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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus Newman
- locus tag: NWMN_RS14950 [old locus tag: NWMN_2611 ]
- pan locus tag?: SAUPAN006488000
- symbol: NWMN_RS14950
- pan gene symbol?: gidA
- synonym:
- product: tRNA uridine-5-carboxymethylaminomethyl(34) synthesis enzyme MnmG
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: NWMN_RS14950 [old locus tag: NWMN_2611 ]
- symbol: NWMN_RS14950
- product: tRNA uridine-5-carboxymethylaminomethyl(34) synthesis enzyme MnmG
- replicon: chromosome
- strand: -
- coordinates: 2874610..2876487
- length: 1878
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1861GTGGTTCAAGAATATGATGTAATCGTTATAGGTGCGGGACATGCAGGTGTAGAAGCAGGT
TTAGCATCTGCAAGACGTGGTGCTAAAACATTAATGCTAACAATAAATTTAGATAATATT
GCATTTATGCCATGTAACCCATCTGTAGGTGGACCAGCTAAAGGTATCGTTGTTCGTGAA
ATTGATGCTTTAGGTGGACAAATGGCAAAAACAATCGATAAAACACACATTCAAATGAGA
ATGTTAAATACAGGTAAAGGACCTGCTGTAAGAGCACTAAGAGCGCAAGCAGATAAAGTA
CTTTATCAACAAGAAATGAAACGCGTGATTGAAGATGAAGAAAATTTGCATATAATGCAA
GGTATGGTAGACGAACTTATTATAGAAGATAATGAAGTTAAAGGTGTACGTACAAATATT
GGTACAGAGTATTTATCTAAAGCAGTAATTATTACAACGGGAACATTTTTACGTGGTGAA
ATCATTTTAGGTAATATGAAGTATTCAAGTGGACCAAATCACCAATTACCATCAATCACA
TTATCAGACAATTTAAGAGAACTTGGTTTTGATATTGTTCGTTTTAAAACAGGTACACCA
CCGCGTGTAAATTCAAAAACAATTGACTATTCGAAGACTGAAATACAACCAGGTGACGAT
GTAGGTCGTGCATTCAGCTTTGAAACAACAGAATATATATTAGATCAATTGCCATGTTGG
CTAACGTATACTAATGCTGAAACACACAAAGTTATCGATGATAATTTACATCTATCTGCA
ATGTATTCAGGGATGATTAAAGGAACCGGGCCACGTTATTGCCCTTCAATTGAAGATAAA
TTTGTTCGATTTAATGATAAGCCGCGACATCAACTTTTCTTAGAGCCTGAAGGTCGTAAT
ACAAATGAAGTATATGTGCAAGGATTGTCTACAAGTCTTCCTGAACATGTGCAACGTCAA
ATGTTAGAGACGATACCAGGTCTTGAAAAAGCAGATATGATGCGTGCCGGCTACGCAATT
GAATATGATGCGATTGTGCCAACGCAGTTATGGCCTACACTTGAAACGAAAATGATTAAA
AACTTATATACTGCAGGTCAAATTAATGGTACATCTGGTTATGAAGAAGCAGCAGGACAA
GGATTGATGGCAGGTATTAACGCTGCAGGTAAAGTGTTAAACACAGGCGAAAAGATATTA
AGTCGTTCAGATGCATATATTGGTGTCTTAATCGATGATCTTGTAACTAAAGGTACTAAT
GAACCTTATCGTTTACTAACATCACGTGCAGAATATCGTTTGTTACTACGTCATGATAAT
GCTGATTTGAGATTGACGGATATGGGATATGAACTTGGTATGATTTCTGAAGAAAGATAT
GCACGTTTTAATGAAAAACGTCAGCAAATTGATGCGGAAATTAAGCGTTTATCAGATATT
CGTATTAAACCAAACGAACATACGCAAGCGATTATTGAACAACATGGTGGTTCTCGCTTA
AAAGATGGTATTTTAGCTATCGATTTATTACGCAGACCTGAAATGACTTACGATATAATT
TTAGAACTTTTAGAAGAAGAACATCAATTGAATGCAGATGTTGAAGAACAAGTAGAAATA
CAAACAAAATATGAAGGTTATATCAATAAATCACTACAACAAGTTGAGAAAGTTAAGCGT
ATGGAAGAGAAGAAAATTCCAGAAGACTTAGATTATAGTAAGATTGATAGTTTGGCGACT
GAAGCGCGAGAAAAATTATCAGAAGTAAAACCTTTAAATATTGCACAAGCTTCTAGAATA
TCAGGGGTAAATCCAGCAGACATATCTATATTATTGATTTACTTAGAACAAGGTAAACTC
CAAAGGGTGAGTGACTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: NWMN_RS14950 [old locus tag: NWMN_2611 ]
- symbol: NWMN_RS14950
- description: tRNA uridine-5-carboxymethylaminomethyl(34) synthesis enzyme MnmG
- length: 625
- theoretical pI: 5.14884
- theoretical MW: 70115.6
- GRAVY: -0.3928
⊟Function[edit | edit source]
- TIGRFAM: Protein synthesis tRNA and rRNA base modification tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA (TIGR00136; HMM-score: 955.7)and 20 moreProtein synthesis tRNA and rRNA base modification tRNA:m(5)U-54 methyltransferase (TIGR00137; EC 2.1.1.74; HMM-score: 59.8)Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll geranylgeranyl reductase family (TIGR02032; EC 1.3.1.-; HMM-score: 23.6)Energy metabolism Electron transport thioredoxin-disulfide reductase (TIGR01292; EC 1.8.1.9; HMM-score: 23.3)Cellular processes Detoxification alkyl hydroperoxide reductase subunit F (TIGR03140; EC 1.8.1.