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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus Newman
- locus tag: NWMN_RS07705 [old locus tag: NWMN_1368 ]
- pan locus tag?: SAUPAN003917000
- symbol: NWMN_RS07705
- pan gene symbol?: papS
- synonym:
- product: CCA-adding enzyme
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: NWMN_RS07705 [old locus tag: NWMN_1368 ]
- symbol: NWMN_RS07705
- product: CCA-adding enzyme
- replicon: chromosome
- strand: -
- coordinates: 1534444..1535646
- length: 1203
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
- 1
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1201ATGGATAAATCATTATTTGAACAGGCAAGGCCTATATTAGAACAAATTCAAGACAATGGT
TTTGAAGCATATTATGTAGGTGGCTCTGTAAGAGATTATGTCATGGGAAGAAATATTCAT
GATATAGATATCACAACAAGTGCAACGCCGGATGAAATAGAATCTATCTTTAGTCATACG
ATACCTGTAGGTAAAGAACATGGCACGATAAATGTAGTTTTTAATGATGAAAATTATGAA
GTGACAACATTCCGGGCTGAAGAAGATTATGTCGATCACCGTAGACCAAGTGGTGTTACA
TTTGTTCGTGATTTATACGAAGATTTGCAACGACGAGATTTCACGATGAATGCGATAGCA
ATGGATACAGCATACAAATTGTATGATTATTTTGATGGTCAACAAGATATTAATAATCGA
ATAATAAGAACTGTAGGTATAGCTGAGGAACGATTCCAAGAAGATGCTTTACGTATGATT
CGATGTTTAAGGTTCCAGTCACAATTATCATTTGATATTGCAACGGAAACATTCGAAGCG
ATGCGTATACAAATGGCAGATATTAAATTTTTATCAATTGAGCGTATAGTGATTGAACTA
ACTAAATTAATGCGAGGTATTAATGTTGAAAAGAGTTTTAATCATTTAAAATCGCTGAAA
GCATTTAATTATATGCCGTATTTCGAACATCTTGATATGAATCAAATTAATGTAACTGAA
GCAATTGATTTAGAATTGTTGATTGCTATAGTATCAGTTAAATTTGATATTAATTACTCA
TTGAAGCCTTTAAAGCTAAGTAACCGACAAGTAAAAGATATCAATCAATATATTCAAATT
ATGAATGCATTACCAAGTATTATTACAAAAGAACAATTAAAAATGTTTGTTTATGATTAT
GATACGCATCTCATTAAAAATGTAATGGTTGCAGCAGACGTGTTAAAGGCAAATGATATT
CAAGGACATGAACCATTAATCGTTAACCTTCAAACGATTGATGAAACATTACATCGTTTA
CCTATGCATAATAGAAAAGACATGATGGTTAATGGCGGTGTACTTATGGCACATTTAAAT
GCCAAAAGTGGTCCGTGGTTAAAAGATGTGCTAAGACAAATTGAGATAGCGATTGTAACA
GGTAAAGTAAGCAACGAAGAAACTGAAATTTTGAAATGGGTGGATAATCATGTCAAAATA
TAG60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: NWMN_RS07705 [old locus tag: NWMN_1368 ]
- symbol: NWMN_RS07705
- description: CCA-adding enzyme
- length: 400
- theoretical pI: 5.1044
- theoretical MW: 46435.9
- GRAVY: -0.247
⊟Function[edit | edit source]
- reaction: EC 2.7.7.72? ExPASyCCA tRNA nucleotidyltransferase A tRNA precursor + 2 CTP + ATP = a tRNA with a 3' CCA end + 3 diphosphate
- TIGRFAM: poly(A) polymerase (TIGR01942; EC 2.7.7.19; HMM-score: 161.7)Protein synthesis tRNA and rRNA base modification CCA tRNA nucleotidyltransferase (TIGR02692; EC 2.7.7.72; HMM-score: 161.1)
- TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
- PFAM: NTP_transf (CL0260) PolyA_pol; Poly A polymerase head domain (PF01743; HMM-score: 125.7)and 2 moreHD_PDEase (CL0237) tRNA_NucTran2_2; tRNA nucleotidyltransferase domain 2 putative (PF13735; HMM-score: 83.8)no clan defined PolyA_pol_RNAbd; Probable RNA and SrmB- binding site of polymerase A (PF12627; HMM-score: 34.9)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors: Mg2+
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.004999
- TAT(Tat/SPI): 0.000857
- LIPO(Sec/SPII): 0.000527
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MDKSLFEQARPILEQIQDNGFEAYYVGGSVRDYVMGRNIHDIDITTSATPDEIESIFSHTIPVGKEHGTINVVFNDENYEVTTFRAEEDYVDHRRPSGVTFVRDLYEDLQRRDFTMNAIAMDTAYKLYDYFDGQQDINNRIIRTVGIAEERFQEDALRMIRCLRFQSQLSFDIATETFEAMRIQMADIKFLSIERIVIELTKLMRGINVEKSFNHLKSLKAFNYMPYFEHLDMNQINVTEAIDLELLIAIVSVKFDINYSLKPLKLSNRQVKDINQYIQIMNALPSIITKEQLKMFVYDYDTHLIKNVMVAADVLKANDIQGHEPLIVNLQTIDETLHRLPMHNRKDMMVNGGVLMAHLNAKSGPWLKDVLRQIEIAIVTGKVSNEETEILKWVDNHVKI
⊟Experimental data[edit | edit source]
- experimentally validated: data available for COL, NCTC8325
- protein localization: data available for COL
- quantitative data / protein copy number per cell:
- interaction partners:
NWMN_RS12340 30S ribosomal protein S5 [1] (data from MRSA252)
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
J Proteome Res: 2011, 10(3);1139-50
[PubMed:21166474] [WorldCat.org] [DOI] (I p)