PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA0846 [new locus tag: SA_RS04800 ]
• pan locus tag^?: SAUPAN003134000
• symbol: SA0846
• pan gene symbol^?: opp3C
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA0846 [new locus tag: SA_RS04800 ]
• symbol: SA0846
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 958942..960012
• length: 1071
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1123663 NCBI
• RefSeq: NP_374109 NCBI
• BioCyc: see SA_RS04800
• MicrobesOnline: 103135 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  1021
  
  ATGGCTGAAAATAAAAACAATTTGTCGATTAACGACGATCATTCTAATGCAGCTATGACG
  CATACCTCTGACGCTATCGCATCATCTGATTTTATTATTAGAGAATTAGACTTGAATCAG
  GAACCTGAAATGCAACGAGAAAGCAAAAACTTTTGGCAAGATGCTTGGGCTCAGTTAAAA
  CGAAATAAGTTAGCTGTTGTCGGTATGATAGGTTTAATTATCATTGTAATATTTGCTTTT
  ATCGGTCCAGTTATAAATAAACATGATTATGCTGAACAAAATGTAGAACATAGAAATCTT
  CCGGCAAAAATACCTGTATTAGACAAAGTTCCATTTTTACCTTTTGATGGTAAAGATGCA
  GATGGCAAGGATGCTTATAAAGCAGCAAATGCTAAAGAAAATTATTGGTTTGGTACTGAT
  CAGTTGGGTCGAGATTTATGGACAAGAACATGGAAAGGTGCTCAAATTTCATTGTTTATC
  GGTGTTGTTGCAGCGATGTTAGATATTTTTATTGGCGTTGTATATGGTGCGATTTCTGGA
  TTCTTCGGTGGACGTGTCGATACGATTATGCAACGTATACTTGAAGTCATAGCATCTATT
  CCGAATTTAATTGTCGTAATTTTATTTGTATTAATTTTTGAACCATCCATTTGGACAATT
  ATATTGGCTATGTCTATCACAGGCTGGTTAGGCATGAGCAGAGTTGTACGTGGAGAATTT
  TTAAAATTGAAAAATCAAGAGTTTGTCATGGCTTCGAAAACATTGGGGGCTTCAAAATTC
  AAATTGATATTTAAGCATATTTTACCTAATACGTTAGGTGCTATCGTGGTTACATCAATG
  TTTACAGTACCTAGTGCTATTTTCTTCGAAGCATTTTTAAGTTTCATTGGTATAGGTGTA
  CCTGCACCTCAAACATCGTTAGGGTCATTAGTAAATGATGGGCGCGCAATGTTATTAATT
  TATCCACATGAATTATTTATACCAGCAATGATTTTAAGTTTATTAATTCTATTCTTTTAC
  TTATTTAGTGATGGATTACGTGATGCATTTGATCCGAAAATGCGTAAATAA
  60
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  1071
  

⊟Protein

⊟General

• locus tag: SA0846 [new locus tag: SA_RS04800 ]
• symbol: SA0846
• description: hypothetical protein
• length: 356
• theoretical pI: 7.18495
• theoretical MW: 39765.4
• GRAVY: 0.35309

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Cations and iron carrying compounds nickel ABC transporter, permease subunit NikC (TIGR02790; EC 3.6.3.24; HMM-score: 196.3)
  and 3 more
  Metabolism Transport and binding proteins Anions phosphonate ABC transporter, permease protein PhnE (TIGR01097; HMM-score: 30.8)
  Metabolism Transport and binding proteins Anions phosphate ABC transporter, permease protein PstA (TIGR00974; HMM-score: 17.4)
  Metabolism Transport and binding proteins Anions phosphate ABC transporter, permease protein PstC (TIGR02138; HMM-score: 13.5)
• TheSEED  :
  • Oligopeptide ABC transporter (EC 7.4.2.6), permease protein OppC
  Membrane Transport ABC transporters ABC transporter oligopeptide (TC 3.A.1.5.1)  Oligopeptide transport system permease protein OppC (TC 3.A.1.5.1)
• PFAM:
  BPD_transp_1 (CL0404) BPD_transp_1; Binding-protein-dependent transport system inner membrane component (PF00528; HMM-score: 104.2)
  and 3 more
  no clan defined OppC_N; N-terminal TM domain of oligopeptide transport permease C (PF12911; HMM-score: 60.3)
  SNARE-fusion (CL0445) Synaptobrevin; Synaptobrevin (PF00957; HMM-score: 7)
  no clan defined Pox_A14; Poxvirus virion envelope protein A14 (PF05767; HMM-score: 6.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 6
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.003312
  • TAT(Tat/SPI): 0.000473
  • LIPO(Sec/SPII): 0.000519
• predicted transmembrane helices (TMHMM): 6

⊟Accession numbers

• GI: 15926576 NCBI
• RefSeq: NP_374109 NCBI
• UniProt: A0A0H3JM52 UniProt

⊟Protein sequence

• MAENKNNLSINDDHSNAAMTHTSDAIASSDFIIRELDLNQEPEMQRESKNFWQDAWAQLKRNKLAVVGMIGLIIIVIFAFIGPVINKHDYAEQNVEHRNLPAKIPVLDKVPFLPFDGKDADGKDAYKAANAKENYWFGTDQLGRDLWTRTWKGAQISLFIGVVAAMLDIFIGVVYGAISGFFGGRVDTIMQRILEVIASIPNLIVVILFVLIFEPSIWTIILAMSITGWLGMSRVVRGEFLKLKNQEFVMASKTLGASKFKLIFKHILPNTLGAIVVTSMFTVPSAIFFEAFLSFIGIGVPAPQTSLGSLVNDGRAMLLIYPHELFIPAMILSLLILFFYLFSDGLRDAFDPKMRK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: oppB > SA0846 > oppD > oppF > SA0849

⊟Regulation

• regulator: CodY (repression) regulon

  CodY

  (TF)

  important in Amino acid metabolism; RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Aurelia Hiron, Elise Borezée-Durant, Jean-Christophe Piard, Vincent Juillard
Only one of four oligopeptide transport systems mediates nitrogen nutrition in Staphylococcus aureus.
J Bacteriol: 2007, 189(14);5119-29
[PubMed:17496096] [WorldCat.org] [DOI] (P p)