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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS08200 [old locus tag: NWMN_1453 ]
  • pan locus tag?: SAUPAN004131000
  • symbol: NWMN_RS08200
  • pan gene symbol?: —
  • synonym:
  • product: 5-formyltetrahydrofolate cyclo-ligase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_RS08200 [old locus tag: NWMN_1453 ]
  • symbol: NWMN_RS08200
  • product: 5-formyltetrahydrofolate cyclo-ligase
  • replicon: chromosome
  • strand: -
  • coordinates: 1620732..1621271
  • length: 540
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_009641 (1620732..1621271) NCBI
  • BioCyc:
  • MicrobesOnline: see NWMN_1453

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    GTGACTAAAAATGAGATTAGAAAATACATTTTACATAAAATGAAGAATTTTAATAAAGCT
    GAAAAGCGAAAAGCAGACACATGGTTAAGAAATCAATTTTTTGCAACTGAAGAATACAAA
    GAAGCAAACGCAATTGCGCTAGTTCTTTCTTTTAATCATGAAGTAGATACTTTTTCTATT
    ATTGAACAAGCCTTAATGGATCATAAACGTATTTTTGTACCGAAAATGGATTATTTAAAT
    CATCAAATGACTTTTAAAGAGATATTTAATCTCAAAGATATTGATGTCGATAATAAGGGG
    ATTTACTATCCAACTTCAAAAGGTGAAACAACGAATAACCTAGATTTAATTGTTGTTCCT
    GGTGTTGGATTTCAAGACGATGGATATAGAATTGGGTATGGTGGTGGCTATTACGACAGG
    TTTTTAGCTAATTATCAGACAAAGACAATAAGCTTATTATACGATTTTCAAATAACATCA
    TTTGAACCGGAATCATTCGATCAACCAGTCGATAAATTGATTATATATCAAGCAGCATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_RS08200 [old locus tag: NWMN_1453 ]
  • symbol: NWMN_RS08200
  • description: 5-formyltetrahydrofolate cyclo-ligase
  • length: 179
  • theoretical pI: 6.12385
  • theoretical MW: 20999.7
  • GRAVY: -0.479888

⊟Function[edit | edit source]

  • reaction:
    EC 6.3.3.2?  ExPASy
    5-formyltetrahydrofolate cyclo-ligase ATP + 5-formyltetrahydrofolate = ADP + phosphate + 5,10-methenyltetrahydrofolate
  • TIGRFAM:
    Metabolism Central intermediary metabolism One-carbon metabolism 5-formyltetrahydrofolate cyclo-ligase (TIGR02727; EC 6.3.3.2; HMM-score: 160.7)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    ISOCOT_Fold (CL0246) 5-FTHF_cyc-lig; 5-formyltetrahydrofolate cyclo-ligase family (PF01812; HMM-score: 128.3)
    and 1 more
    Rep (CL0169) PV_NSP1; Parvovirus non-structural protein 1 (PF12433; HMM-score: 16.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Mg2+
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.32
    • Cytoplasmic Membrane Score: 9.55
    • Cellwall Score: 0.12
    • Extracellular Score: 0.01
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002398
    • TAT(Tat/SPI): 0.000128
    • LIPO(Sec/SPII): 0.000361
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTKNEIRKYILHKMKNFNKAEKRKADTWLRNQFFATEEYKEANAIALVLSFNHEVDTFSIIEQALMDHKRIFVPKMDYLNHQMTFKEIFNLKDIDVDNKGIYYPTSKGETTNNLDLIVVPGVGFQDDGYRIGYGGGYYDRFLANYQTKTISLLYDFQITSFEPESFDQPVDKLIIYQAA

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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