PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA0541 [new locus tag: SA_RS03135 ]
• pan locus tag^?: SAUPAN002362000
• symbol: SA0541
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA0541 [new locus tag: SA_RS03135 ]
• symbol: SA0541
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 634017..635501
• length: 1485
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1123347 NCBI
• RefSeq: NP_373795 NCBI
• BioCyc: see SA_RS03135
• MicrobesOnline: 102821 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  1441
  
  ATGGAAAAAAAGAATAAGCAAATAGATAGAGGCGATTTAAAACAAAACCTATCTGAAAAG
  TTTGTATGGGCGATTGCATATGGTTCATGTATCGGATGGGGCGCATTCATCTTACCAGGA
  GACTGGATTAAGCAGTCAGGTCCGATTGCAGCATCAATTGGTATAGTTATTGGTGCATTA
  TTAATGATATTAATTGCGGTTAGTTATGGCGCATTAGTAGAGAGATTTCCAGTATCAGGG
  GGCGCGTTTGCCTTTAGTTTCTTAAGTTTCGGCAGATATGTGAGTTTCTTCTCATCATGG
  TTTTTAACTTTTGGTTATGTCTGTGTCGTTGCTTTAAATGCGACCGCATTCAGTTTACTA
  GTTAAATTCTTATTGCCAGATGTCTTAAATAATGGGAAACTATACACCATTGCGGGCTGG
  GACGTTTATATTACGGAAATCATTATTGCGACCGTATTACTACTTGTATTCATGCTAGTA
  ACGATTCGTGGCGCAAGTGTATCTGGATCATTACAATATTATTTCTGTGTGGCGATGGTA
  ATCGTCGTATTATTGATGTTCTTTGGTTCATTCTTTGGTAATAATTTTGCACTTGAAAAT
  TTACAACCGTTAGCTGAACCTAGCAAAGGATGGTTAGTGTCTATTGTGGTTATTGTATCC
  GTGGCACCATGGGCATATGTTGGATTTGATAATATTCCACAAACAGCAGAAGAGTTTAAC
  TTTGCACCAAACAAGACATTTAAGCTTATCGTGTACAGTTTATTAGCAGCATCATTAACT
  TATGTTGTCATGATTTTATACACTGGTTGGTTATCAACAAGTCATCAAAGTTTAAATGGG
  CAGTTGTGGTTAACAGGTGCTGTTACACAAACAGCATTTGGTTATATTGGATTAGGTGTA
  TTAGCAATTGCAATTATGATGGGTATATTTACTGGTTTAAATGGATTCTTGATGAGTTCA
  AGTCGCTTGTTATTTTCTATGGGACGTTCAGGTATTATGCCAACAATGTTTAGTAAATTA
  CATAGTAAATACAAAACACCATATGTCGCAATCATATTCCTAGTAGGTGTGTCGTTAATT
  GCACCTTGGCTAGGAAGAACTGCATTGACTTGGATTGTAGATATGTCATCTACAGGTGTA
  TCCATTGCCTACTTTATTACATGTTTGTCTGCAGCGAAATTATTCAGTTATAACAAACAA
  AGTAATACGTATGCACCGGTTTACAAAACGTTTGCTATTATCGGCTCATTTGTATCATTC
  ATTTTCTTAGCGTTGTTATTAGTGCCAGGTTCTCCTGCAGCACTGACTGCACCATCTTAT
  ATTGCATTGCTTGGATGGTTAATCATCGGTTTAATATTCTTTGTGATTCGATATCCTAAA
  TTGAAAAATATGGATAATGATGAATTAAGTCGCTTGATTTTAAATAGAAGTGAAAATGAA
  GTTGATGATATGATTGAAGAACCTGAAAAAGAAAAAACTAAATAA
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⊟Protein

⊟General

• locus tag: SA0541 [new locus tag: SA_RS03135 ]
• symbol: SA0541
• description: hypothetical protein
• length: 494
• theoretical pI: 9.05617
• theoretical MW: 54336.8
• GRAVY: 0.731579

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Amino acids, peptides and amines amino acid transporter (TIGR00909; HMM-score: 129)
  and 8 more
  Metabolism Transport and binding proteins Amino acids, peptides and amines cationic amino acid transport permease (TIGR00906; HMM-score: 75.3)
  Metabolism Transport and binding proteins Amino acids, peptides and amines transporter, basic amino acid/polyamine antiporter (APA) family (TIGR00905; HMM-score: 65.8)
  Metabolism Transport and binding proteins Amino acids, peptides and amines L-type amino acid transporter (TIGR00911; HMM-score: 56.8)
  Metabolism Transport and binding proteins Amino acids, peptides and amines amino acid permease (yeast) (TIGR00913; HMM-score: 48.7)
  permease, urea carboxylase system (TIGR03428; HMM-score: 47.2)
  Metabolism Transport and binding proteins Amino acids, peptides and amines ethanolamine permease (TIGR00908; HMM-score: 35.5)
  Metabolism Transport and binding proteins Amino acids, peptides and amines arginine-ornithine antiporter (TIGR03810; HMM-score: 29.8)
  Metabolism Transport and binding proteins Amino acids, peptides and amines GABA permease (TIGR01773; HMM-score: 23.2)
• TheSEED  :
  • cationic amino acid transporter
• PFAM:
  APC (CL0062) AA_permease_2; Amino acid permease (PF13520; HMM-score: 140.4)
  and 2 more
  AA_permease; Amino acid permease (PF00324; HMM-score: 73.7)
  no clan defined DUF2615; Protein of unknown function (DUF2615) (PF11027; HMM-score: 8.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 14
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.02156
  • TAT(Tat/SPI): 0.000941
  • LIPO(Sec/SPII): 0.086079
• predicted transmembrane helices (TMHMM): 12

⊟Accession numbers

• GI: 15926262 NCBI
• RefSeq: NP_373795 NCBI
• UniProt: A0A0H3JNX6 UniProt

⊟Protein sequence

• MEKKNKQIDRGDLKQNLSEKFVWAIAYGSCIGWGAFILPGDWIKQSGPIAASIGIVIGALLMILIAVSYGALVERFPVSGGAFAFSFLSFGRYVSFFSSWFLTFGYVCVVALNATAFSLLVKFLLPDVLNNGKLYTIAGWDVYITEIIIATVLLLVFMLVTIRGASVSGSLQYYFCVAMVIVVLLMFFGSFFGNNFALENLQPLAEPSKGWLVSIVVIVSVAPWAYVGFDNIPQTAEEFNFAPNKTFKLIVYSLLAASLTYVVMILYTGWLSTSHQSLNGQLWLTGAVTQTAFGYIGLGVLAIAIMMGIFTGLNGFLMSSSRLLFSMGRSGIMPTMFSKLHSKYKTPYVAIIFLVGVSLIAPWLGRTALTWIVDMSSTGVSIAYFITCLSAAKLFSYNKQSNTYAPVYKTFAIIGSFVSFIFLALLLVPGSPAALTAPSYIALLGWLIIGLIFFVIRYPKLKNMDNDELSRLILNRSENEVDDMIEEPEKEKTK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• data available for COL, NCTC8325, Newman

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications