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NCBI: 26-AUG-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus N315
- locus tag: SA0052 [new locus tag: SA_RS00405 ]
- pan locus tag?: SAUPAN000138000
- symbol: tnpA
- pan gene symbol?: tnpA
- synonym:
- product: transposase A for Tn554
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SA0052 [new locus tag: SA_RS00405 ]
- symbol: tnpA
- product: transposase A for Tn554
- replicon: chromosome
- strand: -
- coordinates: 60065..61150
- length: 1086
- essential: no DEG
⊟Accession numbers[edit | edit source]
- Gene ID: 1122826 NCBI
- RefSeq: NP_373292 NCBI
- BioCyc: see SA_RS00405
- MicrobesOnline: 102318 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1081GTGAAGGTTCAAAGGATAGAAGTGGAGAATAAGCCGTATCCATTGTATTTATTACTAGAT
AAAGAATACCAGCTAATAGAACCAGTAATGAAATTTATTAAATACTTAGATAATACTGGT
AAGTCTCCTAATACCATTAAGGCATACTGCTATCATTTAAAGTTGCTGTACGAGTTCATG
GAACAGAGAGGTGTTATTCTTAATGATATTAACTTTGAGTTGTTAGCAGACTTCGTAGGT
TGGTTGAGATATCCTTCAGCATCAAATGTAATTGATCTTCAGTCAAAAAAAGCCATAAGA
GAAGAAACGACAGTGAATACAATTTTAAATGTAGTTATGAGTTTTCTTGATTATTTAAGT
AGATTAGGAGAATTTAAATCAATTGATGTATTTAAACAAGCAAAGGGAAGAAATTTCAAA
GGATTTTTACATCATGTTAATAAGGGTAGATACCAAAAGAATGTCTTAAAGTTAAGGGTT
AAAAAGAAACAGATAAGAACATTGAGATCAAAGGAAGTTAAGCAAATTATTGATGCTTGT
CATACGAAAAGAGATAAATTAATTTTAATGCTTATGTATGAGGGTGGTTTAAGAATCGGT
GAAGTGTTATCGCTTAGGCTTGAAGATATTGTCACTTGGGACAATCAAATCCATTTAACA
CCTAGAGATGTTAATGTTAATGAAGCTTATATTAAATTAAGGAAGGAAAGAACAATACAT
GTGAGTAAAGAACTTATGTCACTTTATACAGATTACTTGATATATGAGTATAGTGAGGAA
TTGGAGCATGATTATGTTTTTATTTCCTTAAAAGAAGGCTATTTTGGGAAACCACTAAAG
TACCAAAGTGTTCTTGATCTAGTTAGAAGAATAGTTAAAAGGACTGGAATAGAATTTACA
TCACATATGCTTCGCCACACTCACGCAACGCAGCTAATTAGGGAAGGATGGGATGTTGCG
TTCGTTCAAAAGAGATTAGGTCACGCACATGTTCAGACAACGTTAAATACCTATGTTCAT
CTTTCAGATCAGGATATGAAAAATGAGTTTAATAAATACCTCGAGAGAAAGGAGCATAAG
AAATGA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SA0052 [new locus tag: SA_RS00405 ]
- symbol: TnpA
- description: transposase A for Tn554
- length: 361
- theoretical pI: 9.89064
- theoretical MW: 42940.7
- GRAVY: -0.441828
⊟Function[edit | edit source]
- TIGRFAM: DNA metabolism DNA replication, recombination, and repair tyrosine recombinase XerC (TIGR02224; HMM-score: 108.3)DNA metabolism DNA replication, recombination, and repair tyrosine recombinase XerD (TIGR02225; HMM-score: 105.8)and 2 moreDNA metabolism DNA replication, recombination, and repair integron integrase (TIGR02249; HMM-score: 58.1)Mobile and extrachromosomal element functions Other integron integrase (TIGR02249; HMM-score: 58.1)
- TheSEED :
- Transposase A from transposon Tn554
- PFAM: DNA-mend (CL0382) Phage_integrase; Phage integrase family (PF00589; HMM-score: 154.1)and 3 morel-integrase_N (CL0469) Phage_int_SAM_1; Phage integrase, N-terminal SAM-like domain (PF02899; HMM-score: 31.1)Phage_int_SAM_4; Phage integrase, N-terminal SAM-like domain (PF13495; HMM-score: 23.8)Phage_int_SAM_5; Phage integrase SAM-like domain (PF13102; HMM-score: 20.3)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 9.97
- Cytoplasmic Membrane Score: 0
- Cellwall Score: 0.01
- Extracellular Score: 0.02
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.002319
- TAT(Tat/SPI): 0.000069
- LIPO(Sec/SPII): 0.000316
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MKVQRIEVENKPYPLYLLLDKEYQLIEPVMKFIKYLDNTGKSPNTIKAYCYHLKLLYEFMEQRGVILNDINFELLADFVGWLRYPSASNVIDLQSKKAIREETTVNTILNVVMSFLDYLSRLGEFKSIDVFKQAKGRNFKGFLHHVNKGRYQKNVLKLRVKKKQIRTLRSKEVKQIIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIVTWDNQIHLTPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFGKPLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTTLNTYVHLSDQDMKNEFNKYLERKEHKK
⊟Experimental data[edit | edit source]
- experimentally validated:
- protein localization:
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: no data available
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
⊟Relevant publications[edit | edit source]
T Ito, Y Katayama, K Hiramatsu
Cloning and nucleotide sequence determination of the entire mec DNA of pre-methicillin-resistant Staphylococcus aureus N315.
Antimicrob Agents Chemother: 1999, 43(6);1449-58
[PubMed:10348769] [WorldCat.org] [DOI] (P p)