-; HMM-score: 22.9)Cellular processes Adaptations to atypical conditions alkyl hydroperoxide reductase subunit F (TIGR03140; EC 1.8.1.-; HMM-score: 22.9)Energy metabolism Anaerobic glycerol-3-phosphate dehydrogenase, anaerobic, B subunit (TIGR03378; EC 1.1.5.3; HMM-score: 20.9)putative alkyl hydroperoxide reductase F subunit (TIGR03143; EC 1.6.4.-; HMM-score: 19.6)Energy metabolism Amino acids and amines sarcosine oxidase, monomeric form (TIGR01377; HMM-score: 16.2)Energy metabolism Electron transport glutathione-disulfide reductase (TIGR01424; EC 1.8.1.7; HMM-score: 15.4)Energy metabolism TCA cycle succinate dehydrogenase, flavoprotein subunit (TIGR01816; HMM-score: 15.3)Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll geranylgeranyl reductase (TIGR02023; EC 1.3.1.-; HMM-score: 14.7)Energy metabolism Amino acids and amines sarcosine oxidase, alpha subunit family (TIGR01372; HMM-score: 13.6)dihydrolipoyl dehydrogenase (TIGR01350; EC 1.8.1.4; HMM-score: 13.4)Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine thiazole biosynthesis enzyme (TIGR00292; HMM-score: 12.1)Energy metabolism Electron transport flavocytochrome c (TIGR01813; HMM-score: 11.2)trypanothione-disulfide reductase (TIGR01423; EC 1.8.1.12; HMM-score: 10.7)Unknown function Enzymes of unknown specificity putative bacillithiol system oxidoreductase, YpdA family (TIGR04018; EC 1.8.-.-; HMM-score: 10.6)Biosynthesis of cofactors, prosthetic groups, and carriers Pyridine nucleotides L-aspartate oxidase (TIGR00551; EC 1.4.3.16; HMM-score: 9.7)Cellular processes Detoxification mercury(II) reductase (TIGR02053; EC 1.16.1.1; HMM-score: 8.1)Unknown function Enzymes of unknown specificity flavoprotein, HI0933 family (TIGR00275; HMM-score: 7.9)
- TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
- PFAM: NADP_Rossmann (CL0063) GIDA; Glucose inhibited division protein A (PF01134; HMM-score: 563.2)and 7 moreno clan defined GIDA_assoc; GidA associated domain (PF13932; HMM-score: 267.7)NADP_Rossmann (CL0063) FAD_binding_2; FAD binding domain (PF00890; HMM-score: 22.7)Pyr_redox_2; Pyridine nucleotide-disulphide oxidoreductase (PF07992; HMM-score: 22.4)FAD_oxidored; FAD dependent oxidoreductase (PF12831; HMM-score: 20.2)Pyr_redox_3; Pyridine nucleotide-disulphide oxidoreductase (PF13738; HMM-score: 15.1)Thi4; Thi4 family (PF01946; HMM-score: 11.8)HI0933_like; HI0933-like protein (PF03486; HMM-score: 10.8)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors: FAD
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.020853
- TAT(Tat/SPI): 0.007512
- LIPO(Sec/SPII): 0.007355
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MVQEYDVIVIGAGHAGVEAGLASARRGAKTLMLTINLDNIAFMPCNPSVGGPAKGIVVREIDALGGQMAKTIDKTHIQMRMLNTGKGPAVRALRAQADKVLYQQEMKRVIEDEENLHIMQGMVDELIIEDNEVKGVRTNIGTEYLSKAVIITTGTFLRGEIILGNMKYSSGPNHQLPSITLSDNLRELGFDIVRFKTGTPPRVNSKTIDYSKTEIQPGDDVGRAFSFETTEYILDQLPCWLTYTNAETHKVIDDNLHLSAMYSGMIKGTGPRYCPSIEDKFVRFNDKPRHQLFLEPEGRNTNEVYVQGLSTSLPEHVQRQMLETIPGLEKADMMRAGYAIEYDAIVPTQLWPTLETKMIKNLYTAGQINGTSGYEEAAGQGLMAGINAAGKVLNTGEKILSRSDAYIGVLIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLRLTDMGYELGMISEERYARFNEKRQQIDAEIKRLSDIRIKPNEHTQAIIEQHGGSRLKDGILAIDLLRRPEMTYDIILELLEEEHQLNADVEEQVEIQTKYEGYINKSLQQVEKVKRMEEKKIPEDLDYSKIDSLATEAREKLSEVKPLNIAQASRISGVNPADISILLIYLEQGKLQRVSD
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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