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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS14325 [old locus tag: NWMN_2496 ]
  • pan locus tag?: SAUPAN006293000
  • symbol: NWMN_RS14325
  • pan gene symbol?: panB
  • synonym:
  • product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_RS14325 [old locus tag: NWMN_2496 ]
  • symbol: NWMN_RS14325
  • product: 3-methyl-2-oxobutanoate hydroxymethyltransferase
  • replicon: chromosome
  • strand: -
  • coordinates: 2741011..2741829
  • length: 819
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_009641 (2741011..2741829) NCBI
  • BioCyc:
  • MicrobesOnline: see NWMN_2496

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    TTGAAAACAGTAAGTCAATTAATAGATATGAAACAAAAGCAAACTAAAATTTCTATGGTA
    ACAGCTTATGATTTTCCAAGTGCTAAACAAGTGGAAGCAGCGGGTATTGATATGATTCTT
    GTTGGGGATTCACTTGGTATGACTGTATTAGGTTATGAAAGTATCGTACAAGTGACGTTA
    GCAGATATGATTCATCACGGTCGTGCAGTAAGAAGAGGCGCACCAAATACATTTGTAGTT
    GTTGATATGCCGATTGGTGCAGTAGGCATCTCTATGACACAAGACTTAAATCATGCTCTA
    AAGCTTTATCAGGAGACGAATGCAAATGCAATTAAAGCAGAAGGAGCGCATATTACACCA
    TTTATTGAAAAAGCAACGGCAATTGGTATTCCTGTTGTTGCACATTTAGGATTAACACCT
    CAAAGTGTTGGCGTTATGGGGTATAAATTACAAGGTGCAACGAAAGAAGCAGCGGAGCAA
    CTTATTTTAGACGCGAAAAATGTAGAACAAGCTGGTGCAGTTGCGTTAGTACTTGAAGCA
    ATTCCAAATGATTTAGCAGAAGAAATTAGCAAGCACTTAACAATTCCAGTCATTGGTATT
    GGTGCAGGAAAAGGTACAGATGGTCAAGTATTGGTTTATCACGATATGTTAAATTATGGT
    GTTGAACATAAAGCGAAATTTGTGAAGCAATTTGCTGATTTTAGTGTTGGTGTGGATGGT
    TTAAAACAATACGATCAAGAAGTTAAATCTGGTGCATTTCCATCAGAAGAATATACGTAT
    AAAAAGAAAATTATGAATGAGGTTAATAACAATGACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    819

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_RS14325 [old locus tag: NWMN_2496 ]
  • symbol: NWMN_RS14325
  • description: 3-methyl-2-oxobutanoate hydroxymethyltransferase
  • length: 272
  • theoretical pI: 5.84619
  • theoretical MW: 29267.5
  • GRAVY: 0.00294118

⊟Function[edit | edit source]

  • reaction:
    EC 2.1.2.11?  ExPASy
    3-methyl-2-oxobutanoate hydroxymethyltransferase 5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate + H2O = tetrahydrofolate + 2-dehydropantoate
  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pantothenate and coenzyme A 3-methyl-2-oxobutanoate hydroxymethyltransferase (TIGR00222; EC 2.1.2.11; HMM-score: 294.2)
    and 1 more
    Metabolism Energy metabolism Amino acids and amines aspartate racemase (TIGR00035; EC 5.1.1.13; HMM-score: 13.7)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    PK_TIM (CL0151) Pantoate_transf; Ketopantoate hydroxymethyltransferase (PF02548; HMM-score: 320.3)
    and 3 more
    PEP_mutase; Phosphoenolpyruvate phosphomutase (PF13714; HMM-score: 19.2)
    TIM_barrel (CL0036) NanE; Putative N-acetylmannosamine-6-phosphate epimerase (PF04131; HMM-score: 15.5)
    Leu-IlvD (CL0364) PA; PA domain (PF02225; HMM-score: 13.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Mg2+
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.019194
    • TAT(Tat/SPI): 0.000656
    • LIPO(Sec/SPII): 0.000597
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKTVSQLIDMKQKQTKISMVTAYDFPSAKQVEAAGIDMILVGDSLGMTVLGYESIVQVTLADMIHHGRAVRRGAPNTFVVVDMPIGAVGISMTQDLNHALKLYQETNANAIKAEGAHITPFIEKATAIGIPVVAHLGLTPQSVGVMGYKLQGATKEAAEQLILDAKNVEQAGAVALVLEAIPNDLAEEISKHLTIPVIGIGAGKGTDGQVLVYHDMLNYGVEHKAKFVKQFADFSVGVDGLKQYDQEVKSGAFPSEEYTYKKKIMNEVNNND

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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