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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0524 [new locus tag: SA_RS03050 ]
  • pan locus tag?: SAUPAN002343000
  • symbol: SA0524
  • pan gene symbol?: folE2
  • synonym:
  • product: GTP cyclohydrolase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA0524 [new locus tag: SA_RS03050 ]
  • symbol: SA0524
  • product: GTP cyclohydrolase
  • replicon: chromosome
  • strand: -
  • coordinates: 619909..620787
  • length: 879
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGACTGAATTTGATTTATCGACACGAGAAGGTCGTTGGAAACACTTTGGTTCCGTAGAT
    CCTATTGAAGGCACGAAACCTACTACTAAGAACGAAATGACAGATTTACAAAGTACACAT
    AAAGATTTTTTATTTGAAATAGAAGAAGTTGGTATTAAAAACTTAGTGTACCCTGTACTT
    GTTGACCAGTATCAAACAGCTGGCACATTTAGTTTTTCAACAAGTTTAACAAAAGACGAA
    AAAGGTATTAATATGAGCCGTATAATTGAAAGTGTTGAAAAGCACTATGATAATGGTATT
    GAACTTGAATTTAATACACTTTATCAGGTGTTACGCACTTTACAAACGAATATGAAACAA
    AATGCGGCAGGCGTTGATGTTTCTGGGAAATGGTTCTTTGACCGCTATAGTCCAACCACA
    AATATTAAAGCAGTTGGTAATGCCGATGTCACTTATGGATTAGCAATTGACGGTGATAAA
    GTTACACGTAAAGAACTTACTATTGAAGCAACTGTCACAACGCTATGCCCTTGTTCAAAA
    GAAATAAGTGAGTATTCAGCGCACAACCAACGTGGTGTTGTCACTGTTAAAACATATATT
    AATAAAGATCAAAACATAGTTGATGACTATAAAAACAAAATTTTAGATGCAATGGAAGCT
    AATGCAAGTTCAATTCTATACCCTATTTTAAAACGTCCTGATGAAAAACGTGTGACAGAG
    CGTGCATATGAAAATCCACGTTTTGTCGAAGACTTAATTCGTTTAATTGCCGCGGACTTA
    GTTGAATTTGATTGGTTAGACGGTTTCGATATCGAATGTCGTAATGAAGAGTCTATCCAT
    CAACATGATGCTTTCGCTAAATTAAAGTATCGCAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    879

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA0524 [new locus tag: SA_RS03050 ]
  • symbol: SA0524
  • description: GTP cyclohydrolase
  • length: 292
  • theoretical pI: 4.93644
  • theoretical MW: 33480.4
  • GRAVY: -0.542466

⊟Function[edit | edit source]

  • reaction:
    EC 3.5.4.16?  ExPASy
    GTP cyclohydrolase I GTP + H2O = formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate
  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other GTP cyclohydrolase (TIGR00294; EC 3.5.4.-; HMM-score: 169.1)
  • TheSEED  :
    • GTP cyclohydrolase I (EC 3.5.4.16) type 2
    Cofactors, Vitamins, Prosthetic Groups, Pigments Folate and pterines Folate Biosynthesis  GTP cyclohydrolase I (EC 3.5.4.16) type 2
    and 1 more
    RNA Metabolism RNA processing and modification Queuosine-Archaeosine Biosynthesis  GTP cyclohydrolase I (EC 3.5.4.16) type 2
  • PFAM:
    THBO-biosyn (CL0334) GCHY-1; Type I GTP cyclohydrolase folE2 (PF02649; HMM-score: 235.2)
    and 1 more
    HTH (CL0123) PadR; Transcriptional regulator PadR-like family (PF03551; HMM-score: 14.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.005134
    • TAT(Tat/SPI): 0.00032
    • LIPO(Sec/SPII): 0.001326
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTEFDLSTREGRWKHFGSVDPIEGTKPTTKNEMTDLQSTHKDFLFEIEEVGIKNLVYPVLVDQYQTAGTFSFSTSLTKDEKGINMSRIIESVEKHYDNGIELEFNTLYQVLRTLQTNMKQNAAGVDVSGKWFFDRYSPTTNIKAVGNADVTYGLAIDGDKVTRKELTIEATVTTLCPCSKEISEYSAHNQRGVVTVKTYINKDQNIVDDYKNKILDAMEANASSILYPILKRPDEKRVTERAYENPRFVEDLIRLIAADLVEFDWLDGFDIECRNEESIHQHDAFAKLKYRK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Alexander Scherl, Patrice François, Manuela Bento, Jacques M Deshusses, Yvan Charbonnier, Véronique Converset, Antoine Huyghe, Nadia Walter, Christine Hoogland, Ron D Appel, Jean-Charles Sanchez, Catherine G Zimmermann-Ivol, Garry L Corthals, Denis F Hochstrasser, Jacques Schrenzel
Correlation of proteomic and transcriptomic profiles of Staphylococcus aureus during the post-exponential phase of growth.
J Microbiol Methods: 2005, 60(2);247-57
[PubMed:15590099] [WorldCat.org] [DOI] (P p)

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