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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1255 [new locus tag: SA_RS07120 ]
  • pan locus tag?: SAUPAN003853000
  • symbol: SA1255
  • pan gene symbol?: crr
  • synonym:
  • product: PTS system glucose-specific transporter subunit enzyme II A

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA1255 [new locus tag: SA_RS07120 ]
  • symbol: SA1255
  • product: PTS system glucose-specific transporter subunit enzyme II A
  • replicon: chromosome
  • strand: -
  • coordinates: 1429500..1430000
  • length: 501
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    ATGTTTAAAAAATTATTCGGAAAAGGCAAAGAAGTTCAGAAAGATATCGCAATTTATGCA
    CCATTAACTGGAGAATACGTGAAAATTGAAGATATTCCAGATCCTGTATTCGCACAAAAA
    ATGATGGGCGAAGGTTTTGGTATTAATCCAACTGAAGGAGAAGTTGTGTCTCCAATTGCA
    GGACGTGTTGACAATGTCTTTCCAACTAAGCATGCGATTGGGTTAAAAGCAGATAACGGA
    TTAGAATTATTAGTTCATATCGGTTTAGACACAGTTCAATTAGACGGTGAAGGATTTGAA
    GTGTTAGTGTCTAGTGGTGACGAAGTTAACGTCGGAGATCCATTAGTAAGATTCAACCTT
    GAATTTATTAATAATAACGCTAAATCTGTTATTTCACCAATTATAATTACTAACACTGAT
    CAAGCAGCTTCAATTAATATTTATGATGAAAATGCTGTGATTAAAGGTGAAACAAAAGTG
    ATTGATGTGACAATGAACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    501

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA1255 [new locus tag: SA_RS07120 ]
  • symbol: SA1255
  • description: PTS system glucose-specific transporter subunit enzyme II A
  • length: 166
  • theoretical pI: 4.26969
  • theoretical MW: 17960.4
  • GRAVY: 0.0162651

⊟Function[edit | edit source]

  • TIGRFAM:
    PTS system, beta-glucoside-specific IIABC component (TIGR01995; EC 2.7.1.69; HMM-score: 181.2)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, glucose subfamily, IIA component (TIGR00830; HMM-score: 151.8)
    Signal transduction Signal transduction PTS PTS system, glucose subfamily, IIA component (TIGR00830; HMM-score: 151.8)
    and 1 more
    Genetic information processing Protein fate Protein and peptide secretion and trafficking type I secretion membrane fusion protein, HlyD family (TIGR01843; HMM-score: 11.6)
  • TheSEED  :
    • PTS system, IIA component YpqE
    Carbohydrates Di- and oligosaccharides Trehalose Uptake and Utilization  PTS system, glucose-specific IIA component
  • PFAM:
    Hybrid (CL0105) PTS_EIIA_1; phosphoenolpyruvate-dependent sugar phosphotransferase system, EIIA 1 (PF00358; HMM-score: 177.2)
    and 2 more
    RnfC_N; RnfC Barrel sandwich hybrid domain (PF13375; HMM-score: 18.7)
    Biotin_lipoyl; Biotin-requiring enzyme (PF00364; HMM-score: 13.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Zn2+
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.011963
    • TAT(Tat/SPI): 0.000525
    • LIPO(Sec/SPII): 0.001159
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MFKKLFGKGKEVQKDIAIYAPLTGEYVKIEDIPDPVFAQKMMGEGFGINPTEGEVVSPIAGRVDNVFPTKHAIGLKADNGLELLVHIGLDTVQLDGEGFEVLVSSGDEVNVGDPLVRFNLEFINNNAKSVISPIIITNTDQAASINIYDENAVIKGETKVIDVTMN

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Alexander Scherl, Patrice François, Manuela Bento, Jacques M Deshusses, Yvan Charbonnier, Véronique Converset, Antoine Huyghe, Nadia Walter, Christine Hoogland, Ron D Appel, Jean-Charles Sanchez, Catherine G Zimmermann-Ivol, Garry L Corthals, Denis F Hochstrasser, Jacques Schrenzel
Correlation of proteomic and transcriptomic profiles of Staphylococcus aureus during the post-exponential phase of growth.
J Microbiol Methods: 2005, 60(2);247-57
[PubMed:15590099] [WorldCat.org] [DOI] (P p)

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