTGTACCAGGATTT
AACGTTATAGCAGCGATAAATGAAGGTTATGTTGGTACTTTGCCAATGAATGAAGCACTA
AAAAATCGCTTTGTTGTTATTCACGTTGATTATATTGATGGGGACATTTTAAAAAATGTG
ATTAAAGAGCAAAGTTTATTACAAGATGATAAACAAATCGAACAAATTATTAAGTTTAAT
GAAGATTTACGTACTATGTCTAAGCAGGGACAAATTTCTGAAGAAGCCGCTAGTATCCGT
GCATTATTAGACTTGTGTGATTTAATCACTGTAATGCCAGTTGAACGTGCAATTAAACGT
ACAATTATTGATAAATTGGAAGATGAACGTGAACAACAAGCAATATATAATGCTGTAGAA
CTAAACTTTTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: NWMN_RS07440 [old locus tag: NWMN_1322 ]
- symbol: NWMN_RS07440
- description: hypothetical protein
- length: 263
- theoretical pI: 4.68695
- theoretical MW: 29448.6
- GRAVY: -0.186312
⊟Function[edit | edit source]
- TIGRFAM: Cellular processes Other gas vesicle protein GvpN (TIGR02640; HMM-score: 75.1)and 11 moreBiosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin cobaltochelatase, CobS subunit (TIGR01650; EC 6.6.1.2; HMM-score: 34.8)Protein fate Degradation of proteins, peptides, and glycopeptides ATP-dependent Clp protease, ATP-binding subunit ClpX (TIGR00382; HMM-score: 30.2)Protein fate Protein folding and stabilization ATP-dependent Clp protease, ATP-binding subunit ClpX (TIGR00382; HMM-score: 30.2)DNA metabolism DNA replication, recombination, and repair Holliday junction DNA helicase RuvB (TIGR00635; EC 3.6.4.12; HMM-score: 27.8)Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll magnesium chelatase ATPase subunit D (TIGR02031; EC 6.6.1.1; HMM-score: 20.5)Protein fate Protein folding and stabilization ATP-dependent protease HslVU, ATPase subunit (TIGR00390; HMM-score: 18.7)Protein synthesis tRNA and rRNA base modification tRNA 2-selenouridine synthase (TIGR03167; EC 2.9.1.-; HMM-score: 14.3)DNA metabolism DNA replication, recombination, and repair orc1/cdc6 family replication initiation protein (TIGR02928; HMM-score: 14.1)Cellular processes Conjugation P-type conjugative transfer ATPase TrbB (TIGR02782; HMM-score: 13.6)thiol reductant ABC exporter, CydC subunit (TIGR02868; HMM-score: 13.3)Unknown function General Mg chelatase-like protein (TIGR00368; HMM-score: 12.2)
- TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
- PFAM: P-loop_NTPase (CL0023) AAA_5; AAA domain (dynein-related subfamily) (PF07728; HMM-score: 98.8)and 25 moreAAA_3; ATPase family associated with various cellular activities (AAA) (PF07726; HMM-score: 45.1)Sigma54_activat; Sigma-54 interaction domain (PF00158; HMM-score: 25)RuvB_N; Holliday junction DNA helicase ruvB N-terminus (PF05496; HMM-score: 23.1)no clan defined CbbQ_C; CbbQ/NirQ/NorQ C-terminal (PF08406; HMM-score: 23.1)P-loop_NTPase (CL0023) AAA; ATPase family associated with various cellular activities (AAA) (PF00004; HMM-score: 22.9)AAA_2; AAA domain (Cdc48 subfamily) (PF07724; HMM-score: 21.4)AAA_14; AAA domain (PF13173; HMM-score: 20.9)Mg_chelatase; Magnesium chelatase, subunit ChlI (PF01078; HMM-score: 19.4)T2SSE; Type II/IV secretion system protein (PF00437; HMM-score: 19.1)AAA_18; AAA domain (PF13238; HMM-score: 17.6)AAA_16; AAA ATPase domain (PF13191; HMM-score: 16.5)IstB_IS21; IstB-like ATP binding protein (PF01695; HMM-score: 16.4)ATP_bind_1; Conserved hypothetical ATP binding protein (PF03029; HMM-score: 15.7)ABC_tran; ABC transporter (PF00005; HMM-score: 15.6)AAA_6; Hydrolytic ATP binding site of dynein motor region D1 (PF12774; HMM-score: 15.3)AAA_22; AAA domain (PF13401; HMM-score: 14.5)AAA_29; P-loop containing region of AAA domain (PF13555; HMM-score: 14.3)CPT; Chloramphenicol phosphotransferase-like protein (PF07931; HMM-score: 14.2)TsaE; Threonylcarbamoyl adenosine biosynthesis protein TsaE (PF02367; HMM-score: 14.1)AAA_PrkA; PrkA AAA domain (PF08298; HMM-score: 13.8)ResIII; Type III restriction enzyme, res subunit (PF04851; HMM-score: 13.7)AAA_19; AAA domain (PF13245; HMM-score: 12.5)NTPase_1; NTPase (PF03266; HMM-score: 12.4)AAA_30; AAA domain (PF13604; HMM-score: 12.1)AAA_24; AAA domain (PF13479; HMM-score: 12)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.006207
- TAT(Tat/SPI): 0.000694
- LIPO(Sec/SPII): 0.000454
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MALKHYKNSDSTVFNDAKALFDLNKNILLKGPTGSGKTKLAETLSEVVDTPMHQVNCSVDLDTESLLGFKTIKTNAEGQQEIVFVDGPVIKAMKEGHILYIDEINMAKPETLPVLNGVLDYRRQITNPYTGEVIKAVPGFNVIAAINEGYVGTLPMNEALKNRFVVIHVDYIDGDILKNVIKEQSLLQDDKQIEQIIKFNEDLRTMSKQGQISEEAASIRALLDLCDLITVMPVERAIKRTIIDKLEDEREQQAIYNAVELNF
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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