PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_RS08195 [old locus tag: NWMN_1452 ]
• pan locus tag^?: SAUPAN004129000
• symbol: NWMN_RS08195
• pan gene symbol^?: —
• synonym:
• product: rhomboid family intramembrane serine protease

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_RS08195 [old locus tag: NWMN_1452 ]
• symbol: NWMN_RS08195
• product: rhomboid family intramembrane serine protease
• replicon: chromosome
• strand: -
• coordinates: 1619257..1620720
• length: 1464
• essential: unknown other strains

⊟Accession numbers

• Location: NC_009641 (1619257..1620720) NCBI
• BioCyc:
• MicrobesOnline: see NWMN_1452

⊟Phenotype

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⊟DNA sequence

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  ATGAACATAGACAAACAATTTTGGAAAACAATATATTATTGGATTAGGTACTTGAATTTT
  GATATTGTCAGTAGGGAAAAAGATGACCAAGAAATTTGGTTAGCCCATAAAAGAAAAAAA
  CAAGTAGTCATTTTTAAACAACATATCAAGTCTACCCAAGAAATACGTTTTGACAAAGCG
  AAAGTGCTTGAACACAAAGATGAAATAGCAAATTTTATTTCTTTCGAACCACAAAGTTTT
  GAATTTTATTATTTTACAGAATCGGAATTTTCAGAAGAACAATTAAATGAAGTTTCGCCA
  ATTAGAATTAAATTCAATGTTATAAGACACACAAAAGATTTGATAAAGCATATGCCGAAT
  ATATTTTTGGCTAGACTTATTTCAGAAGATAATGATAAAAAGACATATATGTTCTATAAA
  CGCAAAGTATTAACCGATAACTTTTTAGATAAATATATGCAGAAATTTTCACCGGCAACA
  TACACAATAATATTTGTAAATGTCTTAATATGGTTATGTATGATTTTATATTTAAATAAT
  TTTTCGGATGTAAAATTATTAGATGTTGGCGGGTTAGTGCATTTTAATGTCGTACATGGT
  GAATGGTATCGAATTGTTACATCGATGTTTTTACATTTTAGTTTTGAACATATACTTATG
  AATATGCTTTCATTATTTATTTTTGGTAAAATAGTCGAAGCAATTATTGGTTCATGGCGG
  ATGTTAACTGTATACTTTATTGCAGGGTTGTTTGGAAACTTTGTATCACTATCATTTAAT
  ACGACTACAATTTCAGTTGGGGCTAGTGGTGCTATATTTGGTCTGATTGGATCAATTTTT
  GCGATGATGTATGTTTCAAAAACATTTAACAAAAAAATGTTAGGACAGTTATTAATTGCA
  TTAGTGATATTAGTTGGTGTTTCTCTGTTTATGTCAAATATAAATATTGTGGCGCATATT
  GGAGGATTCATTGGTGGTTTATTAATAACTTTAATTGGCTATTACTATAAAGTGAATCGT
  AATATTTTTTGGATTTTACTAATTGGTATGCTTGTTATATTTATTGCACTTCAAATTAGA
  ATTTTTACAATTAAAGAAGATAATATTTATAATAAATTGATCAAAGATGATATGACTAGT
  GGTAATTATGATAATGCTCAAAATATTGTGAAGCAGACAATAAATAAAAATTATGCCGAT
  GATCAAACATATTATTTAAGTGGTATGATTATGGCTACTATCAATTCTAAGTCCGAAGGT
  ATGACAGAATGGGAGCGAGGACTTAGAATGTTTCCTAAATCAGGTTTATTAAATTTTGAG
  TTAGCGATAGCAAATCGTTCATTAAATGATGATGAAAAAGCATTAAAATATGTGCGTAAA
  GCATTAAATGCAGACCCTAAAAATACAGATTATATTAACTTAGAAAAAGAGTTGACTAAA
  TCAAATGAGTCGAAAAATAAATAA
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  1464
  

⊟Protein

⊟General

• locus tag: NWMN_RS08195 [old locus tag: NWMN_1452 ]
• symbol: NWMN_RS08195
• description: rhomboid family intramembrane serine protease
• length: 487
• theoretical pI: 9.54533
• theoretical MW: 56889.1
• GRAVY: 0.0568788

⊟Function

• TIGRFAM:
  rhomboid family protease GlpG (TIGR04239; EC 3.4.21.105; HMM-score: 54.7)
  and 3 more
  rhombosortase (TIGR03902; EC 3.4.21.-; HMM-score: 42.9)
  putative PEP-CTERM system TPR-repeat lipoprotein (TIGR02917; HMM-score: 20.7)
  type IV pilus biogenesis/stability protein PilW (TIGR02521; HMM-score: 17.2)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  Rhomboid-like (CL0207) Rhomboid; Rhomboid family (PF01694; HMM-score: 119.3)
  and 8 more
  TPR (CL0020) Fis1_TPR_C; Fis1 C-terminal tetratricopeptide repeat (PF14853; HMM-score: 20.6)
  TPR_2; Tetratricopeptide repeat (PF07719; HMM-score: 20.3)
  TPR_8; Tetratricopeptide repeat (PF13181; HMM-score: 17)
  TPR_19; Tetratricopeptide repeat (PF14559; HMM-score: 15.7)
  TPR_16; Tetratricopeptide repeat (PF13432; HMM-score: 14.4)
  TPR_1; Tetratricopeptide repeat (PF00515; HMM-score: 13.7)
  no clan defined Sensor_TM1; Sensor N-terminal transmembrane domain (PF13755; HMM-score: 10.2)
  O-anti_assembly (CL0499) Wzy_C; O-Antigen ligase (PF04932; HMM-score: 5.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 7
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.001751
  • TAT(Tat/SPI): 0.000133
  • LIPO(Sec/SPII): 0.000329
• predicted transmembrane helices (TMHMM): 7

⊟Accession numbers

• GI: 446940611 NCBI
• RefSeq: WP_001017867 NCBI
• UniProt: see NWMN_1452

⊟Protein sequence

• MNIDKQFWKTIYYWIRYLNFDIVSREKDDQEIWLAHKRKKQVVIFKQHIKSTQEIRFDKAKVLEHKDEIANFISFEPQSFEFYYFTESEFSEEQLNEVSPIRIKFNVIRHTKDLIKHMPNIFLARLISEDNDKKTYMFYKRKVLTDNFLDKYMQKFSPATYTIIFVNVLIWLCMILYLNNFSDVKLLDVGGLVHFNVVHGEWYRIVTSMFLHFSFEHILMNMLSLFIFGKIVEAIIGSWRMLTVYFIAGLFGNFVSLSFNTTTISVGASGAIFGLIGSIFAMMYVSKTFNKKMLGQLLIALVILVGVSLFMSNINIVAHIGGFIGGLLITLIGYYYKVNRNIFWILLIGMLVIFIALQIRIFTIKEDNIYNKLIKDDMTSGNYDNAQNIVKQTINKNYADDQTYYLSGMIMATINSKSEGMTEWERGLRMFPKSGLLNFELAIANRSLNDDEKALKYVRKALNADPKNTDYINLEKELTKSNESKNK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications