PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA0698 [new locus tag: SA_RS03975 ]
• pan locus tag^?: SAUPAN002650000
• symbol: pepT
• pan gene symbol^?: pepT
• synonym:
• product: peptidase T

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA0698 [new locus tag: SA_RS03975 ]
• symbol: pepT
• product: peptidase T
• replicon: chromosome
• strand: -
• coordinates: 796283..797509
• length: 1227
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1123505 NCBI
• RefSeq: NP_373953 NCBI
• BioCyc: see SA_RS03975
• MicrobesOnline: 102979 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  1021
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  1201
  
  ATGAAGAACCAATTGATAGATAGATTAACAAGATATACGACAATTGATACGCAATCTGAT
  CCAAAATCTACAACAACACCTTCAACTGAAAAGCAATGGGATTTGTTACATTTATTAGAA
  AAAGAATTACAACAATTAGGATTGCCAACTGATTTAGATGAAAATGGCTACTTATTTGCT
  ACATTAGAAAGTAACATCGATGCCGACGTCCCAACAGTTGGATTTTTGGCGCATGTTGAT
  ACATCACCTGATTTCAATGCTTCAAATGTTAAACCGCAAATTATTGAAAACTATGATGGT
  AAGCCATACAAACTAGGCAATACTAAACGTGTCTTAGATCCTAAAGTATTTCCAGAACTT
  AACAGTTTAGTTGGTCATACATTAATGGTAACTGATGGTACATCGTTACTAGGTGCAGAC
  GATAAAGCGGGTATTGTGGAAATTATGGAAGCTATTTGTTATTTACAAGAACATCCAGAA
  ATTAAACATGGTACCATTCGCATTGGATTTACACCAGACGAAGAAATCGGTCGTGGTCCA
  CATAAATTTGATGTTGACCGCTTCAATGCTGATTTTGCTTATACTATGGATGGTAGTCAA
  TATGGAGAATTACAATATGAAAGCTTTAACGCTGCTGAAGCGGTTATTACATGCCACGGT
  GTAAATGTTCATCCTGGTTCAGCTAAAAATGCAATGGTAAACGCAATACGTTTAGGTGAA
  CAATTCGATAGTTTGCTACCTGATAGTGAAGTTCCGGAGCGAACAGAAGGATACGAAGGC
  TTTTATCACTTAATGAACTTTGAAGGAACTGTTGAAAAAGCAACTTTGCAATACATTATT
  CGTGATCATGATAAAAAACAATTCGAATTGCGTAAGAAACGTATTTTAGAAATACGTGAC
  GATATCAATGCCCATTTTGAAAATTATCCAGTTAAAGTTGATATATCGGATCAATATTTC
  AATATGGCAGAAAAAATATTACCATTGCCTCATATTATTGATATACCTAAACGTGTCTTT
  GCCAAATTAGATATTCCAGCAAACACTGAACCTATTCGTGGTGGTACAGATGGTTCACAA
  TTGTCATTTATGGGGTTACCAACTCCAAACATCTTTACAGGTTGCGGTAACTTCCACGGC
  CCATATGAATATGCATCCATTGATGTTATGGAAAAAGCAGTACAAGTAATCATTGGAATT
  GTAGAAGATATCGCTGAAAATCACTAA
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  1227
  

⊟Protein

⊟General

• locus tag: SA0698 [new locus tag: SA_RS03975 ]
• symbol: PepT
• description: peptidase T
• length: 408
• theoretical pI: 4.56391
• theoretical MW: 45819.3
• GRAVY: -0.404167

⊟Function

• reaction:
  EC 3.4.11.4?  ExPASy
  Tripeptide aminopeptidase Release of the N-terminal residue from a tripeptide
• TIGRFAM:
  Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides peptidase T (TIGR01882; EC 3.4.11.-; HMM-score: 554.4)
  and 7 more
  peptidase T-like protein (TIGR01883; HMM-score: 106.3)
  Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides peptidase, ArgE/DapE family (TIGR01910; EC 3.4.-.-; HMM-score: 24.3)
  Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides amidohydrolase (TIGR01891; HMM-score: 14.7)
  Xaa-His dipeptidase (TIGR01893; EC 3.4.13.20; HMM-score: 13.2)
  amidase, hydantoinase/carbamoylase family (TIGR01879; EC 3.5.-.-; HMM-score: 11.2)
  hydrolase, peptidase M42 family (TIGR03106; HMM-score: 11.1)
  N-acyl-L-amino-acid amidohydrolase (TIGR01880; EC 3.5.1.14; HMM-score: 10.5)
• TheSEED  :
  • Tripeptide aminopeptidase (EC 3.4.11.4)
  Stress Response Oxidative stress Glutathione: Biosynthesis and gamma-glutamyl cycle  Tripeptide aminopeptidase (EC 3.4.11.4)
• PFAM:
  Peptidase_MH (CL0035) Peptidase_M20; Peptidase family M20/M25/M40 (PF01546; HMM-score: 46.9)
  no clan defined M20_dimer; Peptidase dimerisation domain (PF07687; HMM-score: 37.9)
  and 1 more
  Peptidase_MH (CL0035) Peptidase_M42; M42 glutamyl aminopeptidase (PF05343; HMM-score: 25.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors: Zn²⁺
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.005271
  • TAT(Tat/SPI): 0.00067
  • LIPO(Sec/SPII): 0.000664
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15926420 NCBI
• RefSeq: NP_373953 NCBI
• UniProt: P65806 UniProt

⊟Protein sequence

• MKNQLIDRLTRYTTIDTQSDPKSTTTPSTEKQWDLLHLLEKELQQLGLPTDLDENGYLFATLESNIDADVPTVGFLAHVDTSPDFNASNVKPQIIENYDGKPYKLGNTKRVLDPKVFPELNSLVGHTLMVTDGTSLLGADDKAGIVEIMEAICYLQEHPEIKHGTIRIGFTPDEEIGRGPHKFDVDRFNADFAYTMDGSQYGELQYESFNAAEAVITCHGVNVHPGSAKNAMVNAIRLGEQFDSLLPDSEVPERTEGYEGFYHLMNFEGTVEKATLQYIIRDHDKKQFELRKKRILEIRDDINAHFENYPVKVDISDQYFNMAEKILPLPHIIDIPKRVFAKLDIPANTEPIRGGTDGSQLSFMGLPTPNIFTGCGNFHGPYEYASIDVMEKAVQVIIGIVEDIAENH

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: pepT < SA0699 < SA0700

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications