From AureoWiki
Jump to navigation Jump to search
PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0850 [new locus tag: SA_RS04820 ]
  • pan locus tag?: SAUPAN003146000
  • symbol: SA0850
  • pan gene symbol?: opp4A
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA0850 [new locus tag: SA_RS04820 ]
  • symbol: SA0850
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 963927..965642
  • length: 1716
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGGGAAGCTAATTAAATATATTTCAATACTTCTTATTGTCGTTTTAGTGTTGAGTGCT
    TGCGGAAAAAGCAGTAATAAAGATGAAGGAGTAAAAGATGCTACTAAAACGGAAACCTCA
    AAACATAAAGGTGGTACCTTAAATGTAGCATTAACAGCACCGCCAAGTGGTGTTTATTCT
    TCGTTATTAAATAGTACACATGCAGATTCTGTAGTTGAGGGATATTTTAACGAAAGCTTA
    TTAGCAACTGATAAAAAAATACGTCCTAAGGCATATATTGCTTCATGGAAGGACATCGAG
    CCGGCTAAGAAAATAGAATTTAAAATTAAAAAAGGTATTAAATGGCATGATGGTAATGAA
    TTGAAAATTGATGATTGGATTTATTCAATTGAAGTCTTAGCTAACAAGGACTACGAAGGT
    GCTTATTATCCAAGTGTAGAAAATATCCAAGGTGCGAAAGATTATCATGAAGGAAAAACT
    GATCATATTAGCGGATTGAAGAAAATAGATGACTACACTATGCAGGTTACATTTGATAAA
    AAACAAGAAAATTACTTAACAGGATTTATTACTGGACATTTATTAAGTAAAAAATATTTA
    TCAGATGTACCAATTAAAGATTTAGCGAAATCAGATAAAATCCGAAAATATCCTATTGGT
    ATTGGACCGTATAAAGTTAAGAAAATCGTTCCAGGTGAGGCTGTTCAACTCGTTAAATTT
    GATGATTATTGGCAAGGTAAGCCTGCACTAGACAAAATCAATTTAAAAGTTATTGATCAA
    GCTCAAATTATTAAGGCAATGGAAAAAGGCGATATTGATGTTGCGAATGATGCTACCGGT
    GCAATGGCAAAAGATGCTAAGTCATCTAATGCTGGTCTCAAGGTATTATCTGCGCCAAGC
    TTAGACTACGGTTTAATAGGATTCGTATCTCATGATTACGATAAAAAAGCTAATAAAACT
    GGTAAAGTGAGACCAAAATATGAAGACAAAGAATTACGTAAAGCAATGCTTTATGCAATT
    GATAGAGAAAAATGGATTAAAGCGTTTTTCAATGGTTACGCTAGTGAAATCAATAGTTTT
    GTACCATCTATGCATTGGATAGCAGCCAATCCTAAGGACCTAAATGATTACAAATATGAT
    CCTGAAAAAGCTAAAAAAATCTTAGATAAGTTAGGTTATAAAGATAGAGATGGTGACGGA
    TTTAGAGAAGATCCTAAAGGTAATAAATTTGAGATTAACTTTAAACATTATTCAGGTTCA
    AATCCTACTTTTGAACCAAGAACTGCTGCGATAAAAGATTTCTGGGAAAAAGTTGGCTTG
    AAAACAAATGTGAAGTTAGTAGAATTCGGTAAATATAATGAAGACTTAGCAAATGCATCT
    AAAGATATGGAAGTGTACTTCAGATCATGGGCAGGAGGTACAGATCCAGATCCATCAGAT
    TTATACCACACTGATAGACCTCAAAATGAAATGAGAACAGTTTTACCAAAATCAGATCAA
    TATTTAGATGATGCATTAGACTTCGAAAAAGTAGGCATTGATGAAAAGAAACGTAAAGAT
    ATTTATGTTAAATGGCAAAAATATATGAATGATGAGTTACCTGGATTACCAATGTTCCAA
    GGTAAATCGATAACTATTGTTAACGATAAAGTACGAAACTTAGACATTGAAATTGGAACT
    GATCAAAGTTTATATAATTTAACTAAAGAAGCTTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1716

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA0850 [new locus tag: SA_RS04820 ]
  • symbol: SA0850
  • description: hypothetical protein
  • length: 571
  • theoretical pI: 9.00454
  • theoretical MW: 64616.1
  • GRAVY: -0.639405

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Cations and iron carrying compounds nickel ABC transporter, nickel/metallophore periplasmic binding protein (TIGR02294; HMM-score: 94.2)
    Metabolism Transport and binding proteins Unknown substrate ABC transporter substrate binding protein, KPN_01854 family (TIGR04028; HMM-score: 79.2)
  • TheSEED  :
    • Oligopeptide ABC transporter (EC 7.4.2.6), substrate-binding protein OppA
    Membrane Transport ABC transporters ABC transporter oligopeptide (TC 3.A.1.5.1)  Oligopeptide ABC transporter, periplasmic oligopeptide-binding protein OppA (TC 3.A.1.5.1)
    and 1 more
    Regulation and Cell signaling Regulation and Cell signaling - no subcategory Sex pheromones in Enterococcus faecalis and other Firmicutes  Oligopeptide ABC transporter, periplasmic oligopeptide-binding protein OppA (TC 3.A.1.5.1)
  • PFAM:
    PBP (CL0177) SBP_bac_5; Bacterial extracellular solute-binding proteins, family 5 Middle (PF00496; HMM-score: 265.2)
    and 1 more
    GBD (CL0202) P_proprotein; Proprotein convertase P-domain (PF01483; HMM-score: 12.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 3.33
    • Cellwall Score: 3.33
    • Extracellular Score: 3.33
    • Internal Helices: 0
  • LocateP: Lipid anchored
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPII)
    • Intracellular possibility: 0
    • Signal peptide possibility: 0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: LVLSACG
  • SignalP: Signal peptide LIPO(Sec/SPII) length 20 aa
    • SP(Sec/SPI): 0.000481
    • TAT(Tat/SPI): 0.000083
    • LIPO(Sec/SPII): 0.99921
    • Cleavage Site: CS pos: 20-21. LSA-CG. Pr: 0.9997
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MGKLIKYISILLIVVLVLSACGKSSNKDEGVKDATKTETSKHKGGTLNVALTAPPSGVYSSLLNSTHADSVVEGYFNESLLATDKKIRPKAYIASWKDIEPAKKIEFKIKKGIKWHDGNELKIDDWIYSIEVLANKDYEGAYYPSVENIQGAKDYHEGKTDHISGLKKIDDYTMQVTFDKKQENYLTGFITGHLLSKKYLSDVPIKDLAKSDKIRKYPIGIGPYKVKKIVPGEAVQLVKFDDYWQGKPALDKINLKVIDQAQIIKAMEKGDIDVANDATGAMAKDAKSSNAGLKVLSAPSLDYGLIGFVSHDYDKKANKTGKVRPKYEDKELRKAMLYAIDREKWIKAFFNGYASEINSFVPSMHWIAANPKDLNDYKYDPEKAKKILDKLGYKDRDGDGFREDPKGNKFEINFKHYSGSNPTFEPRTAAIKDFWEKVGLKTNVKLVEFGKYNEDLANASKDMEVYFRSWAGGTDPDPSDLYHTDRPQNEMRTVLPKSDQYLDDALDFEKVGIDEKKRKDIYVKWQKYMNDELPGLPMFQGKSITIVNDKVRNLDIEIGTDQSLYNLTKEA

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CodY (repression) regulon
    CodY(TF)important in Amino acid metabolism; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SA0850&oldid=86834"