PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA0048 [new locus tag: SA_RS00385 ]
• pan locus tag^?: SAUPAN000063000
• symbol: ermA
• pan gene symbol^?: ermA1
• synonym:
• product: rRNA methylase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA0048 [new locus tag: SA_RS00385 ]
• symbol: ermA
• product: rRNA methylase
• replicon: chromosome
• strand: +
• coordinates: 56002..56733
• length: 732
• essential: no DEG

⊟Accession numbers

• Gene ID: 1122822 NCBI
• RefSeq: NP_373288 NCBI
• BioCyc: see SA_RS00385
• MicrobesOnline: 102314 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGAACCAGAAAAACCCTAAAGACACGCAAAATTTTATTACTTCTAAAAAGCATGTAAAA
  GAAATATTGAATCACACGAATATCAGTAAACAAGACAACGTAATAGAAATCGGATCAGGA
  AAAGGACATTTTACCAAAGAGCTAGTCAAAATGAGTCGATCAGTTACTGCTATAGAAATT
  GATGGAGGCTTATGTCAAGTGACTAAAGAAGCGGTAAACCCCTCTGAGAATATAAAAGTG
  ATTCAAACGGATATTCTAAAATTTTCCTTCCCAAAACATATAAACTATAAGATATATGGT
  AATATTCCTTATAACATCAGTACGGATATTGTCAAAAGAATTACCTTTGAAAGTCAGGCT
  AAATATAGCTATCTTATCGTTGAGAAGGGATTTGCGAAAAGATTGCAAAATCTGCAACGA
  GCTTTGGGTTTACTATTAATGGTGGAGATGGATATAAAAATGCTCAAAAAAGTACCACCA
  CTATATTTTCATCCTAAGCCAAGTGTAGACTCTGTATTGATTGTTCTTGAACGACATCAA
  CCATTGATTTCAAAGAAGGACTACAAAAAGTATCGATCTTTTGTTTATAAGTGGGTAAAC
  CGTGAATATCGTGTTCTTTTCACTAAAAACCAATTCCGACAGGCTTTGAAGCATGCAAAT
  GTCACTAATATTAATAAACTATCGAAGGAACAATTTCTTTCTATTTTCAATAGTTACAAA
  TTGTTTCACTAA
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⊟Protein

⊟General

• locus tag: SA0048 [new locus tag: SA_RS00385 ]
• symbol: ErmA
• description: rRNA methylase
• length: 243
• theoretical pI: 10.5175
• theoretical MW: 28408
• GRAVY: -0.430453

⊟Function

• reaction:
  EC 2.1.1.184?  ExPASy
  23S rRNA (adenine²⁰⁸⁵-N⁶)-dimethyltransferase 2 S-adenosyl-L-methionine + adenine²⁰⁸⁵ in 23S rRNA = 2 S-adenosyl-L-homocysteine + N⁶-dimethyladenine²⁰⁸⁵ in 23S rRNA
• TIGRFAM:
  Genetic information processing Protein synthesis tRNA and rRNA base modification ribosomal RNA small subunit methyltransferase A (TIGR00755; EC 2.1.1.182; HMM-score: 141.2)
  and 9 more
  Unknown function Enzymes of unknown specificity putative methylase (TIGR00537; HMM-score: 25)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Biotin malonyl-acyl carrier protein O-methyltransferase BioC (TIGR02072; EC 2.1.1.-; HMM-score: 24.4)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin precorrin-6Y C5,15-methyltransferase (decarboxylating), CbiT subunit (TIGR02469; EC 2.1.1.132; HMM-score: 20.9)
  Genetic information processing Protein fate Protein modification and repair protein-L-isoaspartate O-methyltransferase (TIGR00080; EC 2.1.1.77; HMM-score: 15.8)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll magnesium protoporphyrin O-methyltransferase (TIGR02021; EC 2.1.1.11; HMM-score: 15.1)
  Genetic information processing Protein synthesis tRNA and rRNA base modification 23S rRNA (uracil-5-)-methyltransferase RumA (TIGR00479; EC 2.1.1.-; HMM-score: 14.6)
  Metabolism Transport and binding proteins Anions phosphate ABC transporter, ATP-binding protein (TIGR00972; EC 3.6.3.27; HMM-score: 13.9)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone ubiquinone/menaquinone biosynthesis methyltransferase (TIGR01934; EC 2.1.1.-; HMM-score: 13.5)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone 3-demethylubiquinone-9 3-O-methyltransferase (TIGR01983; EC 2.1.1.64; HMM-score: 12.9)
• TheSEED  :
  • 23S rRNA (adenine(2058)-N(6))-dimethyltransferase (EC 2.1.1.184) => Erm(A)
• PFAM:
  NADP_Rossmann (CL0063) RrnaAD; Ribosomal RNA adenine dimethylase (PF00398; HMM-score: 253.3)
  and 11 more
  Methyltransf_25; Methyltransferase domain (PF13649; HMM-score: 27.2)
  NodS; Nodulation protein S (NodS) (PF05401; HMM-score: 24.9)
  Methyltransf_11; Methyltransferase domain (PF08241; HMM-score: 23.6)
  PCMT; Protein-L-isoaspartate(D-aspartate) O-methyltransferase (PCMT) (PF01135; HMM-score: 17.3)
  Methyltransf_31; Methyltransferase domain (PF13847; HMM-score: 17)
  Methyltransf_32; Methyltransferase domain (PF13679; HMM-score: 15.8)
  FtsJ; FtsJ-like methyltransferase (PF01728; HMM-score: 15.1)
  DOT1; Histone methylation protein DOT1 (PF08123; HMM-score: 14.9)
  Methyltransf_23; Methyltransferase domain (PF13489; HMM-score: 14.8)
  Methyltransf_4; Putative methyltransferase (PF02390; HMM-score: 13.5)
  LYR-like (CL0491) Complex1_LYR_2; Complex1_LYR-like (PF13233; HMM-score: 11.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.67
  • Cytoplasmic Membrane Score: 0.01
  • Cellwall Score: 0.15
  • Extracellular Score: 0.17
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.020332
  • TAT(Tat/SPI): 0.00071
  • LIPO(Sec/SPII): 0.000703
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15925755 NCBI
• RefSeq: NP_373288 NCBI
• UniProt: P0A0H2 UniProt

⊟Protein sequence

• MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHFTKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNIPYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPPLYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKHANVTNINKLSKEQFLSIFNSYKLFH

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

T Ito, Y Katayama, K Hiramatsu
Cloning and nucleotide sequence determination of the entire mec DNA of pre-methicillin-resistant Staphylococcus aureus N315.
Antimicrob Agents Chemother: 1999, 43(6);1449-58
[PubMed:10348769] [WorldCat.org] [DOI] (P p)