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NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS09215 [old locus tag: NWMN_1641 ]
  • pan locus tag?: SAUPAN004425000
  • symbol: NWMN_RS09215
  • pan gene symbol?: trmB
  • synonym:
  • product: tRNA (guanosine(46)-N7)-methyltransferase TrmB

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_RS09215 [old locus tag: NWMN_1641 ]
  • symbol: NWMN_RS09215
  • product: tRNA (guanosine(46)-N7)-methyltransferase TrmB
  • replicon: chromosome
  • strand: -
  • coordinates: 1828673..1829317
  • length: 645
  • essential: unknown other strains

Accession numbers[edit | edit source]

  • Location: NC_009641 (1828673..1829317) NCBI
  • BioCyc:
  • MicrobesOnline: see NWMN_1641

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGAGAGTTCGATACAAACCATGGGCTAAAGATTATTTAAAAGATCATCCTGAATTGGTT
    GACATGGACGGTCAACATGCAGGGAAAATGACTGAATGGTTTGATAAAACGCAACCGATA
    CACATTGAAATTGGTTCGGGTATGGGGCAGTTTATTACAACATTAGCTGCTCAAAATCCT
    CATATTAACTATATTTCAATGGAACGTGAGAAAAGTATCGTTTATAAAGTATTGGATAAA
    GTTAAAGAGATGGGTTTAACAAATTTAAAGATTATTTGTAATGACGCCATTGAATTAAAT
    GAATATTTTAAAGACGGCGAAGTTTCGCGTATTTATTTGAACTTTTCAGATCCATGGCCA
    AAAAATCGTCATGCAAAACGACGTTTAACGTATCATACGTTTTTAGCGTTGTATCAACAA
    ATCTTAAATGATGAAGGAGATTTACATTTTAAAACTGATAACAGAGGACTATTTGCCTAC
    AGTTTAGAAAGTATGTCTCAATTTGGAATGTATTTTACAAAAATAAATCTAAATTTACAT
    CAAGAAGACGATGGCAGTAATATTTTAACAGAATATGAAAAGAAATTTTCGGATAAAGGG
    TCACGTATTTATCGTATGGAAGCGAAATTCCATTCACAAAAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    645

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: NWMN_RS09215 [old locus tag: NWMN_1641 ]
  • symbol: NWMN_RS09215
  • description: tRNA (guanosine(46)-N7)-methyltransferase TrmB
  • length: 214
  • theoretical pI: 7.82983
  • theoretical MW: 25274.6
  • GRAVY: -0.709813

Function[edit | edit source]

  • reaction:
    EC 2.1.1.33?  ExPASy
    tRNA (guanine46-N7)-methyltransferase S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA
  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA (guanine-N(7)-)-methyltransferase (TIGR00091; EC 2.1.1.33; HMM-score: 184.4)
    and 4 more
    Genetic information processing Protein synthesis tRNA and rRNA base modification ribosomal RNA small subunit methyltransferase A (TIGR00755; EC 2.1.1.182; HMM-score: 16.1)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Biotin malonyl-acyl carrier protein O-methyltransferase BioC (TIGR02072; EC 2.1.1.-; HMM-score: 14.9)
    Genetic information processing Protein fate Protein modification and repair protein-L-isoaspartate O-methyltransferase (TIGR00080; EC 2.1.1.77; HMM-score: 13.8)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin precorrin-6Y C5,15-methyltransferase (decarboxylating), CbiT subunit (TIGR02469; EC 2.1.1.132; HMM-score: 13.2)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    NADP_Rossmann (CL0063) Methyltransf_4; Putative methyltransferase (PF02390; HMM-score: 169.6)
    and 7 more
    DUF938; Protein of unknown function (DUF938) (PF06080; HMM-score: 17.9)
    Methyltransf_25; Methyltransferase domain (PF13649; HMM-score: 16.2)
    PCMT; Protein-L-isoaspartate(D-aspartate) O-methyltransferase (PCMT) (PF01135; HMM-score: 15.4)
    Methyltransf_31; Methyltransferase domain (PF13847; HMM-score: 15.2)
    Methyltransf_12; Methyltransferase domain (PF08242; HMM-score: 13.3)
    Methyltr_RsmB-F; 16S rRNA methyltransferase RsmB/F (PF01189; HMM-score: 12.2)
    GidB; rRNA small subunit methyltransferase G (PF02527; HMM-score: 12)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.007362
    • TAT(Tat/SPI): 0.000343
    • LIPO(Sec/SPII): 0.00066
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MRVRYKPWAKDYLKDHPELVDMDGQHAGKMTEWFDKTQPIHIEIGSGMGQFITTLAAQNPHINYISMEREKSIVYKVLDKVKEMGLTNLKIICNDAIELNEYFKDGEVSRIYLNFSDPWPKNRHAKRRLTYHTFLALYQQILNDEGDLHFKTDNRGLFAYSLESMSQFGMYFTKINLNLHQEDDGSNILTEYEKKFSDKGSRIYRMEAKFHSQK

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]