PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_RS09565 [old locus tag: NWMN_1702 ]
• pan locus tag^?: SAUPAN004561000
• symbol: NWMN_RS09565
• pan gene symbol^?: splE
• synonym:
• product: serine protease SplE

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_RS09565 [old locus tag: NWMN_1702 ]
• symbol: NWMN_RS09565
• product: serine protease SplE
• replicon: chromosome
• strand: -
• coordinates: 1900155..1900871
• length: 717
• essential: unknown other strains

⊟Accession numbers

• Location: NC_009641 (1900155..1900871) NCBI
• BioCyc:
• MicrobesOnline: see NWMN_1702

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGAATAAAAATATAATCATCAAAAGTATTGCAGCATTGACGATTTTAACATCAGTGACT
  GGCGTCGGCACAACAGTGGTTGAGGGTATTCAACAAACGGCTAAAGCTGAACATAATGTG
  AAACTAATCAAAAATACTAATGTAGCACCATACAATGGTGTCGTTTCGATAGGATCTGGA
  ACAGGTTTCATTGTCGGTAAAAATACAATTGTTACCAACAAGCATGTCGTTGCAGGTATG
  GAAATTGGTGCACATATTATAGCGCATCCCAATGGTGAATATAATAATGGCGGATTTTAT
  AAAGTTAAAAAAATTGTCCGTTATTCAGGTCAAGAAGATATTGCCATTCTACATGTGGAA
  GATAAAGCTGTTCATCCAAAAAACAGGAATTTTAAAGATTACACAGGCATTTTAAAAATA
  GCATCAGAAGCTAAAGAAAATGAACGCATTTCAATTGTTGGCTATCCAGAACCATATATA
  AATAAATTTCAAATGTATGAGTCAACAGGAAAAGTGCTGTCAGTTAAAGGCAACATGATT
  ATTACTGATGCTTTCGTAGAACCAGGCAACTCAGGTTCAGCTGTATTTAACAGTAAATAC
  GAAGTTGTAGGTGTTCACTTTGGTGGAAACGGCCCTGGAAATAAAAGTACAAAAGGATAT
  GGTGTTTATTTCTCTCCTGAAATTAAGAAATTCATTGCAGATAACACAGATAAATAA
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  717
  

⊟Protein

⊟General

• locus tag: NWMN_RS09565 [old locus tag: NWMN_1702 ]
• symbol: NWMN_RS09565
• description: serine protease SplE
• length: 238
• theoretical pI: 9.81932
• theoretical MW: 25679.1
• GRAVY: -0.195378

⊟Function

• reaction:
  EC 3.4.21.-?  ExPASy
• TIGRFAM:
  Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides peptidase Do (TIGR02037; EC 3.4.21.-; HMM-score: 35)
  Genetic information processing Protein fate Protein folding and stabilization peptidase Do (TIGR02037; EC 3.4.21.-; HMM-score: 35)
  and 2 more
  Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides periplasmic serine peptidase DegS (TIGR02038; EC 3.4.21.-; HMM-score: 24.8)
  Signal transduction Regulatory functions Protein interactions periplasmic serine peptidase DegS (TIGR02038; EC 3.4.21.-; HMM-score: 24.8)
• TheSEED: data available for COL, NCTC8325, USA300_FPR3757
• PFAM:
  Peptidase_PA (CL0124) Trypsin; Trypsin (PF00089; HMM-score: 71.6)
  Trypsin_2; Trypsin-like peptidase domain (PF13365; HMM-score: 70.6)
  and 4 more
  Peptidase_S7; Peptidase S7, Flavivirus NS3 serine protease (PF00949; HMM-score: 24.6)
  Peptidase_S46; Peptidase S46 (PF10459; HMM-score: 24.6)
  Peptidase_S32; Equine arteritis virus serine endopeptidase S32 (PF05579; HMM-score: 18.5)
  DUF31; Putative peptidase (DUF31) (PF01732; HMM-score: 15.9)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Extracellular
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0.09
  • Cellwall Score: 0.18
  • Extracellular Score: 9.73
  • Internal Helices: 0
• LocateP:
• SignalP: Signal peptide SP(Sec/SPI) length 36 aa
  • SP(Sec/SPI): 0.930491
  • TAT(Tat/SPI): 0.005732
  • LIPO(Sec/SPII): 0.026139
  • Cleavage Site: CS pos: 36-37. AKA-EH. Pr: 0.6736
• predicted transmembrane helices (TMHMM): 1

⊟Accession numbers

• GI: 446961503 NCBI
• RefSeq: WP_001038759 NCBI
• UniProt: see NWMN_1702

⊟Protein sequence

• MNKNIIIKSIAALTILTSVTGVGTTVVEGIQQTAKAEHNVKLIKNTNVAPYNGVVSIGSGTGFIVGKNTIVTNKHVVAGMEIGAHIIAHPNGEYNNGGFYKVKKIVRYSGQEDIAILHVEDKAVHPKNRNFKDYTGILKIASEAKENERISIVGYPEPYINKFQMYESTGKVLSVKGNMIITDAFVEPGNSGSAVFNSKYEVVGVHFGGNGPGNKSTKGYGVYFSPEIKKFIADNTDK

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• data available for NCTC8325

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications