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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS08635 [old locus tag: NWMN_1539 ]
  • pan locus tag?: SAUPAN004243000
  • symbol: NWMN_RS08635
  • pan gene symbol?: secDF
  • synonym:
  • product: protein translocase subunit SecDF

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_RS08635 [old locus tag: NWMN_1539 ]
  • symbol: NWMN_RS08635
  • product: protein translocase subunit SecDF
  • replicon: chromosome
  • strand: -
  • coordinates: 1706581..1708860
  • length: 2280
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_009641 (1706581..1708860) NCBI
  • BioCyc:
  • MicrobesOnline: see NWMN_1539

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
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    481
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    661
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    841
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    1021
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    1321
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    1621
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    2041
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    2221
    GTGAAGAAAAGTAGTAGAATAATTGCGTTCTTGCTTTTAGTCGTATTGTTGTTTGCCGGA
    ATGGCTGCAACTTATAAAAGTGTTATAAAGAATGTCAATTTAGGACTTGATTTGCAAGGT
    GGATTTGAGGTGCTTTATCAAGTCGATCCTTTAAATAAAGGCGACAAAATAGATAAAAAA
    GCCTTACAATCAACAGCGCAAACATTAGAAAATCGTGTTAACGTTTTAGGTGTTTCGGAA
    CCTAAAATTCAAGTTGAAGAACCTAATAGAATTAGAGTACAACTTGCTGGTGTAACAGAC
    CAGAATGAAGCACGTAAAATTTTATCTTCTCAAGCTAATTTAACAATTCGCGATGCAGAA
    GATAAAGTAAAATTAAGCGGTTCTGATATTAAACAGGGATCTGCCAAACAAGAATTTAAA
    CAGGAAACAAACCAGCCTACAGTTACTTTTAAAGTAAAAGACAAAAACAAATTTAAAAAA
    GTAACGGAAGAAATCTCTAAGAAACGTGATAATGTCATGGTTGTTTGGTTAGATTTCAAA
    AAAGGTGACAGCTATAAAAAAGAAGCACAAAAGAAAAACCCTAAATTTATTTCTGCAGCA
    AGTGTTGATCAACCAATCAATTCTGATAGCGTAGAAATCTCGGGTGGATTTAAAGGACAA
    GAAGGCGTTAAAAAAGCAAAACAAATCGCCGAATTGTTAAATGCAGGTTCTTTACCAGTT
    GACTTGAAAGAAATTTATTCAAACTCAGTAGGTGCCCAATTTGGTCAAGATGCATTAGAT
    AAAACTGTGTTTGCATCGTTTATTGGTGTAGCATTAATTTATTTATTCATGCTTGGATTC
    TACCGATTACCTGGTTTAGTAGCGATTATTGCATTGACAACTTATATCTATCTAACGTTA
    GTAGCATTTAATTTCATTTCCGGGGTTCTAACTTTACCAGGATTAGCGGCGTTGGTATTA
    GGTGTAGGTATGGCTGTAGATGCCAATATTATCATGTATGAGCGTATTAAGGATGAACTT
    CGAATAGGTAGAACGATAAAGCAAGCCTTTTCTAAAGCAAACAAAAGTTCATTCCTAACA
    ATTTTTGATTCTAACTTAACAACAGTTATCGCCGCAGCAGTATTATTCTTCTTCGGTGAA
    AGTTCAGTTAAAGGTTTCGCGACAATGTTATTATTAGGTATTCTAATGATCTTTGTTACA
    GCCGTGTTCTTATCAAGATTCTTATTATCATTACTTGTTTCATCAAATATATTCAAAAAT
    CAATTTTGGTTATTTGGTGTTAAAAAGAATAAACGACATGATATTAATGAAGGTGTAGAT
    GTTCATGACCTTAAAACTTCATTCGAGAAATGGAATTTTGTTAAATTAGCTAAGCCATTA
    ATTGGAGTAAGTATTTTAATTGTAGTTGTCGGTTTAGTTATTCTTTATATCTTCAAGTTA
    AACTTAGGAATTGATTTCTCAAGTGGTACACGCGTAGATTTCCAATCTAAACAAGCAATT
    ACTCAACAAAAGGTAGAGCAAGTTGTAAAAGATAGTGGACTTAAAGCGGATCAAATTCAG
    ATCAATGGTAAAGACAATAAAGTAGCAACTGTTCAATTTAAAGACGATTTAACGCGTGCT
    CAAGATAATAAATTGAGTGATAATATTAAATCGAAATTCGGTGACACACCTCAAATTAAC
    ACTGTATCACCAATTATTGGACAGGAATTAGCAAAAAATGCAATGCTTGCATTAATCTAT
    GCATCGATAGGTATCATCATCTATGTATCATTACGATTTGAATGGCGCATGGGTCTTTCA
    TCTGTATTGGCATTATTACATGATGTATTCATTATAGTAGCGATTTTCAGTTTATTTAGA
    ATTGAAGTAGATTTAACATTTATCGCCGCTGTATTAACAATTGTCGGTTATTCAATTAAT
    GATACAATCGTAACGTTTGACCGTGTACGTGAAAACTTACAAAAGGTTAAAGTGATTACG
    ACAACAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCA
    ATTAATACAGTATTAACAGTTATTGTAGTAGTAGTTGCTATACTATTCTTCGGTGCTCCT
    ACGATATTCAACTTTACTTTAGCATTATTTATCGGATTGATTTCTGGTGTATTCTCTTCA
    ATCTTCATTGCCGTTCCGCTATGGGGAATAATGAAAAAACGTCAGTTGAAAAAATCGCCG
    AAACACAAATTAGTTGTATATAAAGAAAAGAAATCGAACGATGAAAAGATTTTAGTTTAA
    60
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    2160
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    2280

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_RS08635 [old locus tag: NWMN_1539 ]
  • symbol: NWMN_RS08635
  • description: protein translocase subunit SecDF
  • length: 759
  • theoretical pI: 10.3191
  • theoretical MW: 84253.3
  • GRAVY: 0.234124

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Protein fate Protein and peptide secretion and trafficking protein-export membrane protein SecD (TIGR01129; HMM-score: 386.9)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking protein-export membrane protein, SecD/SecF family (TIGR00916; HMM-score: 338.2)
    and 3 more
    Genetic information processing Protein fate Protein and peptide secretion and trafficking protein-export membrane protein SecF (TIGR00966; HMM-score: 276.2)
    Metabolism Transport and binding proteins Unknown substrate efflux transporter, putative, hydrophobe/amphiphile efflux-3 (HAE3) family (TIGR00921; HMM-score: 58.2)
    Metabolism Transport and binding proteins Unknown substrate transport protein (TIGR00833; HMM-score: 27.2)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    RND_permease (CL0322) SecD_SecF; Protein export membrane protein (PF02355; HMM-score: 256)
    and 6 more
    no clan defined Sec_GG; SecD/SecF GG Motif (PF07549; HMM-score: 39.6)
    RND_permease (CL0322) MMPL; MMPL family (PF03176; HMM-score: 39.2)
    no clan defined Selenoprotein_S; Selenoprotein S (SelS) (PF06936; HMM-score: 12.7)
    POTRA (CL0191) POTRA; Surface antigen variable number repeat (PF07244; HMM-score: 12.4)
    no clan defined SecD-TM1; SecD export protein N-terminal TM region (PF13721; HMM-score: 11.4)
    BRCT-like (CL0459) RTT107_BRCT_6; Regulator of Ty1 transposition protein 107 BRCT domain (PF16771; HMM-score: 8.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 12
  • LocateP:
  • SignalP: Signal peptide SP(Sec/SPI) length 22 aa
    • SP(Sec/SPI): 0.427618
    • TAT(Tat/SPI): 0.00166
    • LIPO(Sec/SPII): 0.373685
    • Cleavage Site: CS pos: 22-23. GMA-AT. Pr: 0.1458
  • predicted transmembrane helices (TMHMM): 12

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKKSSRIIAFLLLVVLLFAGMAATYKSVIKNVNLGLDLQGGFEVLYQVDPLNKGDKIDKKALQSTAQTLENRVNVLGVSEPKIQVEEPNRIRVQLAGVTDQNEARKILSSQANLTIRDAEDKVKLSGSDIKQGSAKQEFKQETNQPTVTFKVKDKNKFKKVTEEISKKRDNVMVVWLDFKKGDSYKKEAQKKNPKFISAASVDQPINSDSVEISGGFKGQEGVKKAKQIAELLNAGSLPVDLKEIYSNSVGAQFGQDALDKTVFASFIGVALIYLFMLGFYRLPGLVAIIALTTYIYLTLVAFNFISGVLTLPGLAALVLGVGMAVDANIIMYERIKDELRIGRTIKQAFSKANKSSFLTIFDSNLTTVIAAAVLFFFGESSVKGFATMLLLGILMIFVTAVFLSRFLLSLLVSSNIFKNQFWLFGVKKNKRHDINEGVDVHDLKTSFEKWNFVKLAKPLIGVSILIVVVGLVILYIFKLNLGIDFSSGTRVDFQSKQAITQQKVEQVVKDSGLKADQIQINGKDNKVATVQFKDDLTRAQDNKLSDNIKSKFGDTPQINTVSPIIGQELAKNAMLALIYASIGIIIYVSLRFEWRMGLSSVLALLHDVFIIVAIFSLFRIEVDLTFIAAVLTIVGYSINDTIVTFDRVRENLQKVKVITTTEQIDDIVNRSIRQTMTRSINTVLTVIVVVVAILFFGAPTIFNFTLALFIGLISGVFSSIFIAVPLWGIMKKRQLKKSPKHKLVVYKEKKSNDEKILV

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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