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NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS12045 [old locus tag: NWMN_2079 ]
  • pan locus tag?: SAUPAN005569000
  • symbol: NWMN_RS12045
  • pan gene symbol?: sfnaC
  • synonym:
  • product: alanine racemase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_RS12045 [old locus tag: NWMN_2079 ]
  • symbol: NWMN_RS12045
  • product: alanine racemase
  • replicon: chromosome
  • strand: -
  • coordinates: 2315620..2316690
  • length: 1071
  • essential: unknown other strains

Accession numbers[edit | edit source]

  • Location: NC_009641 (2315620..2316690) NCBI
  • BioCyc:
  • MicrobesOnline: see NWMN_2079

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGGCACACGTTAACATAAATATATCGAAGATTAAATATAACGCCAAAGTACTTCAAACA
    GTTTTTCAAAGTAAAAATATGCAATTCACACCAGTAATTAAGTGCATAGCTGGTGACCGT
    ACAATTGTAGAAAGCTTAAAAGCGTTAGGTATCAATCATGTTGCAGAATCCAGATTGGAT
    AACATAATTAGTATTGCAGATCAGGATTTAACATATACGTTATTGCGTACGCCTGCCAAA
    AAAGAGATTTCAGATATGATAGAAAAAGTTGATATGAGTATCCAAACAGAATTATCTACC
    ATACATCAAATAAATGAAGTAGCAGAAGTTTTAGGAAAAAAACATAAGATTTTATTAATG
    GTAGATTGGAAAGATGGCCGTGAAGGTGTTCTGACTTATGATGTTTTAGATTATATTAAA
    GAGATTATTCACTTGAAAAATATCCATTTTGTAGGTTTAGCATTCAATTTTATGTGTTTT
    AAATCTGATGCGCCAAGTGATGATGATATCTTTATGATTAATCGATTCGTCTCAGCTGTT
    GAAAGAGAAATAGGATATCGATTGAAAATTATTTCAGGTGGTAATTCGAGCATGTTACCA
    CAACTTTTATATAATGATTTGGGTAAAATAAATGAATTGCGAATAGGTGAAACGTTGTTT
    AGAGGTGTTGATACAACGACAAATCAAGCGATCGCTATGTTATATCAAGATGCGATTACA
    TTGGAGGCTGAGATTTTAGAAATTAAACCTCGAGTTAATACACAAACGCATGAATCATTC
    TTACAAGCTATTGTAGATATTGGTTATTTAGATACAAAAGTCGATAATATTTCACCTATG
    GATCAGCATATCAATATTTTAGGCGCTTCGAGTGATCATTTAATGCTTGATTTAAATGGT
    CAAGGACACTATCAAGTTGGAGATCATATATCTTTTTCATTAAATTATGAAGCACTTTCT
    CATTCGATGTATATGAAAAATTTACATAAGGTATATATTGATGATTCAAAGATTGATACG
    CTACTTCAAAATTTTGATGTGAAATCTCCTGCTATGGTAAACCACTATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1071

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: NWMN_RS12045 [old locus tag: NWMN_2079 ]
  • symbol: NWMN_RS12045
  • description: alanine racemase
  • length: 356
  • theoretical pI: 5.85992
  • theoretical MW: 40348.2
  • GRAVY: -0.0932584

Function[edit | edit source]

  • TIGRFAM:
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan alanine racemase (TIGR00492; EC 5.1.1.1; HMM-score: 32.3)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    TIM_barrel (CL0036) Ala_racemase_N; Alanine racemase, N-terminal domain (PF01168; HMM-score: 72)
    and 1 more
    no clan defined CPV_Polyhedrin; Cypovirus polyhedrin protein (PF17515; HMM-score: 13.3)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002213
    • TAT(Tat/SPI): 0.000125
    • LIPO(Sec/SPII): 0.000462
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MAHVNINISKIKYNAKVLQTVFQSKNMQFTPVIKCIAGDRTIVESLKALGINHVAESRLDNIISIADQDLTYTLLRTPAKKEISDMIEKVDMSIQTELSTIHQINEVAEVLGKKHKILLMVDWKDGREGVLTYDVLDYIKEIIHLKNIHFVGLAFNFMCFKSDAPSDDDIFMINRFVSAVEREIGYRLKIISGGNSSMLPQLLYNDLGKINELRIGETLFRGVDTTTNQAIAMLYQDAITLEAEILEIKPRVNTQTHESFLQAIVDIGYLDTKVDNISPMDQHINILGASSDHLMLDLNGQGHYQVGDHISFSLNYEALSHSMYMKNLHKVYIDDSKIDTLLQNFDVKSPAMVNHY

Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]