PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA0035
• pan locus tag^?: SAUPAN000087000
• symbol: SA0035
• pan gene symbol^?: —
• synonym:
• product: HMG-CoA synthase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA0035
• symbol: SA0035
• product: HMG-CoA synthase
• replicon: chromosome
• strand: +
• coordinates: 42539..42880
• length: 342
• essential: no DEG

⊟Accession numbers

• Gene ID: 1122809 NCBI
• RefSeq: NP_373275 NCBI
• BioCyc:
• MicrobesOnline: 102301 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  
  ATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAA
  CCAGAAGAGAAAGTATGTCTATTTAGTTATGGTTCAGGAGCAGTAGGAGAAATCTTTAGT
  GGTTCAATCGTTAAAGGATATGACAAAGCATTAGATAAAGAGAAACACTTAAATATGCTA
  GAATCTAGAGAGCAATTATCAGTCGAAGAATACGAAACATTCTTTAACAGATTTGATAAT
  CAAGAATTTGATTTCGAACGTGAATTGACACAAGATCCATATTCAAAAGTATACTTATAC
  AGTATAGAAGACCATATCAGAACATATAAGATAGAGAAATAA
  60
  120
  180
  240
  300
  342
  

⊟Protein

⊟General

• locus tag: SA0035
• symbol: SA0035
• description: HMG-CoA synthase
• length: 113
• theoretical pI: 4.53054
• theoretical MW: 13360.8
• GRAVY: -0.566372

⊟Function

• TIGRFAM:
  hydroxymethylglutaryl-CoA synthase (TIGR01835; EC 2.3.3.10; HMM-score: 97.8)
  and 2 more
  hydroxymethylglutaryl-CoA synthase (TIGR01833; EC 2.3.3.10; HMM-score: 35.4)
  hopanoid biosynthesis associated membrane protein HpnM (TIGR03481; HMM-score: 11.6)
• TheSEED  :
  • Hydroxymethylglutaryl-CoA synthase N-domain homolog (EC 2.3.3.10)
• PFAM:
  Thiolase (CL0046) HMG_CoA_synt_C; Hydroxymethylglutaryl-coenzyme A synthase C terminal (PF08540; HMM-score: 39.7)
  and 2 more
  GT-A (CL0110) CgtA; Beta-1,4-N-acetylgalactosaminyltransferase (CgtA) (PF06306; HMM-score: 14.3)
  tRNA_synt_II (CL0040) tRNA-synt_2e; Glycyl-tRNA synthetase alpha subunit (PF02091; HMM-score: 13.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.041852
  • TAT(Tat/SPI): 0.001039
  • LIPO(Sec/SPII): 0.004545
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15925742 NCBI
• RefSeq: NP_373275 NCBI
• UniProt: A0A0H3JK22 UniProt

⊟Protein sequence

• MYTGSLYLSLISLLQNHTFQPEEKVCLFSYGSGAVGEIFSGSIVKGYDKALDKEKHLNMLESREQLSVEEYETFFNRFDNQEFDFERELTQDPYSKVYLYSIEDHIRTYKIEK

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications