From AureoWiki
Jump to navigation Jump to search

NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1228 [new locus tag: SA_RS06975 ]
  • pan locus tag?: SAUPAN003809000
  • symbol: dapB
  • pan gene symbol?: dapB
  • synonym:
  • product: dihydrodipicolinate reductase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA1228 [new locus tag: SA_RS06975 ]
  • symbol: dapB
  • product: dihydrodipicolinate reductase
  • replicon: chromosome
  • strand: +
  • coordinates: 1402887..1403609
  • length: 723
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    GTGAAAATATTACTAATTGGCTATGGTGCAATGAATCAACGCGTTGCTAGATTAGCAGAA
    GAAAAAGGACATGAAATCGTTGGGGTCATTGAAAATACACCGAAAGCAACAACGCCATAT
    CAACAATATCAACATATTGCAGATGTTAAAGATGCCGATGTTGCAATAGATTTTTCAAAT
    CCGAATCTATTATTCCCATTATTAGATGAAGAGTTTCATTTGCCATTAGTTGTGGCAACA
    ACTGGCGAGAAAGAAAAACTACTTAATAAGTTAGATGAATTGAGTCAAAATATGCCTGTA
    TTTTTCAGCGCGAACATGAGTTATGGCGTTCATGCACTGACTAAAATTTTAGCAGCTGCT
    GTTCCCCTACTTGATGATTTCGACATCGAATTGACTGAGGCACATCATAATAAAAAAGTA
    GATGCACCAAGTGGTACGTTAGAAAAATTGTATGATGTGATCGTATCTTTGAAAGAAAAT
    GTAACACCTGTGTATGATAGACATGAATTAAATGAAAAACGCCAGCCACAAGATATTGGT
    ATACATTCTATTCGTGGAGGTACGATTGTCGGTGAACATGAAGTTCTATTTGCTGGCACT
    GATGAAACGATTCAAATCACACATCGTGCACAATCAAAAGATATTTTTGCGAATGGTGCT
    ATACAAGCAGCAGAACGCTTAGTTAATAAACCAAACGGCTTTTATACGTTTGACAACTTA
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    723

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA1228 [new locus tag: SA_RS06975 ]
  • symbol: DapB
  • description: dihydrodipicolinate reductase
  • length: 240
  • theoretical pI: 5.00438
  • theoretical MW: 26726.1
  • GRAVY: -0.235417

⊟Function[edit | edit source]

  • reaction:
    EC 1.17.1.8?  ExPASy
    4-hydroxy-tetrahydrodipicolinate reductase (S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + NAD(P)+ + H2O = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NAD(P)H
  • TIGRFAM:
    Metabolism Amino acid biosynthesis Aspartate family 4-hydroxy-tetrahydrodipicolinate reductase (TIGR00036; EC 1.17.1.8; HMM-score: 168.1)
    and 2 more
    Metabolism Amino acid biosynthesis Aspartate family dihydrodipicolinate reductase (TIGR02130; EC 1.17.1.8; HMM-score: 24.3)
    Metabolism Energy metabolism Pentose phosphate pathway 6-phosphogluconate dehydrogenase (decarboxylating) (TIGR00872; EC 1.1.1.44; HMM-score: 15)
  • TheSEED  :
    • 4-hydroxy-tetrahydrodipicolinate reductase (EC 1.17.1.8)
    Amino Acids and Derivatives Lysine, threonine, methionine, and cysteine Lysine Biosynthesis DAP Pathway  4-hydroxy-tetrahydrodipicolinate reductase (EC 1.17.1.8)
  • PFAM:
    NADP_Rossmann (CL0063) DapB_C; Dihydrodipicolinate reductase, C-terminus (PF05173; HMM-score: 130.2)
    and 3 more
    DapB_N; Dihydrodipicolinate reductase, N-terminus (PF01113; HMM-score: 58)
    F420_oxidored; NADP oxidoreductase coenzyme F420-dependent (PF03807; HMM-score: 13.7)
    TPR (CL0020) Cse1; Cse1 (PF08506; HMM-score: 12.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.018186
    • TAT(Tat/SPI): 0.000324
    • LIPO(Sec/SPII): 0.002585
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKILLIGYGAMNQRVARLAEEKGHEIVGVIENTPKATTPYQQYQHIADVKDADVAIDFSNPNLLFPLLDEEFHLPLVVATTGEKEKLLNKLDELSQNMPVFFSANMSYGVHALTKILAAAVPLLDDFDIELTEAHHNKKVDAPSGTLEKLYDVIVSLKENVTPVYDRHELNEKRQPQDIGIHSIRGGTIVGEHEVLFAGTDETIQITHRAQSKDIFANGAIQAAERLVNKPNGFYTFDNL

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: L-box (transcription termination) regulon, CodY (repression) regulon
    L-box(RNA)important in Lysine biosynthesis; RegPrecise 
    CodY(TF)important in Amino acid metabolism; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SA1228&oldid=94714"