PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_RS11880 [old locus tag: NWMN_2057 ]
• pan locus tag^?: SAUPAN005495000
• symbol: NWMN_RS11880
• pan gene symbol^?: mtlA
• synonym:
• product: PTS mannitol transporter subunit IICB

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_RS11880 [old locus tag: NWMN_2057 ]
• symbol: NWMN_RS11880
• product: PTS mannitol transporter subunit IICB
• replicon: chromosome
• strand: +
• coordinates: 2277196..2278734
• length: 1539
• essential: unknown other strains

⊟Accession numbers

• Location: NC_009641 (2277196..2278734) NCBI
• BioCyc:
• MicrobesOnline: see NWMN_2057

⊟Phenotype

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⊟DNA sequence

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  ATGTCACAAACTGAAGAGAAAAAAGGAATTGGTCGTCGTGTTCAAGCATTTGGATCATTC
  TTAAGTAGTATGATCATGCCAAACATTGGCGCTTTCATCGCTTGGGGTTTTATCGCGGCC
  ATTTTTATAGATAATGGATGGTTACCTAACAAAGATTTAGCTACTTTAGCAGGACCAATG
  ATTACTTATTTAATCCCATTATTGATCGCATTTAGTGGTGGTCGTTTAATTTATGATTTA
  CGTGGTGGTATCATCGCAGCAACAGCAACTATGGGGGTCATCGTTGCATTACCTGATACA
  CCAATGTTACTTGGTGCAATGATTATGGGTCCACTTGTTGGTTGGTTAATGAAGAAGACC
  GACCAATTGATTCAACCTAGAACGCCGCAAGGTTTTGAAATGTTATTTAATAACTTTTCT
  GCTGGTATTTTAGGATTTATCATGACGATTGCAGGATTTAAAATTTTAGCACCACTTATG
  AAATTTATTATGCATATTTTATCAGTGGCAGTTGAAGCATTAGTACATGCACACCTACTT
  CCACTTGTAAGTATTTTAGTAGAGCCTGCAAAAATTGTATTTTTAAACAATGCGATTAAC
  CATGGTGTATTCACACCGCTTGGTGCAGATCAAGCTGCAAAAGCTGGTCAATCAATTTTA
  TACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTT
  GGAAAAGGCACTGCAAAAGCAACGTCATATGGTGCTGGAATTATCCACTTCTTAGGTGGT
  ATTCATGAAATTTATTTCCCATATGTATTAATGCGTCCTTTATTATTTATCGCTGTTATT
  TTAGGTGGAATGACTGGTGTTGCAACTTATCAAGCAACAGGATTTGGATTTAAAAGTCCC
  GCGTCACCAGGTTCATTTATAGTTTACTGTTTAAATGCACCTAGAGGTGAATTCTTGCAC
  ATGTTACTCGGTGTCTTCCTTGCTGCACTTGTATCATTCGTTGTAGCTGCTTTAATTATG
  AAGTTCACTAGAGAACCAAAGCAGGATTTAGAAGCTGCGACAGCTCAAATGGAAAATACT
  AAAGGGAAAAAATCAAGCGTTGCTTCTAAGTTAGTATCTTCTGATAAAAATGTTAATACA
  GAAGAAAATGCTAGTGGTAATGTTAGTGAAACATCTTCATCAGATGATGATCCTGAAGCG
  CTATTGGATAATTACAACACTGAAGATGTTGATGCACACAATTACAATAATATAAATCAT
  GTTATTTTTGCCTGCGATGCGGGTATGGGTTCTAGTGCAATGGGTGCAAGCATGTTACGT
  AATAAATTTAAAAAGGCGGGCATTAATGATATTACAGTTACAAATACTGCGATTAATCAA
  TTGCCAAAAGATGCTCAATTAGTTATCACTCAGAAAAAACTAACTGATCGTGCTATTAAA
  CAAACACCAAATGCCATCCATATTTCAGTGGATAATTTCCTTAATTCACCAAGATATGAA
  GAACTTTTAAATAATCTAAAAAAAGATGATCAAGCATAA
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⊟Protein

⊟General

• locus tag: NWMN_RS11880 [old locus tag: NWMN_2057 ]
• symbol: NWMN_RS11880
• description: PTS mannitol transporter subunit IICB
• length: 512
• theoretical pI: 7.76606
• theoretical MW: 55064.9
• GRAVY: 0.299219

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, mannitol-specific IIC component (TIGR00851; EC 2.7.1.69; HMM-score: 589)
  Signal transduction Signal transduction PTS PTS system, mannitol-specific IIC component (TIGR00851; EC 2.7.1.69; HMM-score: 589)
  and 2 more
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, lactose/cellobiose family IIB component (TIGR00853; HMM-score: 14.9)
  Signal transduction Signal transduction PTS PTS system, lactose/cellobiose family IIB component (TIGR00853; HMM-score: 14.9)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  PTS_EIIC (CL0493) PTS_EIIC; Phosphotransferase system, EIIC (PF02378; HMM-score: 60.2)
  and 3 more
  Phosphatase (CL0031) PTS_IIB; PTS system, Lactose/Cellobiose specific IIB subunit (PF02302; HMM-score: 44)
  Rhodanese; Rhodanese-like domain (PF00581; HMM-score: 16.4)
  no clan defined Ctr; Ctr copper transporter family (PF04145; HMM-score: 13.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 6
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.009005
  • TAT(Tat/SPI): 0.001102
  • LIPO(Sec/SPII): 0.003247
• predicted transmembrane helices (TMHMM): 9

⊟Accession numbers

• GI: 446005952 NCBI
• RefSeq: WP_000083807 NCBI
• UniProt: see NWMN_2057

⊟Protein sequence

• MSQTEEKKGIGRRVQAFGSFLSSMIMPNIGAFIAWGFIAAIFIDNGWLPNKDLATLAGPMITYLIPLLIAFSGGRLIYDLRGGIIAATATMGVIVALPDTPMLLGAMIMGPLVGWLMKKTDQLIQPRTPQGFEMLFNNFSAGILGFIMTIAGFKILAPLMKFIMHILSVAVEALVHAHLLPLVSILVEPAKIVFLNNAINHGVFTPLGADQAAKAGQSILYTIESNPGPGLGILLAYMIFGKGTAKATSYGAGIIHFLGGIHEIYFPYVLMRPLLFIAVILGGMTGVATYQATGFGFKSPASPGSFIVYCLNAPRGEFLHMLLGVFLAALVSFVVAALIMKFTREPKQDLEAATAQMENTKGKKSSVASKLVSSDKNVNTEENASGNVSETSSSDDDPEALLDNYNTEDVDAHNYNNINHVIFACDAGMGSSAMGASMLRNKFKKAGINDITVTNTAINQLPKDAQLVITQKKLTDRAIKQTPNAIHISVDNFLNSPRYEELLNNLKKDDQA

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: MtlR* see NWMN_2057

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications