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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS13185 [old locus tag: NWMN_2292 ]
  • pan locus tag?: SAUPAN005923000
  • symbol: NWMN_RS13185
  • pan gene symbol?: nreB
  • synonym:
  • product: sensor histidine kinase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_RS13185 [old locus tag: NWMN_2292 ]
  • symbol: NWMN_RS13185
  • product: sensor histidine kinase
  • replicon: chromosome
  • strand: -
  • coordinates: 2517406..2518440
  • length: 1035
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_009641 (2517406..2518440) NCBI
  • BioCyc:
  • MicrobesOnline: see NWMN_2292

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGATTAATGAGGACAGTATACAGTTAGATACTTTATTGAAAAAATATTATGAGCATTCA
    ATCGAAAAGATAGTTTTTGCTGATGATAACGGTAAAATTATTGCAATGAATGATGCTGCT
    AAAGATATATTATCTGAAGAAGATAATTACAGTGCTGTGGCGAATGCGATTTGTCACAGA
    TGTGAAGGGTATACAAATGCCTATGATGTACAATCATGTAAAGACTGTTTCTTAGAATCG
    ATGCAAGTACAAGCTACGAATTTCCAAGTATTTATGAAGACGAAAGATCAAAAAGTGATG
    CCATTTACAGCAACATATCAGTTGATTGATCAAGACAGGGGAATTCATGCATTTACGCTT
    CAAAATGTTTCATCACAAATTGAACAGCAAGAGAAATTGCATCAACAACGTATGATGCGT
    AAAACAATCTCAGCCCAAGAAAATGAAAGAAAGCGAATTTCTAGAGAGTTACATGATAGT
    GTGATTCAAGAAATGCTCAATGTAGATGTTCAATTGAGATTATTAAAATATCAAGAAGAT
    ACAACTAAGCTTTTGGAAGACGCAGAAAATATTGAATATATTGTCGCTAAATTAATTGAT
    GATATACGTAATATGTCGGTTGAATTAAGACCCGCTTCTTTGGATGATCTAGGCCTTGAA
    GCAGCTTTTAAATCATATTTTAAACAGTTTGAAGAAAATTATGGAATAAAAATCATATAT
    ACATCAAATATTAAAAATACTCGCTTTGATAGTGATATTGAAACGGTTGTTTATCGAGTA
    GTGCAAGAAGCTATTTTAAATGCGTTGAAATACGCTGATGTAAATGAAATAAATGTAGGT
    ATCCGTCAAACTGGGCGCCATTTGGTAGCAGAAGTGATAGATGCGGGAAATGGTTTTGAT
    CCAAGTTCTAAGCCTAAAGGTTCTGGACTCGGTTTATATGGAATGAATGAGCGAGCTGAG
    TTAGTCAGCGGTAGCGTCAATATTGAAACGAAAATTGGTGAGGGTACCAATGTTACATTG
    AACATTCCAATTTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1035

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_RS13185 [old locus tag: NWMN_2292 ]
  • symbol: NWMN_RS13185
  • description: sensor histidine kinase
  • length: 344
  • theoretical pI: 4.65774
  • theoretical MW: 39120
  • GRAVY: -0.409302

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.13.3?  ExPASy
    Histidine kinase ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
  • TIGRFAM:
    Genetic information processing Protein fate Protein and peptide secretion and trafficking putative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 21.2)
    Signal transduction Signal transduction Two-component systems putative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 21.2)
    Signal transduction Regulatory functions Small molecule interactions PAS domain S-box protein (TIGR00229; HMM-score: 17.2)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    His_Kinase_A (CL0025) HisKA_3; Histidine kinase (PF07730; HMM-score: 53.4)
    and 5 more
    HATPase_c; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF02518; HMM-score: 38.7)
    HATPase_c_2; Histidine kinase-like ATPase domain (PF13581; HMM-score: 23.6)
    PAS_Fold (CL0183) PAS_9; PAS domain (PF13426; HMM-score: 22)
    UBA (CL0214) WIYLD; Ubiquitin-binding WIYLD domain (PF10440; HMM-score: 13.6)
    no clan defined DUF4974; Domain of unknown function (DUF4974) (PF16344; HMM-score: 12.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: [4Fe-4S] cluster
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 1.05
    • Cytoplasmic Membrane Score: 8.78
    • Cellwall Score: 0.08
    • Extracellular Score: 0.09
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.007402
    • TAT(Tat/SPI): 0.000636
    • LIPO(Sec/SPII): 0.000999
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MINEDSIQLDTLLKKYYEHSIEKIVFADDNGKIIAMNDAAKDILSEEDNYSAVANAICHRCEGYTNAYDVQSCKDCFLESMQVQATNFQVFMKTKDQKVMPFTATYQLIDQDRGIHAFTLQNVSSQIEQQEKLHQQRMMRKTISAQENERKRISRELHDSVIQEMLNVDVQLRLLKYQEDTTKLLEDAENIEYIVAKLIDDIRNMSVELRPASLDDLGLEAAFKSYFKQFEENYGIKIIYTSNIKNTRFDSDIETVVYRVVQEAILNALKYADVNEINVGIRQTGRHLVAEVIDAGNGFDPSSKPKGSGLGLYGMNERAELVSGSVNIETKIGEGTNVTLNIPI

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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