PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_RS09335 [old locus tag: NWMN_1663 ]
• pan locus tag^?: SAUPAN004460000
• symbol: NWMN_RS09335
• pan gene symbol^?: —
• synonym:
• product: pyridine nucleotide-disulfide oxidoreductase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_RS09335 [old locus tag: NWMN_1663 ]
• symbol: NWMN_RS09335
• product: pyridine nucleotide-disulfide oxidoreductase
• replicon: chromosome
• strand: -
• coordinates: 1859282..1860784
• length: 1503
• essential: unknown other strains

⊟Accession numbers

• Location: NC_009641 (1859282..1860784) NCBI
• BioCyc:
• MicrobesOnline: see NWMN_1663

⊟Phenotype

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⊟DNA sequence

• 1
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  ATGCGTGTAGCAATAATAGGCATGGGAACTGCTGGTGTAAGTGTATTGAGAGAGTTAGTT
  AAGCACCCGAAGTTTAATCAATTAGATATAGATTTATACGATGATAAAGTAAATATGGGA
  CAGGGCGTACCATTTCAAAATGATAGTTCTGAGCTGCTAATTAATATGCCTTCGAAAAAG
  ATGAGTTTAAATTTAGATGATGAGACTGAATTTTGGAAGTGGTATAAACAACAAACTGAT
  TTTAATTTTGATGAACCTGCATATTTACCGAGATTTGTATTCGGACATTATATGAAATCT
  TATTTATCTATGTTCACGAAAAAATATCCAAATATATCAACTAATTATAATAAAGTTCAA
  GAGATTTATACAAATTCTAATATCGATGAAACGAATTTAACTTATTACATATGTACTACA
  AATTCAGGACAATCATGGCAAGCGTACGATTATGTATTTTTAACATGTGGTACATTTGCC
  TACCATGATCCATATAACTTAAAGGGTAAAAAAGGATATATCGCTACGCCTTATCCAACA
  TATAATACGTTAGACGAAGTAAATGAACTGGACGATATTGCGATTATTGGTACAGGCCTT
  GCCAGTTTAGATGTTGTCCGTTATGTTGCTGCTCATCATCCAAAATTACCAATTACTATG
  ACGAGTCGTTCTGCTCATTTACCAAGTGTAAGAGGTACAATGATAGACGTGACATTCAAG
  TACTTAACTAAAGACAAATTAAATGACATTAAAAAACATCATTTCGGCAATGCACCGCTT
  GATACTTTAGTTTCTTTATTTTTAAAAGAATGTGCTGATTATGACATTGATTTTAAAAAA
  TTAGTACATAGACGTACAGGAAATCATATTGCAGATTTGAAGTATGATTTAGCGCGTCCA
  ACAGAAATGGGCATTTTCCAAAGTATGATTGAACATTTGAAAGAGAATTTAAATTGGATA
  TGGAATAGCTTAAGTATTGAAGACCAACATCAATTTAATCAAAAATACTCAAAGATGATT
  CAATTAAATTCAAATCCAATGCCTCCAAGAACTGCTGAACTAATTATTGAATTAATAGAA
  AATAAGTCTTTAATTTTGAAAAAAGATTTAGAAGATGTGAAACATGATGGCAAGTTGTAT
  TATTTTTCATACAAAAACCAAGAATCAGTAGACATATATAATGTTGTCATTAACGCGACA
  GGTGCAAAAAGTCATTTAAATGAATTGGATCAAGATGATCAATTAATTAGAAATCTTGAA
  AATAGACAAATTGTACAAGCACACCCAATGGGAGGCATACAAATCATTCCGGAAACCAAT
  CAAGTTATTAGTCCAAGGTTTGGAACATTAACAAATATGATTGCGATAGGTCAAATGACA
  AACGGTGTTAATAAACTACGAAACGGTGTGAAAATGATTGTAGAACAAGTTGCGCATACA
  GTATCTCAATTATATGATGCTTTAGAATCGAATGAGCAACAACAGCGCAGTGACAATCAA
  TAA
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⊟Protein

⊟General

• locus tag: NWMN_RS09335 [old locus tag: NWMN_1663 ]
• symbol: NWMN_RS09335
• description: pyridine nucleotide-disulfide oxidoreductase
• length: 500
• theoretical pI: 6.41197
• theoretical MW: 57563
• GRAVY: -0.475

⊟Function

• TIGRFAM:
  Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA U-34 5-methylaminomethyl-2-thiouridine biosynthesis protein MnmC, C-terminal domain (TIGR03197; HMM-score: 15.4)
  salicylate 1-monooxygenase (TIGR03219; EC 1.14.13.1; HMM-score: 14.9)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  NADP_Rossmann (CL0063) NAD_binding_9; FAD-NAD(P)-binding (PF13454; HMM-score: 113.8)
  and 6 more
  Pyr_redox_2; Pyridine nucleotide-disulphide oxidoreductase (PF07992; HMM-score: 32.6)
  Pyr_redox_3; Pyridine nucleotide-disulphide oxidoreductase (PF13738; HMM-score: 24.8)
  K_oxygenase; L-lysine 6-monooxygenase (NADPH-requiring) (PF13434; HMM-score: 20.1)
  DAO; FAD dependent oxidoreductase (PF01266; HMM-score: 17)
  Thi4; Thi4 family (PF01946; HMM-score: 15)
  Sacchrp_dh_NADP; Saccharopine dehydrogenase NADP binding domain (PF03435; HMM-score: 13)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.016499
  • TAT(Tat/SPI): 0.000305
  • LIPO(Sec/SPII): 0.005268
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 447184421 NCBI
• RefSeq: WP_001261677 NCBI
• UniProt: see NWMN_1663

⊟Protein sequence

• MRVAIIGMGTAGVSVLRELVKHPKFNQLDIDLYDDKVNMGQGVPFQNDSSELLINMPSKKMSLNLDDETEFWKWYKQQTDFNFDEPAYLPRFVFGHYMKSYLSMFTKKYPNISTNYNKVQEIYTNSNIDETNLTYYICTTNSGQSWQAYDYVFLTCGTFAYHDPYNLKGKKGYIATPYPTYNTLDEVNELDDIAIIGTGLASLDVVRYVAAHHPKLPITMTSRSAHLPSVRGTMIDVTFKYLTKDKLNDIKKHHFGNAPLDTLVSLFLKECADYDIDFKKLVHRRTGNHIADLKYDLARPTEMGIFQSMIEHLKENLNWIWNSLSIEDQHQFNQKYSKMIQLNSNPMPPRTAELIIELIENKSLILKKDLEDVKHDGKLYYFSYKNQESVDIYNVVINATGAKSHLNELDQDDQLIRNLENRQIVQAHPMGGIQIIPETNQVISPRFGTLTNMIAIGQMTNGVNKLRNGVKMIVEQVAHTVSQLYDALESNEQQQRSDNQ

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: SigB see NWMN_1663

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications